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Zahra Savira
Abstrak :
Latar Belakang: Celah bibir dan palatum (CLP) merupakan salah satu kelainan kongenital yang menghasilkan defek jaringan lunak maupun jaringan keras dan membutuhkan perawatan rekonstruksi tulang alveolar dan palatum. Celah bibir dan palatum dianggap berasal dari anomali proliferasi sel akibat faktor genetika. Autologous bone graft adalah baku emas untuk memperbaiki defek tulang palatum pada pasien CLP. Namun demikian, perawatan tersebut membutuhkan prosedur yang invasif. Perawatan melalui rekayasa jaringan dapat menjadi alternatif perawatan. Rekonstruksi tulang alveolar melalui rekayasa jaringan membutuhkan jumlah sel yang banyak sehingga kapasitas proliferasi sel punca merupakan aspek penting dalam penerapan klinis. Sel punca pulpa gigi sulung (SHED) dan sel punca pulpa gigi permanen (DPSCs) dapat menjadi sumber sel yang ideal karena memiliki kapasitas proliferasi yang tinggi, kemampuan diferensiasi ke berbagai tipe sel, isolasi yang mudah, dan aksesibilitas yang baik. Namun, kapasitas proliferasi SHED dan DPSCs pasien CLP belum diketahui. Tujuan: Penelitian ini bertujuan membandingkan kapasitas proliferasi SHED dan DPSCs pasien celah bibir dan palatum. Metode: SHED dan DPSCs dari pasien CLP dikultur hingga mencapai 70%-80% confluent. Kapasitas proliferasi sel setelah dikultur selama 24 jam, 48 jam, dan 72 jam dianalisis melalui uji MTT. Hasil: SHED setelah dikultur 24 jam menunjukkan nilai rata-rata optical density yang lebih tinggi secara signifikan (p<0,05). SHED dan DPSCs setelah dikultur 48 jam dan 72 jam tidak menunjukkan perbedaan nilai rata-rata optical density secara statistik (p>0,05). Kesimpulan: SHED pasien CLP memiliki kapasitas proliferasi lebih tinggi secara signifikan hanya pada 24 jam pertama. Pada 48 jam dan 72 jam pertama, SHED dan DPSCs pasien CLP memiliki kesamaan kapasitas proliferasi. ......Background: Cleft lip and palate (CLP) is one of orofacial congenital malformations that results in both soft tissue and hard tissue defect. It requires reconstruction of the maxillary alveolar cleft. Cleft lip and palate is thought to be came from anomalies of cell proliferation caused by genetic factors. Autologous bone graft have been the gold standard treatment to repair maxillary alveolar and palate clefts. However, such treatment needs an invasive procedure that may induce pain. To overcome those disadvantages, tissue engineering has received attention to be new alternative treatment. Reconstruction of maxillary alveolar cleft requires huge number of stem cells so that proliferative capacity is important traits before clinical application. Stem Cells from Exfoliateed Deciduous Teeth (SHED) and Dental Pulp Stem Cells (DPSCs) can be ideal sources of stem cell since they are known to have high proliferative capacity, multilineage differentiation, ease of isolation, and well accesibility. However, proliferative capacity of SHED and DPSCs isolated from CLP patients have not yet known. Objective: The aim of this study was to compare proliferative capacity between cultured stem cells from exfoliated deciduous teeth and dental pulp stem cells isolated from cleft lip and palate patients. Methods: SHED and DPSCs isolated from cleft patient were cultured until it reached 70%-80% confluency. Proliferative capacity after culturing for 24 hours, 48 hours, and 72 hours were analyzed using MTT Assay. Results: SHED after culturing for 24 hours showed higher optical density average value significantly (p<0,05). SHED and DPSCs after culturing for 48 hours and 72 hours has no difference optical density average value significantly (p>0,05). Conclusions: SHED from cleft patients showed higher proliferative capacity significantly only on first 24 hours culturing. SHED and DPSCs have similar proliferative capacity on 48 hous and 72 hours culturing.
