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"Penelitian bertujuan mengetahui keanekaragaman bakteri Ktedonobacteria dari sampel tanah hutan di sekitar Geiser Cisolok, Jawa Barat dengan metode culture-dependent dan metode culture-independent. Isolasi bakteri menggunakan medium Reasoner's 2A (10%) dengan penambahan 2% gellan gum, cycloheximide, dan sodium azide. Inkubasi dilakukan pada suhu 30 oC selama 3 minggu. Amplifikasi gen 16S rRNA isolat bakteri menggunakan primer spesifik Ktedonobacteria (primer 161F dan 941R), dan primer universal bakteri (9F dan 1510R). Identitas isolat bakteri diperoleh berdasarkan data full sequence gen 16S rRNA melalui pencarian homologi pada EZBioCloud (www.ezbiocloud.net). Analisis filogenetik menggunakan metode Neighbour Joining, Maximum Evolution, dan Maximum Likelihood. Analisis keanekaragaman bakteri Ktedonobacteria menggunakan Next Generation Sequencing berdasarkan data partial sequence (daerah variabel V1--V3) dari gen 16S rRNA. Analisis data komposisi taksonomi bakteri dan indeks keanekaragaman menggunakan software QIIME2. Empat isolat Ktedonobacteria dengan kode K17-1, K17-2, K42, dan K44 berhasil diperoleh. Analisis filogenetik menunjukkan bahwa keseluruhan isolat merupakan anggota kelas Ktedonobacteria dan berada dalam satu grup dengan type strain Dictyobacter aurantiacus S-27T. Namun demikian, persentase homologi sequence gen 16S rRNA keempat isolat menunjukkan nilai yang rendah terhadap type strain Dictyobacter aurantiacus S-27T, yaitu 97.16 -- 98.02%. Berdasarkan nilai tersebut, keempat isolat yang diperoleh diduga merupakan spesies baru. Hasil analisis dengan software QIIME2 menunjukkan bahwa sampel tanah yang digunakan memiliki nilai indeks keanekaragaman bakteri yang tinggi, dengan nilai sebagai berikut: 6,49 (Shannon-Winner); 0,98 (Simpson); 177 (Chao1); dan 117 (Ace). Filum Acidobacteria, Proteobacteria dan Bacteriodetes, merupakan tiga filum dengan persentase paling besar pada sampel tanah, dengan nilai persentase masing-masing 44%, 25%, dan 9%. Kelas Ktedonobacteria pada filum Chloroflexi memiliki persentase yang sangat rendah, yaitu 1,89%. Namun demikian, analisis filogenetik data amplikon (culture-independent) menunjukkan bahwa Ktedonobacteria yang terdapat pada sampel tanah tersebar dalam 5 grup, yang seluruhnya mengindikasikan taksa baru. Penelitian ini menunjukkan bahwa metode culture-dependent hanya berhasil menemukan satu dari lima grup Ktedonobacteria yang berhasil dideteksi menggunakan metode culture-independent.

