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Purification and carbohydrate analysis of recombinant human erythropoietin expressed in yeast system pichia pastoris

Andri Wardiana, Adi Santoso ([Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011)

 Abstrak

ABSTRACT
For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni2+(His-Trap affinity chromatography) and continued with gel filtration chromatogram phy column to get purer protein. The carbohydrate group which is o ligosaccharide from the resulting pure protein then can be recognized by using N- and O-glycosidase. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with 4 N HCl in 100oC temperature for 6 hours and the result was applied on High Performance Liquid Chromatography (HPLC) column to learn the composition of its monosaccharide.

 Metadata

No. Panggil : J-Pdf
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Subjek :
Penerbitan : [Place of publication not identified]: [Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011
Sumber Pengatalogan : LibUI eng rda
ISSN : 23391995
Majalah/Jurnal : Makara : Sains
Volume : Vol. 15, No. 1, April 2011: 75-78
Tipe Konten : text
Tipe Media : unmediated
Tipe Carrier : volume
Akses Elektronik : http://journal.ui.ac.id/index.php/science/article/view/888/847
Institusi Pemilik : Universitas Indonesia
Lokasi : Direktorat Riset dan Pengabdian Masyarakat UI
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No. Panggil No. Barkod Ketersediaan
J-Pdf 03-19-846032585 TERSEDIA
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