Depok: Fakultas Kedokteran Gigi Universitas Indonesia, 2019
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Azhar Satrio Wibisono
Abstrak :
ABSTRAK Latar Belakang : Bernapas melalui mulut merupakan upaya adaptasi untuk memenuhi kebutuhan udara. Kebiasaan ini dapat mengubah kondisi biologis di dalam lingkungan rongga mulut serta perkembangan anak-anak. Kondisi tersebut mempengaruhi kebersihan rongga mulut yang dapat menimbulkan bau mulut. Pengukuran kondisi bau mulut dapat diukur menggunakan metode organoleptik dengan indra. Enterococcus faecalis merupakan bakteri transien rongga mulut yang dapat ditemukan terutama pada saluran akar yang mengalami kegagalan perawatan endodontik. Penelitian mengenai keberadaan Enterococus faecalis pada anak-anak belum diketahui. Tujuan : Menganalisis keberadaaan Enteroccocus faecalis pada sampel saliva dan plak gigi anak-anak berdasarkan kelompok skor organoleptik dan OHI-S (Oral Hygiene Index-Simplified). Metode : Sampel saliva dan plak gigi anak usia 8-11 tahun diuji menggunakan metode ELISA (Enzyme-linked immunosorbent assay), kemudian dikelompokkan berdasarkan nilai organoleptik dan OHIS. Pengelolaan data dilakukan dengan membandingkan nilai antar kelompok anak-anak memiliki kecenderungan bernapas melalui mulut dengan tidak melalui mulut (bernafas melalui hidung). Hasil : Sebagian besar tidak ditemukan perbedaan bermakna antara kelompok anak-anak memiliki kecenderungan bernapas melalui mulut dan hidung berdasarkan pembagian nilai organoleptik dan OHI-S. Pada salah satu uji ditemukan terdapat perbedaan bermakna pada kelompok bernapas melalui hidung berdasarkan nilai organoleptik. Terdapat kecenderungan keberadaan antigen Enterococcus faecalis lebih tinggi pada plak gigi daripada saliva. Kesimpulan : Keberadaan antigen Enterococcus faecalis ditemukan lebih tinggi pada plak gigi dan terdapat kecenderungan keberadaan antigen Enteroccocus faecalis meningkat berkaitan dengan kondisi OHI-S.
ABSTRACT Background: Mouth breathing is a type of habitual adaptation of breathing to fulfill the needs of oxygen. This habit could alter the biological oral condition and development of children. The altered condition of the oral environment could affect oral hygiene and cause oral malodor. Organoleptic is using human sense as a measurement to assess severity of oral malodor. Enterococcus faecalis is the transient bacteria of the oral cavity particularly found in the root canal of the failed endodontic treatment teeth. Based on previous studies, Enterococcus faecalis existence in children is unknown. Purpose: To analyze the existence of Enterococcus faecalis antigen in salivary and tooth plaque samples of children based on organoleptic and OHI-S (Oral Hygiene Index-Simplified) score. Methods: Salivary and tooth plaque sample of children age 8-11 were tested with ELISA (Enzyme-linked immunosorbent assay) technique and divided into several groups. The grouping was done based on the organoleptic and OHI-S score of subjects. Data analyzed by comparing scores between children who have a tendency toward mouth breathing with those who breathe with nose based on their organoleptic and OHI-S score. Result: Mostly, there is no significant difference between groups who tend mouth breathing with those who breathe with nose based on organoleptic and OHI-S score. However, in one of the tests, there is significant difference within groups who breathe with nose based on organoleptic score. The antigen amount of Enterococcus faecalis was found higher in tooth plaque rather than in saliva. Conclusion: The amount of Enterococcus faecalis antigen is higher in tooth plaque and there is a tendency that the amount of Enterococcus faecalis is influenced by the OHI-S score.
