Photoelectrochemical detection of dengue - related oligonucleotide sequence using anthocyanin as a intercalating agent and electrochromic material
([Direktorat Riset dan Pengabdian Masyarakat Universitas Indonesia, Fakultas Kedokteran Universitas Indonesia], 2010)
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| World Health Organization (WHO) presupposes a confirmation of dengue virus infection diagnosis with two criteria,i.e. clinical and laboratory criteria. One of the basic methods used by most laboratories to diagnose dengue virus is todetect oligonucleotide sequence using a DNA amplification technique. In this research, the measurement of denguerelatedoligonucleotide was conducted by photoelectrochemical method. The presence of oligonucleotide sequence intarget DNA can be detected by DNA probe that is immobilized on TiO2 electrode. The DNA hybrid is then bound toelectrochromic substance like anthocyanin that generates current when it is subjected to light. The photocurrent isdirectly proportional to the number of target DNA. The aim of this research is to obtain photoelectrochemical systemthat has sensitivity and high responsiveness toward the change in oligonucleotide concentration, especially theapplicability of anthocyanin as a electrochromic substance and intercalating agent. Linearity (R2) generated from thechange of current in response to concentration changes of target DNA (in the concentration range of 0.75?3.00 nM) is0.9611. Thus, this method has the potential to be developed to detect the presence of dengue virus in biological sample. |
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| Penerbitan : | [Place of publication not identified]: [Direktorat Riset dan Pengabdian Masyarakat Universitas Indonesia, Fakultas Kedokteran Universitas Indonesia], 2010 |
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| Majalah/Jurnal : | Makara : Kesehatan |
| Volume : | Vol. 14, No. 2, Desember 2010: 70-74 |
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| Akses Elektronik : | http://journal.ui.ac.id/index.php/health/article/view/686/653 |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Direktorat Riset dan Pengabdian Masyarakat UI |
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