Kapasitas ekstrak Temulawak (Curcuma xanthorrhiza Roxb) sebagai anti Streptococcus mutans dalam menghambat demineralisasi email (in vitro)
Handoko Tirta;
Ria Puspitawati, supervisor; Harun Asyiq Gunawan, supervisor; Widurini D.S., examiner; Lisa Rinanda Amir, examiner; Andi Soufyan Santosa, examiner
(Fakultas Kedokteran Gigi Universitas Indonesia, 2012)
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Xanthorrhizol yang di isolasi dari Temulawak dapat mempertahankan pH model biofilm in vitro selama 4 jam. Diketahui KBM ekstrak Temulawak terhadap S. mutans 25%. Tujuan : Menganalisis efek ekstrak Temulawak 25% terhadap demineralisasi email yang terpapar biofilm S.mutans. Metode : Model biofilm diperoleh dengan mengkultur S.mutans yang sudah ditanam dalam TYS broth selama 24 jam pada 6 well ?plate yang telah dilapisi pelikel. Ekstrak Temulawak dipaparkan pada model biofilm pada berbagai durasi antara 1-48 jam. Pengukuran pH menggunakan pH universal indicator. Model biofilm juga ditumbuhkan pada permukaan sample gigi. Pemaparan Ekstrak Temulawak dilakukan pada jam ke 16-20. Uji kekerasan mikro menggunakan indenter Knoop sebelum dan sesudah perlakuan. Hasil : Sampai dengan jam ke 4, pH model biofilm yang terpapar Ekstrak Temulawak 25% tidak mengalami penurunan pH. Tidak terlihat efek Ekstrak Temulawak terhadap kekerasan permukaan email. Kesimpulan : Ekstrak Temulawak 25% mampu menghambat penurunan pH biofilm, tetapi tidak berpengaruh terhadap demineralisasi email. Xanthorrhizol isolated from Java turmeric is able to maintain the pH of biofilm model in vitro for 4 hours. It was known that MBC of Java turmeric extract was 25%. Purpose: To analyse the effect of 25% Java turmeric extract on email demineralization exposed to S. mutans biofilm. Methods: Biofilm model was obtained by culturizing S. mutans which was cultured on TYS Broth during 24 hours on 6 well- plates which was layered by pellicle. Java turmeric Extract was added to biofilm model at various duration between 1-48 hours. pH measurement using pH universal indicator. Biofilm model was also cultured at tooth sample surface. Java turmeric extract was added at 16-20 hours. Micro hardness test was conducted using Knoop indenter before and after the intervention. Result : After 4 hours, the pH of biofilm model which was exposed to Java turmeric 25% was not decreasing. No difference was found on the enamel micro hardness between experiment and control groups. Conclusion : Java turmeric 25% is able to prevent reduction of biofilm pH, but does not have effect on enamel demineralization. |
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| No. Panggil : | T31042 |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Entri tambahan-Nama badan : | |
| Subjek : | |
| Penerbitan : | Jakarta: Fakultas Kedokteran Gigi Universitas Indonesia, 2012 |
| Program Studi : |
| Bahasa : | ind |
| Sumber Pengatalogan : | LibUI ind rda |
| Tipe Konten : | text |
| Tipe Media : | unmediated ; computer |
| Tipe Carrier : | volume ; online resource |
| Deskripsi Fisik : | xvi, 52 pages ; illustration; 28 cm + appendix |
| Naskah Ringkas : | |
| Lembaga Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI, Lantai 3 |
| No. Panggil | No. Barkod | Ketersediaan |
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| T31042 | 15-18-051348743 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20308067 |