Depok: Fakultas Kedokteran Univesitas Indonesia, 2019
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Yasmine Nuraida Safitri
Abstrak :
Pendahuluan: Periodontitis adalah penyebab utama kehilangan gigi pada orang dewasa dan dianggap sebagai masalah kesehatan masyarakat di seluruh dunia. Selain terapi mekanik, dengan dengan perawatan SRP (scaling dan root planing), antibiotik dalam bentuk gel juga diindikasikan sebagai perawatan periodontal. Terdapat beberapa pengembangan bahan alami sebagai pengganti perawatan periodontitis tersebut, salah satunya adalah gel propolis. Tujuan: Mengetahui efektivitas gel propolis 10% terhadap periodontitis pada model periodontitis Mus musculus dengan aplikasi ligature silk thread. Metode: 18 ekor Mus musculus dibagi menjadi kelompok perlakuan, kelompok kontrol positif, dan kelompok kontrol negatif (gel plasebo). Kemudian, periodontitis diinduksi dengan ligature silk thread 5.0 pada gigi molar dua maksila kanan. Pada hari ke-7 ligature dilepaskan, skor SBI (Sulcus Bleeding Index) dan kedalaman poket awal diamati, dan diapikasikan gel propolis 10% pada kelompok perlakuan, gel metronidazole pada kelompok kontrol positif pada poket periodontal, dan gel plasebo pada kelompok kontrol negatif. Pada hari ke-14 diamati skor SBI (Sulcus Bleeding Index) dan kedalaman poket akhir, dan dilakukan pengambilan sampel tulang alveolar untuk diamati dengan Stereomikroskop. Hasil: Tidak ada perbedaan bermakna pada skor SBI, kedalaman poket, dan luas kerusakan tulang antara kelompok perlakuan, kelompok kontrol positif, dan kelompok kontrol negatif. Kesimpulan: Gel propolis 10% tidak menunjukkan efek terapi terhadap periodontitis pada model periodontitis Mus musculus (Swiss Webster). ......Introduction: Periodontitis is the main cause of tooth loss on adults and considered a major problems of public health around the world. Antibiotics in gel form are used as an adjunctive treatment of standard periodontal therapy, scaling and root planning. There are developments of alternative therapy of periodontitis, and one of them is propolis. Objective: Discover efficacy of propolis gel 10% on periodontitis in ligature-induced Mus musculus periodontitis model. Methods: in vivo study on 18 Mus musculus, divided into intervention group, positive control group, and negative control group. Periodontitis is induced by placing ligature silk thread 5.0 around maxillary right second molar. On the seventh day, the ligature was removed, and the first SBI score and periodontal pocket depth were measured, before the application propolis gel 10% on intervention group, metronidazole gel on positive control group, and placebo gel on negative control group in periodontal pocket. On the fourteenth day, the last SBI score and periodontal pocket depth were measured, and the alveolar bone samples were taken to be observed by stereo microscope. Result: There are no significant difference of SBI score, periodontal pocket depth, and alveolar bone loss area between every group. Conclusion: Propolis gel 10% does not show any therapeutic effect on periodontitis in ligature-induced Mus musculus periodontitis model.
Depok: Fakultas Kedokteran Gigi Univeritas Indonesia, 2019
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Karla Monica Wijaya
Abstrak :
ABSTRAK
Latar Belakang: Propolis telah diketahui dapat mencegah terjadinya karies. Perlu penelitian lebih lanjut mengenai efektifitas antibakteri propolis, yaitu dengan pengujian aktivitas laktoperoksidase. Tujuan: Mengetahui efektifitas permen propolis madu yang dilihat dari aktivitas laktoperoksidase pada saliva tanpa stimulasi. Metode: Disain penelitian ini adalah eksperimental laboratorik yaitu pretest-posttest dan menggunakan sampel saliva yang diambil dari 120 subjek. Sampel saliva yang dikumpulkan kemudian ditambahkan dengan Kalium Iodida, Buffer fosfat, dan Hidrogen peroksida. Aktivitas laktoperoksidase ditunjukkan dengan nilai absorbansi yang dihitung menggunakan microplate reader pada panjang gelombang 340 nm. Hasil: Uji statistik menunjukkan bahwa pengaruh permen propolis madu terhadap aktivitas laktoperoksidase tidak signifikan (p>0.05). Kesimpulan: Permen propolis madu memiliki kecendrungan meningkatkan aktivitas laktoperoksidase, walaupun secara statistik tidak signifikan.
