Hasil Pencarian  ::  Simpan CSV :: Kembali

Abstrak :
Deteksi Infeksi Submikroskopis Necator americanus, Ancylostoma duodenale, dan Ascaris lumbricoides dari Sampel Feses di Nangapanda, Ende, Menggunakan Real-Time Polymerase Chain Reaction. Infeksi dari Soil-Transmitted Helminthes (STH) (N. americanus, A. duodenale (Hookworm), dan A. lumbricoides) dapat menyebabkan anemia, kekurangan zat besi, bahkan malnutrisi. Pemeriksaan infeksi STH dapat dilakukan menggunakan mikroskop, tetapi metode tersebut masih kurang sensitif. Penelitian bertujuan mendeteksi dan mengetahui persentase infeksi submikroskopis STH dari sampel feses anak (usia 5-18 tahun) di Nangapanda, Ende menggunakan metode real-time polymerase chain reaction (PCR). Sampel feses dikoleksi sebanyak dua kali, yaitu sebelum dan sesudah pemberian albendazole 400 mg. Total sampel yang diperoleh adalah 242 tetapi hanya 45 sampel yang negatif secara mikroskopis yang diuji dengan real-time PCR. DNA sampel diisolasi dan diamplifikasi menggunakan primer dari daerah internal transcribed spacer (ITS-1 dan ITS-2) rDNA. Deteksi dengan real-time PCR menghasilkan kurva amplifikasi pada fluorophore VIC, FAM, dan Texas Red. Sebanyak tiga sampel (6,7%) pada pre treatment termasuk low load of DNA (N. americanus and A. lumbricoides) (Ct > 35), empat sampel (9,1%) termasuk low load of DNA untuk N. americanus saja (Ct > 35), dan lima sampel (11,4%) termasuk moderate load of DNA untuk A. lumbricoides saja (30 < Ct < 35) pada post treatment. Hasil penelitian menunjukkan bahwa real-time PCR dapat mendeteksi infeksi submikroskopis dari Hookworm dan A. lumbricoides.

---

Soil-transmitted helminth (STH) infections (Necator americanus (hookworm), Ancylostoma duodenale (hookworm), and Ascaris lumbricoides) can lead to anemia, malnutrition, and iron deficiency. Traditionally, STH infections have been diagnosed using microscopy to detect eggs in human fecal samples. However, there are several limitations of this method. The aim of this research was to detect the percentage of submicroscopic STH infections from human fecal samples (children, 5?18 years old) in Nangapanda, Ende, using the real-time polymerase chain reaction (PCR) method. The fecal samples were collected in two time periods, which were before and after treatment, using 400 mg of Albendazole. There were 242 samples in total, but only 45 negative samples from microscopic detection were tested with real-time PCR. The DNA samples were isolated and amplified wih primers of internal transcribed spacer (ITS-1 and ITS-2) region of rDNA. The detection of samples with real-time PCR generated an amplification curve in VIC, FAM, and Texas Red fluorophore. Three samples (6.7%) in pre-treatment were low load of DNA (N. americanus and A. lumbricoides) (Ct > 35). Four samples (9.1%) were low load of DNA (N. americanus) (Ct > 35) in post-treatment. Five samples (11.4%) were moderate load of DNA (A. lumbricoides) (30 < Ct < 35) in post-treatment. real-time PCR could detect submicroscopic infections from specific species of hookworm and A. lumbricoides.

Abstrak :
Deteksi Infeksi Submikroskopis Necator americanus, Ancylostoma duodenale, dan Ascaris lumbricoides dari Sampel Feses di Nangapanda, Ende, Menggunakan Real-Time Polymerase Chain Reaction. Infeksi dari Soil-Transmitted Helminthes (STH) (N. americanus, A. duodenale (Hookworm), dan A. lumbricoides) dapat menyebabkan anemia, kekurangan zat besi, bahkan malnutrisi. Pemeriksaan infeksi STH dapat dilakukan menggunakan mikroskop, tetapi metode tersebut masih kurang sensitif. Penelitian bertujuan mendeteksi dan mengetahui persentase infeksi submikroskopis STH dari sampel feses anak (usia 5-18 tahun) di Nangapanda, Ende menggunakan metode real-time polymerase chain reaction (PCR). Sampel feses dikoleksi sebanyak dua kali, yaitu sebelum dan sesudah pemberian albendazole 400 mg. Total sampel yang diperoleh adalah 242 tetapi hanya 45 sampel yang negatif secara mikroskopis yang diuji dengan real-time PCR. DNA sampel diisolasi dan diamplifikasi menggunakan primer dari daerah internal transcribed spacer (ITS-1 dan ITS-2) rDNA. Deteksi dengan real-time PCR menghasilkan kurva amplifikasi pada fluorophore VIC, FAM, dan Texas Red. Sebanyak tiga sampel (6,7%) pada pre treatment termasuk low load of DNA (N. americanus and A. lumbricoides) (Ct > 35), empat sampel (9,1%) termasuk low load of DNA untuk N. americanus saja (Ct > 35), dan lima sampel (11,4%) termasuk moderate load of DNA untuk A. lumbricoides saja (30 < Ct < 35) pada post treatment. Hasil penelitian menunjukkan bahwa real-time PCR dapat mendeteksi infeksi submikroskopis dari Hookworm dan A. lumbricoides.