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The study aims to determine the diversity of Ktedonobacteria from forest soil samples around the Cisolok Geiser, West Java with culture-dependent and culture-independent methods. Bacterial isolation using Reasoner's 2A (10%) medium with 2% gellan gum, cycloheximide, and sodium azide. Incubation was carried out at 30 oC for three weeks. Amplification of 16S rRNA gene of bacterial isolates performed using Ktedonobacteria specific primers (primers 161F and 941R), and universal bacterial primers (9F and 1510R). The identity of bacterial isolates was obtained based on full 16S rRNA gene sequence data through a homology search on EZBioCloud (www.ezbiocloud.net). The phylogenetic analysis was performed by Neighbor-Joining, Maximum Evolution, and Maximum Likelihood methods. Analysis of Ktedonobacteria diversity using Next-Generation Sequencing based on partial sequence data (variable regions V1 -- V3) of the 16S rRNA gene. Analysis of bacterial taxonomy composition data and diversity index was conducted using QIIME2 software. Four isolates of Ktedonobacteria, namely K17-1, K17-2, K42, and K44, were successfully obtained. Phylogenetic analysis showed that all isolates were members of the class Ktedonobacteria and were in the same group as Dictyobacter aurantiacus S-27T. However, the percentage of homology of the 16S rRNA gene sequence of the four isolates showed a low value on the type strain of Dictyobacter aurantiacus S-27T, which accounted for 97.16 -- 98.02%. Based on these values, the four isolates obtained probably belonged to the new species. The results of the analysis with QIIME2 software showed that the soil samples had high bacterial diversity index values, with the following values: 6,49 (Shannon-Winner); 0,98 (Simpson); 177 (Chao1); and 117 (Ace). Phylum Acidobacteria, Proteobacteria, and Bacteriodetes are the three phyla with the largest percentage in soil samples, with percentage values of 44%, 25%, and 9%, respectively. Whereas the class Ktedonobacteria in the phylum Chloroflexi has a very low percentage, which is 1.89%. However, phylogenetic analysis of the amplicon data (culture-independent) showed that Ktedonobacteria found in soil samples distributed into five groups, indicating new taxa. In this study, culture-dependent methods found only one of the five groups of Ktedonobacteria that detected using the culture-independent method."

"Penelitian ini bertujuan untuk memperoleh informasi mengenai pengaruh variasi mediumpertumbuhan terhadap pembentukan miselium aerial dan aktivitas antimikroba delapanisolat rare Actinobacteria termofilik dari tanah di sekitar geiser Cisolok, Jawa Barat.Pengujian pertumbuhan, pembentukan miselium aerial, dan aktivitas antimikrobadilakukan dengan menumbuhkan isolat pada medium ISP 1 agar, ISP 1 gellan gum, ISP2 agar, ISP 2 gellan gum, ISP 3 agar, ISP 3 gellan gum, Bennett’s agar, Bennett’s gellangum, Minimal (Mm) 3 agar, Mm 3 gellan gum, 2% agar, dan 2% gellan gum. Isolatkemudian diinkubasi pada suhu 45 °C selama 7 dan 14 hari. Konfirmasi suhupertumbuhan menunjukkan 2 isolat dapat tumbuh hingga suhu 45 °C dan 6 isolat dapattumbuh hingga 50 °C. Hasil pengujian variasi medium pertumbuhan menunjukkan semuaisolat rare Actinobacteria dapat menghasilkan miselium substrat pada semua medium.Hasil pengamatan setelah inkubasi selama 7 hari pada suhu inkubasi 45 °C menunjukkanisolat-isolat tersebut dapat menghasilkan miselium aerial pada medium ISP 1 agar (2isolat), Mm 3 agar (3 isolat), 2% agar (5 isolat), dan 2% gellan gum (5 isolat). Hasilpengamatan setelah inkubasi selama 14 hari menunjukkan isolat-isolat tersebut dapatmenghasilkan miselium aerial pada medium ISP 1 gellan gum (2 isolat), ISP 2 agar (1isolat), ISP 2 gellan gum (3 isolat), ISP 3 agar dan gellan gum (2 isolat), Mm 3 agar 3isolat, dan Mm 3 gellan gum (3 isolat). Hasil pengujian aktivitas antibakteri menunjukkanisolat SL3-2-R-11 yang ditumbuhkan pada medium ISP 3 gellan gum dan SL3-1-R-7yang ditumbuhkan pada Bennett’s agar selama 7 hari dapat menghambat pertumbuhanStaphylococcus aureus. Hasil pengujian aktivitas antikhamir menunjukkan isolat SL3-2-R-11 yang ditumbuhkan pada medium ISP 3 gellan gum dan SL3-1-R-7 pada mediumBennett’s agar selama 14 hari dapat menghambat pertumbuhan Candida albicans. Hasilpengujian aktivitas antifungi menunjukkan tidak ada isolat yang dapat menghambatpertumbuhan Aspergillus flavus.

