Long terminal repeats region as a potential target for HIV molecular detection / Aroem Naroeni, Hatiyowidi Yuliawuri, Fera Ibrahim, Budiman Bela
Aroem Naroeni, examiner
([Direktorat Riset dan Pengabdian Masyarakat UI;Universitas Indonesia. Institute of Human Virology and Cancer Biology, Institute of Human Virology and Cancer Biology University of Indonesia], 2010)
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| Indonesian National Committee for human immunodeficiency virus (HIV) and acquired immune deficiency syndrome(AIDS) has reported the significant increase of HIV infected individual in Indonesia. A sensitive accurate diagnosticsare urgently needed to prevent the dissemination of HIV and also to provide a suitable therapy. For this reason, we havedeveloped HIV diagnostic method based on PCR to elucidate this problem. For this research, samples were collectedfrom hospitals and Indonesian Red Cross that consist of samples possesing HIV serological test positive andindeterminate. Ribonucleic Acid (RNA) were isolated from blood plasma. These RNA then were amplified afterReverse transcriptase reaction by using primers which have been designed using env (C2V3C3), Long TerminalRepeats (LTR) (U3) and Capsid gag (p24) as target regions. The obtained results shown 26/34 (76.5%) positive in LTR,10/33 (36.4%) positive in Env and 11/33 (33.3%) positive in p24. These results showed that LTR primers detect HIVmore than other primers. It suggests that LTR could be used as detection target as complement of env and p24 Theseresults need to be explored further by using sequencing method. |
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| Penerbitan : | [Place of publication not identified]: [Direktorat Riset dan Pengabdian Masyarakat UI;Universitas Indonesia. Institute of Human Virology and Cancer Biology, Institute of Human Virology and Cancer Biology University of Indonesia], 2010 |
| Sumber Pengatalogan : | LibUI eng rda |
| ISSN : | 23563656 |
| Majalah/Jurnal : | Makara Journal of Health Research |
| Volume : | Vol. 14, No. 2, Desember 2010: Hal. : 86-88 |
| Tipe Konten : | text |
| Tipe Media : | computer |
| Tipe Carrier : | online resource |
| Akses Elektronik : | http://journal.ui.ac.id/index.php/health/article/view/689/656 |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Direktorat Riset dan Pengabdian Masyarakat UI |
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