Incubation of denaturated samples increased reproducibility and quality of proteomic profile of SELDI-TOF MS
by Muhareva Raekiansyah, T. Mirawati Sudiro (Institute of Human Virology and Cancer Biology, 2009)
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Protein profiling with high-throughput proteomic technology, SELDI-TOF, is a new potential tool for diagnosis of human diseases. This advanced technique has increasingly been used for the detection of disease biomarker. However, analytical reproducibility is a significant challenge in SELDI-TOF profiling in order to have confidence in the results. Here, we showed a simple step to improve its analytical performance. IMAC 30-Cu Protein Chip was used to incubate denaturated samples to increase the number of peak detection and decrease peak intensity coefficient of variation. Incubation of denaturated samples overnight at 4oC increased significantly reproducibility and quality of proteomic profile of SELDI-TOF MS for IMAC30-Cu ProteinChip. This strategy could be applied to address reproducibility issue in protemictechnology in protein profiling. |
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Penerbitan : | [Place of publication not identified]: Institute of Human Virology and Cancer Biology, 2009 |
Sumber Pengatalogan : | LibUI eng rda |
ISSN : | 23563656 |
Majalah/Jurnal : | Makara Journal of Health Research |
Volume : | Vol. 13, No. 1, Juni 2009: Hal. : 48-52 |
Tipe Konten : | text |
Tipe Media : | computer |
Tipe Carrier : | online resource |
Akses Elektronik : | http://journal.ui.ac.id/index.php/health/article/view/351/347 |
Institusi Pemilik : | Universitas Indonesia |
Lokasi : | Direktorat Riset dan Pengabdian Masyarakat UI |
No. Panggil | No. Barkod | Ketersediaan |
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03-19-065953506 | TERSEDIA |
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