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Abstrak :
ABSTRAK
Latar Belakang: Streptococcus mutans (S.mutans) merupakan bakteri utama penyebab karies. Virulensi S.mutans dapat digambarkan melalui profil protein. Telah banyak penelitian yang menyebutkan propolis memiliki sifat anti bakteri dan dapat menurunkan prevalensi S.mutans. Tujuan: Mengetahui perbedaan efek antara permen propolis hisap, permen X dan permen madu terhadap profil protein S mutans yang diisolasi dari plak gigi. Metode: Sampel plak diambil sebelum dan sesudah perlakuan lalu dibiakkan pada medium agar dan medium cair TYS Broth. Profil protein dianalisis dengan metode SDS PAGE. Hasil: Permen X dan permen madu meningkatkan ekspresi protein GbpB, GbpC dan menurunkan ekspresi protein GtfB, GtfC, GtfD dan AgI/II. Permen propolis hisap dan permen madu sama-sama meningkatkan ekspresi protein GbpD. Kesimpulan: Permen X dan permen madu memiliki efek yang cukup mirip dalam mengubah profil protein S.mutans. Sementara permen propolis hisap memiliki efek berlawanan dengan permen X dan permen madu.

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ABSTRACT
Background: Streptococcus mutans (S.mutans) is the main bacteria that caused caries. S.mutans’ virulence can be observed by protein profile. There have been lots of researches which proved that propolis has antibacterial properties and can reduce the prevalence of S.mutans. Objectives: To know the differences between the effects of propolis candy, X candy, and honey candy to the protein profile of S.mutans that isolated from dental plaque. Methods: Plaque samples were taken before and after treatment and then cultured on agar and liquid medium TYS Broth. Protein profile was analyzed using SDS PAGE method. Result: X candy and honey candy both increase the protein expression of GbpB, GbpC and decrease the protein expression of GtfB, GtfC, GtfD and AgI/II. Propolis candy and honey candy both increase the protein expression of GbpD. Conclusion: X candy and honey candy have fairly similar effects in changing the protein profile of S.mutans. While, propolis candy has the opposite effects with X candy and honey candy.

Abstrak :
ABSTRAK Latar Belakang: Pengaruh Human Platelat Lysate (HPL) tanpa penambahan EGF dan bFGF dalam medium kultur HUVEC terhadap ekspresi CD45 belum diketahui sebelumnya. Tujuan: Mengevaluasi pengaruh penggunaan HPL tanpa penambahan EGF dan bFGF terhadap ekspresi CD45 pada HUVEC. Metode: HUVEC dikultur menggunakan FBS 2% dan HPL 2% dengan penambahan EGF dan bFGF serta HPL 2% dan HPL 5% tanpa penambahan EGF dan bFGF, kemudian ekspresi CD45 dianalisis dengan FACS. Hasil: Ekspresi CD45 pada HPL tanpa penambahan EGF dan bFGF tidak berbeda dibandingkan dengan FBS dan HPL dengan penambahan EGF dan bFGF. Simpulan: Penggunaan HPL tanpa penambahan EGF dan bFGF dalam medium kultur HUVEC tidak berpengaruh terhadap ekspresi CD45 pada HUVEC.

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ABSTRACT Background: Effect of HPL without additional EGF and bFGF in HUVEC culture medium on CD45 expression has not been studied previously. Objective: To evaluate the effect of HPL without additional EGF and bFGF on HUVEC CD45 expression. Method: HUVEC cultured with FBS 2%, HPL 2% with EGF and bFGF addition, HPL 2% and HPL 5% without EGF and bFGF addition, CD45 expression were then analysed with FACS. Result: CD45 expression of HUVEC cultured in HPL without additional EGF and bFGF were not different significantly compared to FBS and HPL with additional EGF and bFGF. Conclusions: The Use of HPL without additional EGF and bFGF in HUVEC culture medium did not affect HUVEC CD45 expression.

Abstrak :
ABSTRAK
Latar Belakang: Streptococcus mutans adalah bakteri penyebab karies dan memiliki faktor virulensi yang tinggi sehingga dapat memicu pembentukan biofilm pada gigi. Propolis merupakan bahan alami yang memiliki sifat antibakteri. Permen propolis hisap UI diduga dapat menghambat pembentukan biofilm bakteri Streptococcus mutans yang diisolasi dari plak gigi. Tujuan: Mengetahui efek permen propolis hisap UI terhadap pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi. Metode: Streptococcus mutans pada sampel plak subjek diambil sebelum dan setelah perlakuan. Sampel dikultur dalam medium TYS20B kemudian dilakukan kultur pada medium cair TYS Broth. Setelah itu dilakukan uji biofilm dengan crystal violet assay. Hasil: Hasil uji Wilcoxon menyimpulkan tidak terdapat perbedaan pembentukan biofilm Streptococcus mutans yang bermakna sebelum dan setelah konsumsi permen propolis (p=0,697) Kesimpulan: Setelah konsumsi selama tujuh hari, permen propolis tidak mempengaruhi pembentukan biofilm Streptococcus mutans yang diisolasi dari plak gigi.

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ABSTRACT
Background: Streptococcus mutans is one of the etiology of caries with high virulence factors which can improve its ability to form biofilm on dental surface. Propolis is a natural product which has antibacterial properties. Propolis candy made by University of Indonesia was expected to decrease the ability of Streptococcus mutans to form biofilm. Aim: To know the effect of propolis candy on biofilm formation ability of Streptococcus mutans isolated from dental plaque. Method: Streptococcus mutans specimen from dental plaque of 120 subjects were collected pre and post treatment. Plaque sample then cultured on agar medium TYS20B and then cultured on TYS Broth medium. Then, biofilm formation of Streptococcus mutans from sample evaluated by crystal violet assay. Result: The results of Wilcoxon test conclude that there is no statistically significant difference between the biofilm formation of Streptococcus mutans before and after consumption of propolis candy (p=0,697) Conclusion: After seven days of consumption, propolis candy has no effect on biofilm formation of Streptococcus mutans isolated from dental plaque.