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This study aims to obtain information about the effect of growth medium variations onthe formation of aerial mycelium and antimicrobial activity of eight thermophilic rareActinobacteria isolates from the soil around Cisolok geyser, West Java. The ability togrow at various media, aerial mycelium formation, and antimicrobial activity were carriedout by growing isolates on medium ISP 1 agar, ISP 1 gellan gum, ISP 2 agar, ISP 2 gellangum, ISP 3 agar, ISP 3 gellan gum, Bennett’s agar, Bennett’s gellan gum, Minimum (Mm)3 agar, Mm 3 gellan gum, 2% agar, and 2% gellan gum. The isolates were then incubatedat 45 oC for 7 and 14 days. Growth test at various temperatures showed that two isolatescould grow at a temperature of 45 oC and six isolates could grow up to 50 oC. The resultsof the growth medium variation test showed that all rare Actinobacteria isolates couldproduce substrate mycelium in all mediums. Observations after incubation for 7 days at45 °C showed that these isolates could produce aerial mycelium on ISP 1 agar medium(2 isolates), Mm 3 agar (3 isolates), 2% agar (5 isolates), and 2% gellan gum (5 isolates).Observations after incubation for 14 days showed that these isolates could produce aerialmycelium on the medium ISP 1 gellan gum (2 isolates), ISP 2 agar (1 isolate), ISP 2gellan gum (3 isolates), ISP 3 agar and gellan gum (2 isolates), Mm 3 agar 3 isolates, andMm 3 gellan gum (3 isolates). The results of antibacterial activity test showed that isolatesSL3-2-R-11 grown on ISP 3 gellan gum and SL3-1-R-7 grown on Bennett’s agar for 7days could inhibit the growth of Staphylococcus aureus. The antifungal activity test ofisolates SL3-2-R-11 grown on ISP 3 gellan gum medium and SL3-1-R-7 on Bennett’sagar for 14 days showed inhibition towards Candida albicans. Meanwhile, all isolates didnot show antifungal activity against Aspergillus flavus"

"ABSTRAKTelah dilakukan penelitian yang bertujuan untuk menginduksi responspertahanan tanaman tembakau oleh lipopolisaakrida (LPS). LPS diekstraksi daribakteri Pseudomonas syringae pv. tabaci (Pta) dan P. syringae pv. glycinea (Pgl).Respons pertahanan tanaman yang diamati adalah deposisi callose dan ekspresigen terkait pertahanan (PAL, HIN 1 dan HSR 203J). Untuk pengamatan deposisicallose, daun tembakau diinfiltrasi dengan LPS Pta dan Pgl (400 µg/ml dan 800µg/ml) serta diinkubasi selama 24 dan 48 jam. Selanjutnya, klorofil daundiluruhkan menggunakan larutan laktofenol dan diwarnai dengan aniline blue.Deposisi callose diamati dibawah mikroskop fluoresensi. Hasil pengamatanmenunjukkan LPS bakteri Pgl menginduksi deposisi callose lebih banyakdibandingkan LPS bakteri Pta. Pengamatan ekspresi gen-gen terkait pertahanandilakukan pada daun tembakau yang diinfiltrasi dengan 400 µg/ml LPS bakteriPta and Pgl, serta diinkubasi selama 6 jam. Hasil RT-PCR terhadap dauntembakau menunjukkan LPS bakteri Pta dan Pgl mampu menginduksi ekspsresigen HIN 1, tetapi tidak mampu menginduksi ekspresi gen PAL dan HSR 203J.Gen HIN 1 terekspresi lebih kuat pada daun tembakau yang diinduksi oleh LPSbakteri Pgl daripada LPS Pta. Hasil penelitian mengindikasikan bahwa LPSbakteri Pgl menginduksi respons pertahanan daun tembakau lebih baik daripadaLPS bakteri Pta.

