Purification and carbohydrate analysis of recombinant human erythropoietin expressed in yeast system pichia pastoris
Andri Wardiana, Adi Santoso ([Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011)
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ABSTRACT For clinical purposes, pure protein and identification of carbohydrate structure from recombinant erythropoietin are needed. Purification was done by Immobilized Metal Affinity Chromatography (IMAC) column charged with Ni2+(His-Trap affinity chromatography) and continued with gel filtration chromatogram phy column to get purer protein. The carbohydrate group which is o ligosaccharide from the resulting pure protein then can be recognized by using N- and O-glycosidase. Pure oligosaccharide was hydrolyzed to produce various monosaccharide through incubation with 4 N HCl in 100oC temperature for 6 hours and the result was applied on High Performance Liquid Chromatography (HPLC) column to learn the composition of its monosaccharide. |
| No. Panggil : | J-Pdf |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Subjek : | |
| Penerbitan : | [Place of publication not identified]: [Direktorat Riset dan Pengabdian Masyarakat UI;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi;Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia. Pusat Penelitian Bioteknologi], 2011 |
| Sumber Pengatalogan : | LibUI eng rda |
| ISSN : | 23391995 |
| Majalah/Jurnal : | Makara : Sains |
| Volume : | Vol. 15, No. 1, April 2011: 75-78 |
| Tipe Konten : | text |
| Tipe Media : | unmediated |
| Tipe Carrier : | volume |
| Akses Elektronik : | http://journal.ui.ac.id/index.php/science/article/view/888/847 |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Direktorat Riset dan Pengabdian Masyarakat UI |
| No. Panggil | No. Barkod | Ketersediaan |
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| J-Pdf | 03-19-846032585 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20326484 |