ABSTRACT
Background: Propolis is well known for preventing caries. Further study is needed to test the antibacterial effectivity of propolis through lactoperoxidase activity. Objective: This research was purposed to determine the effectivity of honey propolis hard candy which is shown by salivary lactoperoxidase activity in unstimulated saliva. Methods: This study design was pretest-posttest laboratory experimental design. This study used saliva which is collected from 120 subjects. Collected saliva is then reacted with Potassium Iodide, Phosphate Buffer, and Hydrogen Peroxide. Salivary lactoperoxidase activity is shown by absorbance value which is calculated by microplate reader at wavelength 340 nm. Result: Honey propolis hard candy increase salivary lactoperoxidase activity although statistically not significant (p>0.05). Conclusion: Honey propolis hard candy has tendency to increase salivary lactiperoxidase activity.
Fakultas Kedokteran Gigi Universitas Indonesia, 2014
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Ratu Annisa Septiani
Abstrak :
Latar belakang: Telah terbukti propolis memiliki sifat antibakteri. Pemanfaatan propolis yang sedang dikembangkan oleh Universitas Indonesia adalah permen propolis madu yang diduga dapat mempengaruhi aktivitas mieloperoksidase saliva. Tujuan: Mengetahui pengaruh konsumsi permen propolis madu terhadap aktivitas mieloperoksidase pada saliva tanpa stimulasi. Metode: Aktivitas mieloperoksidase dianalisis berdasarkan nilai absorbansi yang diukur menggunakan microplate reader dengan panjang gelombang 450 nm. Pengukuran aktivitas mieloperoksidase berdasarkan perubahan warna oksidasi DAB (3,3 diaminobenzidine) dan guaiacol. Hasil: Permen propolis madu memiliki kecenderungan meningkatkan aktivitas mieloperoksidase, namun secara statistik tidak signifikan. Kesimpulan: Efek permen propolis madu tidak mempengaruhi aktivitas mieloperoksidase, sehingga aktivitas mieloproksidase dalam saliva dapat bekerja optimal dan tidak mengganggu keseimbangan rongga mulut.
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2014
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Dewi Vasthi Manikmaya
Abstrak :
ABSTRAK
Latar Belakang: S.mutans merupakan salah satu mikroorganisme paling berpengaruh terhadap terjadinya karies gigi. Propolis, suatu zat resin yangdiproduksi oleh lebah memiliki aktivitas inhibitorik terhadap S.mutans. Tujuan: Menganalisis efek permen propolis madu terhadap profil protein S.mutans yang diisolasi dari saliva. Metode: Profil protein S.mutans dianalisis dengan metode SDS PAGE. Hasil: Setelah mengonsumsi permen X dan permen madu frekuensi ekspresi protein GbpA meningkat dan frekuensi ekspresi protein GtfB dan GbpB menurun. Setelah mengonsumsi permen propolis madu dan permen madu frekuensi ekspresi protein GbpC meningkat dan frekuensi ekspresi protein AgI/II dan GtfC menurun. Kesimpulan: Terjadi perubahan profil protein S.mutans setelah pengonsumsian permen propolis madu, permen X dan permen madu. Frekuensi ekspresi protein AgI/II, GtfC dan GbpA menurun sedangkan frekuensi ekspresi protein GtfB, GbpB, GbpC, GbpD meningkat setelah mengonsumsi permen propolis madu. Frekuensi ekspresi protein GtfB dan GbpB menurun sedangkan frekuensi ekspresi protein AgI/II, GtfD, GbpA, GbpC meningkat setelah mengonsumsi permen X. Frekuensi ekspresi protein AgI/II, GtfB, GtfC, GbpB, GbpC dan GbpD menurun sedangkan frekuensi ekspresi protein GbpA meningkat setelah mengonsumsi permen madu. Permen X dan pemen madu memiliki efek yang mirip dalam mengubah profil protein S.mutans. Sedangkan permen propolis madu memiliki efek yang berbeda.