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AbstractThe aim of this study is to know the induction of tobacco defenseresponses by using lipopolysaccharides (LPS) which extracted from twophytopathogen, Pseudomonas syringae pv. tabaci (Pta) and P. syringae pv.glycinea (Pgl). The plant defense responses that observed are callose depositionand expression of defense-related genes (PAL, HIN 1 and HSR 203J). To detectcallose deposition, tobacco leaves were infiltrated with 400 µg/ml and 800 µg/mlLPS Pta and Pgl, then incubated for 24 or 48 hr. Tobacco leaves were cleared inlactophenol solution, stained with aniline blue, then visualized by fluorescencemicroscopy. The result showed that LPS from Pgl induced more callosedeposition than that from Pta in tobacco leaves. To investigate defense-relatedgenes expression, tobacco leaves were infiltrated with 400 µg/ml LPS extractedfrom Pta and Pgl, then incubated for 6 hr. Analysis of defense-related genesexpression were conducted by RT-PCR and visualized by electrophoresis on a1.8% agarose gel. The result showed LPS Pta and Pgl can induce expression ofHIN 1 gene in tobacco leaves, but can not induce the PAL and HSR 203J genes.The HIN 1 gene was highly expressed in tobacco leaves induced by LPS Pgl. Theresult indicates that tobacco could effectively recognize LPS of nonhost pathogenPgl but not in host pathogen Pta."

"Penapisan amilase 32 isolat actinomycetes dari sedimen pesisir dan serasah lamun Pulau Pramuka, Kepulauan Seribu, DKI Jakarta dilakukan secara kualitatif dan semi-kuantitatif menggunakan metode iodin. Penapisan amilase dari isolat secara kualitatif pada medium starch agar dilakukan dengan mengukur lebar clear zone dan diekspresikan dalam nilai indeks aktivitas. Penapisan amilase secara semi-kuantitatif pada medium pertumbuhan starch broth dilakukan dengan spektrofotometer pada panjang gelombang cahaya 620 nm. Pengukuran kadar glukosa yang terbentuk dan aktivitas amilase dari isolat terpilih dalam medium soluble starch 1% (SBs) dan tepung beras 1% (SBb) dilakukan dengan metode Dinitrosalicylic Acid (DNS) pada panjang gelombang 540 nm. Hasil penapisan amilase kualitatif menunjukkan indeks aktivitas amilase tertinggi diperoleh dari isolat SD 5 (4,259), SD 13 (4,154), SD 14 (3,457), dan SD 15 (4,436). Sementara itu, hasil penapisan amilase semi-kuantitatif menunjukkan isolat SD 13 memiliki transmitansi tertinggi dengan nilai 64,3% dan aktivitas amilase diukur lebih lanjut dengan metode DNS. Hasil uji kadar glukosa menunjukkan bahwa isolat SD 13 dalam SBs menghasilkan kadar glukosa yang lebih tinggi (6.379,345 μg/mL) dibandingkan dengan isolat SD 13 SBb (3.254,741 μg/mL). Aktivitas amilase SD 13 pada medium SBs lebih tinggi (26,124 ± U/mL) dibandingkan dengan aktivitas amilase pada medium SBb (14,205 U/mL). Tingginya aktivitas amilase pada SBs tersebut setara dengan banyaknya kadar glukosa yang terbentuk. Isolat SD 13 teridentifikasi secara molekuler berdasarkan data sekuens gen 16S rRNA sebagai anggota dari spesies Streptomyces champavati.

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Amylase activity of 32 Actinomycetes isolates from coastal sediment and seagrass litter of Pramuka Island, Kepulauan Seribu, DKI Jakarta were screened using two different methods involving iodine in starch medium. The first amylase screening test using starch agar was performed by measuring width of the clear zone and was expressed in activity index value. The second amylase screening test used starch broth for was done by using spectrophotometer in 620 nm of wave length.The first result showed amylase activity index is highest in SD 5 (4.259), SD 13 (4.154), SD 14 (3.457), and SD 15 (4.436) respectively. The second result indicated SD 13 has the highest transmittance of 64,3% and its glucose concentration and amylase activity was further measured using Dinitrosalisylic acid (DNS) method with two different media; 1% soluble starch (SBs) dan 1% rice flour (SBb) in 540 nm of wave length. The result showed that SD 13 isolate in SBs medium has higher glucose concentration (6,379.345 μg/mL) than in SBb (3,254.741 μg/mL). Amylase activity assay result indicated that SD 13 in SBs produced amylase with 26.124 of Enzyme Unit (U/mL), higher than in SBb (14.205 U/mL). The result was supported by the previous glucose concentration measurement. Molecular identification based on 16S rRNA gene sequences showed that SD 13 belongs to Streptomyces champavati."

