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Mannan is an oligo-saccharide that was used for human health as a prebiotic food and is producedfrom hydrolysis of palm kernel cake, coconut seed copra and coke waste. Domestically, palm kernelcake and copra utilized for low efficiency metabolized animal feed A t this moment, via glico-science andtechnology route, these farming waste using mannanase could be degraded to produce functional oligosaccharide such as mannose and manno-oligosaccharide. Around 20-40% of palm kernel cake fibrousfrom CPO waste consist beta-mannan. In the beginning, palm kernel cake fibrous qualitative andquantitative analysis and mannan preparation was done for the next enzymatic processing (fermentationstep). Optimum condition of palm kernel cake residue hydrolysis was 110°C used heating process till 1_5hour and 2% mass acid catalyst of 150 g/L, palm kernel cake residue solution. Here, hydrolysis 150 g/Lsubstrate solution at 90" C used heating process till 1.5 hour produced 19.1% mass mannan. Hydrolisisusing Streptomyces and Saccharopolyspora (strains from B TCC) resulted that both isolates have apotential to produce mannanase and this raw enzyme qualitativlyt capable to hydrolysis palm kernel cakefor producing oligo-saccharides. Both isolates produced mannanase which higher specific activity after24 hour inoculation of palm kernel cake residue solution. |
