Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Latar belakang: Studi ini dilakukan untuk mempelajari pengaruh pemberian ekstrak akar Acalypha indica Linn terhadap viabilitas relatif dan proliferasi sel sebagai parameter neurogenesis pada kultur jaringan hipokampus tikus pascahipoksia. Metode: Studi eksperimental in vitro pada 24 kultur primer jaringan sel saraf tikus Sprague Dowley dewasa yang dipajankan terhadap hipoksia dengan gas 5% O2/5% CO2/N2 seimbang selama 24 jam. Pascahipoksia, ekstrak Acalypha indica Linn ditambahkan pada 3 kelompok perlakuan, masing-masing dengan dosis 10, 15, dan 20 mg/mL, sedangkan pada kelompok kontrol tidak ditambahkan apapun. Setiap kelompok terdiri atas 6 sampel. Setelah inkubasi selama 90 jam, viabilitas relatif sel diukur dengan 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), proliferasi sel diukur dengan 5-bromo2â??-deoxy-uridine (BrdU). Data dianalisis dengan menggunakan tes parametrik one way ANOVA yang dilanjutkan dengan analisis post-hoc. Hasil: Viabilitas relatif sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (176,95%, 220,62%, 386,02% vs. 100%). Proliferasi sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (0,132; 0,117; 0,114 vs. 0,096). Kesimpulan: Ekstrak Acalypha indica Linn dapat meningkatkan viabilitas relatif dan proliferasi sel pascahipoksia in vitro pada dosis 10, 15, dan 20 mg/mL. (Med J Indones 2011; 20:94-9)

---

Abstract
Background: This research was done to study the infl uence of Acalypha indica Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture. Methods Experimental in vitro study using 24 primary neuronal cell cultures obtained from adult Sprague Dawley rat exposed to hypoxia with 5% O2/5% CO2/N2 balance gas for 24 hours. Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. No treatment was given to the control group. Each group consists of 6 samples. After 90 hours of incubation, relative cell viability was measured by using 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and cell proliferation was measured by using 5-bromo2â??-deoxy-uridine (BrdU) for cell proliferation. Data was analyzed using one way ANOVA parametric tests, then further analyzed with post-hoc analysis. Results: The relative cell viability of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). Cell proliferation of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (0.132, 0.117, 0.114 vs 0.096). Conclusion: Acalypha indica Linn root extract with doses of 10, 15, and 20 mg/mL can increase relative cell viability and proliferation in post-hypoxic hippocampal tissue culture. (Med J Indones 2011; 20:94-9)

Abstrak :
The studies of neuro-protection and neuro-therapy effects of Acalypha indica Linn. water extract ex vivo on Musculus gastrocnemius frog have already done at three Departments in Faculty of Medicine, University of Indonesia. The experimental studies were done on 2 groups of frog for neuro-protection and neuro-therapy effects. Each group of frog was divided into 7 subgroups of application, 4 samples each. There were 5 subgroups of doses: 5; 10; 15; 20; 25 mg and 2 subgroups as control. Pancuronium bromide 0.2%, 4 mg, was used for a positive control as muscle relaxant. Neuroprotection study was done as follow: ringer - extract - pancuronium bromide, and neuro-therapy study was ringer - pancuronium bromide - extract, respectively. The parameters measured in these studies were the electrical activities such as amount and duration (second) of re-polarization; depolarization, resting potential, and the height of spike after electrical stimulation at 5 mV. Neuro-protection effect of extract was determined by the ability of muscle to show the electrical response after incubating with pancuronium bromide for 10 minutes, and after incubating with extract for 10 minutes for neuro-therapy effect. In the dose of 15 mg and 20 mg/mL of A. indica Linn. extract showed better activities than the dose of 25 mg of extract, both as neuro-protection and neuro-therapy effects, but statistically its have not a significant difference. This study should be followed by an in vivo experiment on frog and it would be done in pharmacokinetic and pharmacodynamic studies on other animal models.

Abstrak :
Kadar matrix metalloproteinase?9 (MMP-9) yang tinggi diyakini merusak sawar darah otak (SDO) sehingga terjadi edema serebri dan menambah lama rawat inap pasien. Penelitian pada binatang menunjukkan tigesiklin menurunkan kadar MMP-9. Kemampuan tigesiklin menurunkan kadar MMP-9 yang berdampak pada pengurangan edema serebri dan lama rawat inap pasien perdarahan.