"Bakteri pendegradasi naftalena dapat diisolasi dari lumpur vulkanik yang kaya akan organik bahan seperti hidrokarbon aromatik. Tujuan dari penelitian ini adalah untuk mengisolasi isolat bakteri yang mampu menurunkan naftalena dan mengkarakterisasi penggunaan isolat metode fenotipik dan molekuler. Isolasi isolat bakteri dilakukan dengan menggunakan a metode penyebaran setelah melalui teknik pengayaan di Bushnell-Haas (BH) sedang ditambah dengan bahan bakar diesel 1% (w / v). Bakteri isolat DRK 9.1 diperoleh dari sampel lumpur di Desa Renokenongo, Sidoarjo. Isolat ditanam di Bushnell- Haas medium dengan penambahan ekstrak ragi 0,5% (b / v) dan naphthalene 0,02% (b / v). Penambahan ekstrak ragi diyakini mampu meningkatkan pertumbuhan hidrokarbon mendegradasi bakteri dan degradasi hidrokarbon. Pencacahan dilakukan dengan menggunakan Metode Jumlah Lempeng Total dan degradasi naftalena ditentukan kuantitatif dengan Kromatografi Cair Kinerja Tinggi (HPLC). Hasilnya terungkap isolat DRK 9.1 mampu tumbuh dalam medium dengan penambahan ekstrak ragi 0,5% (b / v) dan 0,02% naftalena (b / v), dengan jumlah sel total meningkat setelah 24 jam dari 2,71 x 109 menjadi 5,61 x 1010 CFU / mL dan menurun setelah 96 jam menjadi 2,83 x1010 CFU / mL. Hasil analisis HPLC mengungkapkan bahwa isolat bakteri DRK 9.1 bisa menurunkan naphthalene hingga 70,42% setelah 24 inkubasi dan 99,59% setelah 96 jam inkubasi. Karakterisasi bakteri dilakukan dengan pengamatan fenotipik, uji biokimia, dan metode molekuler menggunakan gen 16S-rRNA. Hasil dari fenotipik dan karakterisasi biokimia menunjukkan bahwa isolat DRK 9.1 memiliki karakteristik dari bakteri yang menjadi anggota genus Pseudomonas. Isolat menunjukkan Kemiripan 99,64% dengan Pseudomonas aeruginosa JCM5962.

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Naphthalene degrading bacteria can be isolated from volcanic mud that is rich in organic materials such as aromatic hydrocarbons. The purpose of this study was to isolate bacterial isolates capable of reducing naphthalene and characterize the use of phenotypic and molecular isolates. Isolation of bacterial isolates was carried out using the spread method after the enrichment technique in Bushnell-Haas (BH) was being added with 1% (w / v) diesel fuel. DRK 9.1 isolate bacteria were obtained from mud samples in Renokenongo Village, Sidoarjo. Isolates were grown in Bushnell-Haas medium with yeast extract 0.5% (w / v) and naphthalene 0.02% (w / v). The addition of yeast extract is needed to increase hydrocarbon growth degrade bacteria and degradation of hydrocarbons. Enumeration was carried out using the Quantity Plate Method and the degradation was determined quantitatively by High Performance Liquid Chromatography (HPLC).

It was revealed that DRK 9.1 isolate was able to grow in the medium by requiring yeast extract 0.5% (w / v) and 0.02% naphthalene (w / v), with the total cell count increasing after 24 hours from 2.71 x 109 to 5 .61 x 1010 CFU / mL and decreases after 96 hours to 2.83 x1010 CFU / mL. The HPLC analysis results revealed 9.1 DRK bacterial isolates can reduce naphthalene to 70.42% after 24 incubations and 99.59% after 96 hours of incubation. Bacterial characterization was carried out by phenotypic experiments, biochemical tests, and molecular methods using the 16S-rRNA gene. The results of phenotypic and biochemical characterization showed that DRK 9.1 isolate had characteristics of bacteria that belong to the genus Pseudomonas. Isolates showed 99.64% Similarity with Pseudomonas aeruginosa JCM5962."