ABSTRACT
Background: S.mutans is one of the most influential microorganism on dental caries development. Propolis, a resin produced by bees, has a strong inhibitoric activity on S.mutans growth. Objective: To analyze the effect of propolis honey candy to S.mutans protein profile isolated from saliva. Method: S.mutans protein profile was analyzed by SDS PAGE method. Results: After X candy and honey candy consumption, GbpA protein expression frequency increased, GbpB and GtfB protein expression frequency decreased. After propolis honey candy and honey candy consumption, GbpC protein expression frequency increased, AgI/II and GtfC protein expression frequency decreased. Conclussion: S.mutans protein profile was altered after propolis honey candy, X candy and honey candy consumption. AgI/II, GtfC, GbpA protein expression frequency decreased while GtfB, GbpB, GbpC, GbpD protein expression frequency increased after propolis honey candy consumption. GtfB, GbpB protein expression frequency decreased while AgI/II, GtfD, GbpA, GbpC protein expression frequency increased after X candy consumption. AgI/II, GtfB, GtfC, GbpB, GbpC, GbpD protein expression frequency decreased and GbpA protein expression frequency increased after honey candy consumption. X candy and honey candy have similar effect in altering S.mutans protein, while propolis honey candy has different effect.
Fakultas Kedokteran Gigi Universitas Indonesia, 2014
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Ivana Florentina
Abstrak :
[Udang kering adalah sumber daya alam Indonesia yang mudah diperoleh dan diduga mengandung kalsium yang cukup tinggi. Penelitian ini bertujuan untuk mengetahui kadar kalsium udang (Macrobrachium sp.) dan pengaruh metode preparasi (oven dan non oven) dan isolasi (pengocokan, digesti asam, dan pengabuan) terhadap pengukuran kadar kalsium menggunakan AAS dan ISE. Hasil penelitian menunjukkan kadar kalsium tertinggi diperoleh dengan metode isolasi digesti asam, yaitu 7.749 ppm (oven) dan 8.853 ppm (non oven). Terdapat perbedaan hasil pengukuran kalsium antar metode preparasi dan antar metode isolasi. Metode preparasi berkorelasi kuat dengan hasil pengukuran kalsium. (r2=0,878; p<0,05);Dried shrimps is one of Indonesia?s natural resources which easily obtained and assumed to contain high calcium. This research aims to know the calcium level in Macrobrachium sp. and the effects of preparation (oven and non oven) and isolation (dilution, acid digestion, and ashing) methods in calcium level measurement by AAS and ISE. Results showed that the highest calcium level was obtained by acid digestion isolation which are 7,749 ppm (oven) and 8,853 ppm (non oven). There were calcium level differences between preparation methods, and among isolation methods. Preparation methods have strong correlation with calcium level measurement. (r2=0.878, p<0.05), Dried shrimps is one of Indonesia’s natural resources which easily obtained and assumed to contain high calcium. This research aims to know the calcium level in Macrobrachium sp. and the effects of preparation (oven and non oven) and isolation (dilution, acid digestion, and ashing) methods in calcium level measurement by AAS and ISE. Results showed that the highest calcium level was obtained by acid digestion isolation which are 7,749 ppm (oven) and 8,853 ppm (non oven). There were calcium level differences between preparation methods, and among isolation methods. Preparation methods have strong correlation with calcium level measurement. (r2=0.878, p<0.05)]
[, Fakultas Kedokteran Gigi Universitas Indonesia], 2015
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Fenny Kamadi
Abstrak :
[Ebi merupakan udang kering berukuran kecil yang dapat dikonsumsi secara keseluruhan. Ebi diduga memiliki kadar kalsium tinggi meski belum ada penelitian spesifik pada genus tertentu. Penelitian ini bertujuan mengetahui kadar kalsium pada Metapenaeus sp. secara akurat serta menganalisis pengaruh metode preparasi (oven dan non oven) dan metode isolasi (pengocokan, digesti asam, dan pengabuan) terhadap pengukuran dengan ISE dan AAS. Hasil pengukuran didapatkan kadar kalsium tertinggi 6.769 ppm (oven) dan 7.785 ppm (non oven) dengan isolasi terbaik adalah digesti asam. Terdapat perbedaan serta korelasi kadar kalsium antar metode preparasi dan isolasi, sehingga disimpulkan kedua metode berpengaruh terhadap pengukuran kadar kalsium ebi.;Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have high calcium level even there has been no research on specific genus yet. This research aimed to know exact calcium level in Metapenaeus sp., analyze the influence of preparation (oven and non oven) and isolation (dilution, acid digestion, and dry ashing) methods toward ISE and AAS measurement. Results showed highest calcium level 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolation method. There were differences and correlation among preparation and isolation methods; and concluded both methods influenced calcium level measurement in ebi., Ebi is dried small sized shrimp consumed as a whole. Ebi is assumed to have high calcium level even there has been no research on specific genus yet. This research aimed to know exact calcium level in Metapenaeus sp., analyze the influence of preparation (oven and non oven) and isolation (dilution, acid digestion, and dry ashing) methods toward ISE and AAS measurement. Results showed highest calcium level 6,769ppm (oven) and 7,785ppm (non oven) with acid digestion as best isolation method. There were differences and correlation among preparation and isolation methods; and concluded both methods influenced calcium level measurement in ebi.]
