Deskripsi Lengkap
| Bahasa : | ind |
| Sumber Pengatalogan : | LibUI ind rda |
| Tipe Konten : | text (rdacontent) |
| Tipe Media : | unmediated (rdamedia); computer (rdamedia) |
| Tipe Carrier : | volume (rdacarrier); online resources (rdacarrier) |
| Deskripsi Fisik : | ix, 80 pages : illustration ; 28 cm + appendix |
| Naskah Ringkas : | |
| Lembaga Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI, Lantai 3 |
- Ketersediaan
- File Digital: 1
- Ulasan
- Sampul
- Abstrak
| No. Panggil | No. Barkod | Ketersediaan |
|---|---|---|
| S-Pdf | 14-19-497534710 | TERSEDIA |
| Tidak ada ulasan pada koleksi ini: 20176225 |
Abstrak
ABSTRAK
The existence of chioramphenicol residues in veterinary products could cause such a serious side effect like aplastic anemia. A specific and sensitive ELISA method to detect chioramphenicol residues was needed to be developed in order to get a more efficient and economical analysing method. In this study, a direct ELISA method has been done using chioramphenicol-bovine serum albumin (CAP-BSA) as antigen and antisera chioramphenicol labelled by enzyme horseradish peroxidase (HRP) as antibody. CAP-BSA with a dilution of 1:200 and antisera chioramphenicol labelled by }iRP with a dilution of 1:125 showed a limit detection of 0.05 ng/ml. CAP-BSA had been synthesized from chloramphenicol sodium succinate and BSA, using mixed anhydride reaction, whereas HRP-labelled antisera chloramphenicol had been synthesized from antisera chioramphenicol and FIRP, using periodate conjugation technique. The production of antisera chloramphenicol has been performed through CAP-BSA immunization using two New Zealand White rabbits resulting in antisera chloramphenicol with a less satisfactory specificity and sensitivity. The recovery assay of chloramphemcol in cow milk gave 98,17% ± 2,88% yield.
The existence of chioramphenicol residues in veterinary products could cause such a serious side effect like aplastic anemia. A specific and sensitive ELISA method to detect chioramphenicol residues was needed to be developed in order to get a more efficient and economical analysing method. In this study, a direct ELISA method has been done using chioramphenicol-bovine serum albumin (CAP-BSA) as antigen and antisera chioramphenicol labelled by enzyme horseradish peroxidase (HRP) as antibody. CAP-BSA with a dilution of 1:200 and antisera chioramphenicol labelled by }iRP with a dilution of 1:125 showed a limit detection of 0.05 ng/ml. CAP-BSA had been synthesized from chloramphenicol sodium succinate and BSA, using mixed anhydride reaction, whereas HRP-labelled antisera chloramphenicol had been synthesized from antisera chioramphenicol and FIRP, using periodate conjugation technique. The production of antisera chloramphenicol has been performed through CAP-BSA immunization using two New Zealand White rabbits resulting in antisera chloramphenicol with a less satisfactory specificity and sensitivity. The recovery assay of chloramphemcol in cow milk gave 98,17% ± 2,88% yield.