"Naphthalene adalah polutanik aromatik hidrokarbon (PAH) polutan hadir di lingkungan Hidup. Penghapusan naftalena dapat dicapai dengan biodegradasi menggunakan bakteri. Lumpur vulkanik mengandung bahan organik termasuk PAH, dan dapat mendukung pertumbuhan bakteri pendegradasi hidrokarbon. Strain bakteri Pseudomonas aeruginosa DRK 9.1 adalah diisolasi dari Lumpur Vulkanik di Desa Renokenongo, Sidoarjo. Tujuannya Penelitian adalah untuk menentukan kemampuan Pseudomonas aeruginosa DRK 9.1 untuk terdegradasi naftalena dengan penambahan glukosa sebagai stimulan pertumbuhan. Pseudomonas aeruginosa DRK 9.1 ditanam di media Bushnell-Haas dengan penambahan naphthalene 0,02% (b / v) dan glukosa 0,5% (b / v). Enumerasi sel bakteri dilakukan menggunakan Total Plate Count (TPC) dan degradasi naftalena ditentukan secara kuantitatif menggunakan Kromatografi Cair Kinerja Tinggi (HPLC). Hasilnya menunjukkan bahwa sel jumlah total meningkat dari 6,18 x 109 CFU / mL menjadi 2,10 x 1011 CFU / mL setelah 24 jam inkubasi. Setelah 96 jam inkubasi, jumlah sel menurun menjadi 3,32 x 1010 CFU / mL. Hasil biodegradasi naftalena oleh HPLC menunjukkan konsentrasi naftalena menurun masing-masing sebesar 40,85% dan 82,47% setelah inkubasi 24 dan 96 jam.

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Naphthalene is an aromatic hydrocarbon pollutant (PAH) pollutant present in the environment. Elimination of naphthalene can be achieved by biodegradation using bacteria. Volcanic mud contains organic matter including PAH, and can support the growth of hydrocarbon degrading bacteria. The Pseudomonas aeruginosa DRK 9.1 bacterial strain was isolated from Volcanic Mud in Renokenongo Village, Sidoarjo.

The research objective is to determine the ability of Pseudomonas aeruginosa DRK 9.1 to degrade naphthalene by adding glucose as a growth stimulant. Pseudomonas aeruginosa DRK 9.1 was planted in Bushnell-Haas media with the addition of 0.02% (w / v) naphthalene and 0.5% (w / v) glucose. Enumeration of bacterial cells was carried out using Total Plate Count (TPC) and naphthalene degradation was determined quantitatively using High Performance Liquid Chromatography (HPLC).

The results showed that the total cell count increased from 6.18 x 109 CFU / mL to 2.10 x 1011 CFU / mL after 24 hours of incubation. After 96 hours of incubation, the number of cells decreased to 3.32 x 1010 CFU / mL. The results of naphthalene biodegradation by HPLC showed that naphthalene concentrations decreased by 40.85% and 82.47% after 24 and 96 hours incubation, respectively."

"Penelitian karakterisasi produk gen sintetik lipase Thermomyces lanuginosus yang diekspresikan oleh Bacillus subtilis DB104 rekombinan K7 bertujuan untuk mengetahui pengaruh suhu, pH, dan ion logam terhadap aktivitas lipase. Bacillus subtilis DB104 promXynAQ1 non rekombinan digunakan sebagai kontrol. Lipase rekombinan optimal diproduksi pada media LB yang mengandung substrat minyak zaitun 1% selama 24 jam. Aktivitas lipase rekombinan diuji pada berbagai variasi perlakuan suhu (40°C--80°C), pH (5--10), dan penambahan ion logam menggunakan metode uji aktivitas spektrofotometri p-nitrofenil palmitat (pNPP assay). Data aktivitas spesifik lipase rekombinan dianalisis menggunakan data standar deviasi. Hasil penelitian menunjukkan bahwa lipase rekombinan aktif maksimal pada suhu 80°C dan optimal pH 8 dengan aktivitas spesifik sebesar 1,488 U/mg. Penambahan ion logam Ca2+, Mg2+, Cu2+, dan senyawa pengelat EDTA berpengaruh menghambat aktivitas enzim lipase rekombinan.