[, Fakultas Kedokteran Gigi Universitas Indonesia], 2015
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Marpaung, Levy Jeremy
Abstrak :
Silver Diamine Fluoride SDF sudah terbukti sebagai cairan yang dijadikan sebagai agen antikaries. Namun Silver Diamine Fluoride dapat meninggalkan noda hitam pada permukaan gigi dimana Silver Diamine Fluoride tersebut diaplikasikan serta menghasilkan rasa metal. Propolis Fluoride adalah salah satu temuan untuk menggantikan Silver Diamine Fluoride sebagai agen antikaries tanpa memiliki efek samping yang sama. Tujuan: Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antimikrobial dari Propolis Fluoride dalam menghamabat pembentukan biofilm S.mutans dan dibandingkan dengan Silver Diamine Fluoride. Terdapat 3 variasi konsentrasi dari Propolis Fluoride yang diuji yaitu 3,3 Ekstrak Propolis 2,139 Fluoride , 6,3 Ekstrak Propolis 2,139 Fluoride , dan 10 Ekstrak Propolis 2,139 Fluoride. Metode: Suspensi S.mutans dikultur di dalam Brain Heart Infusion cair dan dan agar. Lalu suspensi diteteskan ke dalam 96-well plate dan dipaparkan dengan Propolis Fluoride lalu diinkubasi di dalam suasana anaerob selama 20 jam. Dilanjutkan dengan pemaparan kristal violet 0,5 pada 96-well plate dan pembacaan untuk mengukur besarnya Optical Density menggunakan microplate reader dengan gelombang 570 nm. Hasil: Potensi Propolis Fluoride 10 EP 2,139 Fluoride setara dengan potensi Silver Diamine Fluoride 38 . dalam menghambat pembentukan biofilm S.mutans. Hasil uji ANOVA dari Propolis Fluoride 10 EP 2,139 Fluoride p = 0,08. Kesimpulan: Potensi Propolis Fluoride dalam menghambat pembentukan biofilm S.mutans dapat disetarakan dengan potensi inhibisi Silver Diamine Fluoride 38. ......Background: Silver Diamine Fluoride is a liquid that has been proven to be an anti caries agent. But it leaves a black stain on the surface of the teeth where it was aplicated and it also have a metalic taste. Propolis Fluoride is one of the finding to replace Silver Diamine Fluoride as an anti caries agent without having the same side effect. Objective: The aim of this study is to evaluate the antimicrobial activity of Propolis Fluoride in inhibiting S.mutans biofilm formation in comparison to Silver Diamine Fluoride. There are three different variant of Propolis Fluoride concentration which is 3,3 Propolis Extract 2,139 Fluoride , 6,3 Propolis Extract 2,139 Fluoride , and 10 Propolis Extract 2,139 Fluoride. Methods: The suspension of S.mutans were cultured in Brain Heart Infusion broth and agar. Then the suspension was shed into 96 well plate and combined with Propolis Fluoride. Later, 96 well plate were incubated in anaerobic enviroment for 20 hour. Crystal violet 0,5 then shed into the 96 well plate. The amount of biofilm inhibition were evaluated using microplate reader in 570nm wavelength to find the amount of the Optical Density OD. Then the concentration were measure in OD then converted into percentage. Result: Propolis Fluoride 10 EP 2,139 Fluoride have the same potention in inhibiting biofilm formation of S.mutans as Silver Diamine Fluoride 38. The ANOVA result for the Propolis Fluoride 10 EP 2,139 Fluoride is p 0.08. Conclusion: The potention of Propolis Fluoride in inhibiting biofilm formation of S.mutans can be compared with Silver Diamine Fluoride.
Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2016
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Muhammad Irfan
Abstrak :
ABSTRAK
Saliva merupakan zat eksokrin yang mengandung berbagai komponen, salah satunya adalah protein. Total protein dalam saliva dapat meningkat karena peningkatan aktifitas fisik, salah satu contohnya adalah aktifitas berlari. Di dalam rongga mulut, Streptococcus mutans merupakan mikroorganisme kariogenik yang memiliki peran penting dalam proses terjadinya karies. Tujuan: menganalisis perbedaan profil protein saliva yang diisolasi dari subjek pelari dan nonpelari sebelum dan setelah protein tersebut diinteraksikan dengan Streptococcus mutans ATCC 25175. Metode: sampel saliva unstimulated diambil dari 3 subjek pelari dan 3 subjek nonpelari. Identifikasi berat molekul protein saliva ditetapkan dengan menggunakan teknik SDS-PAGE dan pewarnaan commasie blue, sedangkan identifikasi interaksi protein saliva dengan Streptococcus mutans ditetapkan dengan menggunakan teknik SDS-PAGE, pewarnaan commasie blue dan Qubit Protein assay. Hasil: pada subjek pelari teridentifikasi protein dengan berat molekul sebesar 140 kDa, 100 kDa, 70 kDa, 50 kDa, 25 kDa, dan 15 kDa sedangkan pada subjek nonpelari teridentifikasi protein dengan berat molekul 70 kDa, 50 kDa, 25 kDa, dan 10 kDa. Interaksi protein saliva pelari dengan metode pewarnaan comassie blue tidak memvisualisasikan pita pada agar poliakrilamid sedangkan protein saliva subjek nonpelari terlihat pita sebesar 70 kDa. Interaksi protein saliva pelari dengan Streptococcus mutans dengan menggunakan Qubit Protein assay menunjukan konsentrasi sebesar 74,2 g/mL dan sebesar 93,2 g/mL pada saliva nonpelari. Kesimpulan: terdapat perbedaan profil dan berat molekul protein saliva pada subjek pelari dan nonpelari dan interaksi protein dengan Streptococcus mutans hanya tervisualisasikan pada protein saliva yang berasal dari subjek nonpelari.
ABSTRAK
Background Saliva is an exocrine substance containing various components, one of which is protein. The amount of total proteins in saliva may increase due to physical activity, namely running. In the oral cavity, Streptococcus mutans is a cariogenic microorganism that is vital in the forming process of caries. Objective Analyze the difference in salivary proteins profiles on subjects who are runners compared to non runners before and after the proteins are interacted with Streptococcus mutans ATCC 25175. Method Samples of unstimulated saliva were taken from 3 subjects who were runners and 3 subjects who were non runners. Identification of total molecular weight in salivary proteins was done using comassie brilliant blue color staining with the SDS PAGE technique. Identification of interaction in salivary proteins with Streptococcus mutans was also done using comassie brilliant blue color staining using SDS PAGE technique and Qubit Protein assay. Results In subjects who were runners, identification of molecular weight in the salivary protein results were 140 kDa, 100 kDa, 70 kDa, 50 kDa, 25 kDa, and 15 kDa while in non runners the identification of molecular weight in the salivary protein results were 70 kDa, 50 kDa, 25 kDa, and 10 kDa. Interaction of salivary proteins in runners using comassie blue coloring did not result in visualization of band on polyacrilamide agar while in non runners a band of 70 kDa was observed on polyacrilamide agar. Interaction of salivary proteins with Streptococcus mutans using Qubit Protein assay showed a concentration of 74.2 g mL in runners and 93.2 g mL in non runners. Conclusion There is a difference of proteins profile and molecular weight in subjects who were runners and non runners and proteins interaction with Streptococcus mutans is only visualized in the salivary proteins derived from non runners subject.
2017
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