The development of biochemical oxygen demand sensor using local yeast : candida fukuyamaensis UICC Y-247
Ivandini Tribidasari Anggraningrum, Fenny Triana Zulfia, Endang Saepudin ([Direktorat Riset dan Pengabdian Masyarakat UI;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, Universitas Indonesia], 2011)
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ABSTRACT In order to shorten the measurement time of biochemical oxygen demand (BOD), a BOD sensor based on yeast metabolism was developed. Local yeast, Indonesian Origin, Candida fukuyamaensis UICC Y-247, was used as a transducer. The yeast was immobilized as a thin film in agarose matrix with the auxiliary of Nafion® acting as the membrane for ion exchange process. The film was then attached to gold-modified glassy carbons and used as transducer on the working electrodes. The measurements were conducted by observing the depletion of glucose concentration using multipulse amperometric method and then converted to BOD values. Optimum condition was observed in a waiting measurement time of 30 min at an applied potential of 450 mV (vs.Ag/AgCl). Linearity was shown in glucose concentration range of 0.1?0.5 mM, which was equivalent to BOD concentration range of 10?50 mg/L. A detection limit of 1.13 mg/L BOD could be achieved. Good repeatability was shown by a relative standard deviation (RSD) of2.7% (n = 15). However, decreasing current response of ~50% was found after 3 days. Comparing to the conventional BOD measurement, this BOD sensor can be used as an alternative method for BOD measurements. |
| No. Panggil : | J-Pdf |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Subjek : | |
| Penerbitan : | [Place of publication not identified]: [Direktorat Riset dan Pengabdian Masyarakat UI;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia;Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, Universitas Indonesia], 2011 |
| Sumber Pengatalogan : | LibUI eng rda |
| ISSN : | 23391995 |
| Majalah/Jurnal : | Makara : Sains |
| Volume : | Vol. 15, No. 1, April 2011: 33-37 |
| Tipe Konten : | text |
| Tipe Media : | unmediated |
| Tipe Carrier : | volume |
| Akses Elektronik : | http://journal.ui.ac.id/index.php/science/article/view/874/833 |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Direktorat Riset dan Pengabdian Masyarakat UI |
| No. Panggil | No. Barkod | Ketersediaan |
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| J-Pdf | 03-19-047825304 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20326318 |