---

Soil-transmitted helminth (STH) infections (Necator americanus (hookworm), Ancylostoma duodenale (hookworm), and Ascaris lumbricoides) can lead to anemia, malnutrition, and iron deficiency. Traditionally, STH infections have been diagnosed using microscopy to detect eggs in human fecal samples. However, there are several limitations of this method. The aim of this research was to detect the percentage of submicroscopic STH infections from human fecal samples (children, 5?18 years old) in Nangapanda, Ende, using the real-time polymerase chain reaction (PCR) method. The fecal samples were collected in two time periods, which were before and after treatment, using 400 mg of Albendazole. There were 242 samples in total, but only 45 negative samples from microscopic detection were tested with real-time PCR. The DNA samples were isolated and amplified wih primers of internal transcribed spacer (ITS-1 and ITS-2) region of rDNA. The detection of samples with real-time PCR generated an amplification curve in VIC, FAM, and Texas Red fluorophore. Three samples (6.7%) in pre-treatment were low load of DNA (N. americanus and A. lumbricoides) (Ct > 35). Four samples (9.1%) were low load of DNA (N. americanus) (Ct > 35) in post-treatment. Five samples (11.4%) were moderate load of DNA (A. lumbricoides) (30 < Ct < 35) in post-treatment. real-time PCR could detect submicroscopic infections from specific species of hookworm and A. lumbricoides.

Abstrak :
Introduction: Constant exposure to a variety of microorganisms in domestic environment plays an important role in the shaping of individual immune response mechanism, which can affect one's susceptibility to the diseases. The aim of the study to get an understanding how the exposure of microorganisms in the the different area where the people living might give a contribution to the profile and the regulation of the immune respons after stimulated to malaria, vaccine BCG and oxLDL antigents in PBMC and whole blood cultures, and to evaluate the character of T reg as a mediator to suppress the cell proliferation. Methode: It is an in vitro experimental study performed at Laboratorium Terpadu, Faculty of Medicine Univertas Indonesia, Jakarta in 2013 2014. As a model of infectious diseases is used pathogenic antigents such as Plasmodium falciparum infected red blood cells malaria and bacille calmette gu rin BCG vaccine, and as a modell of inflammatory disease is used non a patonegic antigen, low density lipoprotein LDL . Whole blood cultures is done for 80 blood samples to know how the regulation of immune respons from people living a rural populatin. PBMC cultures is also done to explore macrophages after stimulated to malaria, BCG and LDL. PHA stimulated to the PBMC culture with and without T reg cells to evaluate the character of T reg. T regulatory cells perhaps play the important roles to suppress the immune respons to microorganisms was also done. Results: The profile of the immune respons of the people living in the unslum area is significantly more inflamatif than that in the slum area. The ratio of pro anti inflammation cytokines TNF IL 10 of the people living in the unslum area is significantly higher than that in the slum area. This is marked by increasing of oxLDL accumulationis that is the important point of the low protection to oxLDL of the people living in the unslum area p 0.01 . T regulatory cell may suppress the proliferation in the PBMC culture for the people living in the slum area marked by increasing not only the expression of IL 10 cytokines but also the sum of T regulatory sells p 0.01 significantly. Conclusion: The immune respons of the people living in the unslum area is more inflamatif and responsive to malaria, BCG vaccine and oxLDL. The character of macrophage of the people living in the slum area is marked by the low ratio of pro anti inflammation cytokines TNF IL 10 to malaria, BCG vaccine and oxLDLstimulations. T regulatory cell may suppress the proliferation in the PBMC culture for the people living in the slum.