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The research of characterization of lipase Thermomyces lanuginosus synthetic gene product expressed by recombinant Bacillus subtilis DB104 had been conducted to investigate the effects of temperature, pH, and metal ions toward the enzymatic activity. Non recombinant lipase of Bacillus subtilis DB104 promXynAQ1 was used as control. Recombinant lipase was optimally produced using LB media containing 1% olive oil during 24 hours incubation time. Recombinant lipase was assayed in various treatments of temperature (40°C--80°C), pH (5--10), and metal ion addition using spectrophotometric method of p-nitrophenyl palmitate assay (pNPP assay). Specific activity of recombinant lipase data were analyzed with deviation standard. Experiment results showed that activity of recombinant lipase is maximum at temperature 80°C and optimum at pH 8 in the amount of 1,488 U/mg. The presence of metal cations Ca2+, Mg2+, Cu2+, and chelating-agent EDTA gave an inhibitory effect on recombinant lipase activity."

"Standar Kementerian Kesehatan menyatakan bahwa setiap makanan dan minuman tidak boleh mengandung Escherichia coli. Tujuan penelitian adalah menentukan kualitas mikrobiologis dari makanan siap saji dan minuman yang dijajakan di kantin kampus FMIPA UI Depok. Sebanyak 15 sampel, terdiri dari 10 jenis makanan siap saji, empat jus yang berbeda, dan air keran diperkaya dalam medium Buffered Peptone Water BPW sebelum diuji koliform. Uji koliform dari setiap sampel dilakukan pada medium kromogenik Chromocult Coliform Agar - Enhanced Selectivity CCA - ES dan Harlequin E. coli Coliform Agar HEC dan medium fluorogenik Readycult Coliform 100 RC 100 pada suhu 37oC. Hasil menunjukkan bahwa semua sampel mengandung bakteri koliform non - E. coli dan 12 di antaranya mengandung Escherichia coli. Isolasi dari sampel memperoleh 12 strain E. coli dan 15 isolat koliform non-E. coli. Uji koliform fekal dilakukan dengan menggunakan medium RC 100 pada suhu 44,5oC serta diperkuat dengan uji indol menggunakan reagen Kovac rsquo;s. Hasil uji menunjukkan bahwa E. coli yang terdapat pada 12 sampel berasal dari fekal. Hanya 7 dari 15 isolat koliform non - E. coli merupakan koliform fekal non - E. coli. Berdasarkan hasil penelitian yang diperoleh dapat disimpulkan bahwa kualitas mikrobiologis dari makanan dan minuman siap saji kantin FMIPA UI tidak memenuhi standar dari Kementerian Kesehatan.

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The standards from the Ministry of Health state that food and drinks should be free of Escherichia coli. The aim of this research was to assess the microbiological quality of ready to eat foods and drinks that were offered at a canteen in the Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok. Fifteen samples comprising of 10 different foods, four different juices, and tap water were enriched in buffered peptone water BPW before tested for the presence of coliforms using two chromogenic media Chromocult Coliform Agar Enhanced Selectivity CCA ES and Harlequin E. coli agar HEC and a fluorogenic medium Readycult Coliform 100 RC 100 at 37oC. Results showed that all samples contained non E. coli coliforms and 12 of them contained Escherichia coli. Twelve E. coli strains and 15 non E. coli coliform isolates were isolated. Fecal coliform tests were conducted for the E. coli strains and coliform isolates by performing a coliform test with Readycult coliform 100 at 44.5oC. The tests were strengthened with an indole test that uses a Kovac rsquo s reagent. The tests showed that the isolated E. coli from 12 samples were of fecal origin while only 7 out of 15 coliform isolates were fecal non E. coli coliforms. It was concluded that the microbiological quality of the canteen rsquo s ready to eat food and drinks did not fulfill the standards from the Ministry of Health. "