Objektif. Preeklampsia adalah penyakit pada kehamilan ditandai dengan hipertensi dan proteinuria. Di Indonesia, preelampsia/eklampsia merupakan penyebab utama mortalitas dan morbiditas ibu dan anak. Stress oksidatif pada plasenta dan sistem sirkulasi menyebabkan disfungsi dan kerusakan sel endotel. Stres oksidatif di plasenta menyebabkan gangguan pertumbuhan janin. HSP70 adalah molekul protein yang sangat penting untuk penyembuhan sel dan menjaga homeostasis. Tujuan penelitian untuk membandingkan kadar MDA dan HSP70 yang diproduksi di plasenta pada kehamilan dengan preeklampsia berat, ringan dan kehamilan normal. Plasenta didonorkan secara sukarela dari ibu2 yang melahirkan dengan preeklampsia ringan (N=10), preeklampsia berat (N=10) dan kehamilan normal (N=10). Plasenta dikultur dengan RPMI dan FBS 20%, pada hari ke 3, supernatant diambil. Diperiksa kadar Malondealdehida (MDA), petanda untuk stres oksidatif dan kadar HSP70. Kadar MDA diukur dengan spektrofotometer pada panjang gelombang 530nm. Kadar HSP 70 diukur dengan metoda enzyme-linked immunosorbent assay. Kadar rata2 MDA pada preeklampsia berat (7,13+5,36 nmol/ml), preeklampsia ringan (4,82+2,47 nmol/ml) dan hamil normal (4,87+2,4 nmol/ml). Kadar MDA pada preeklampsia berat paling tinggi, tetapi perbedaan tersebut tidak berbeda bermakna. Kadar rata2 HSP70 pada preeklampsia ringan tertinggi (10,15+12,39 nmo/ml) dibandingkan dengan kadarnya pada preeklampsia berat (3,78 +3,07 nmol/ml) dan kehamilan normal (3,76+4,65nmol/ml), namun perbedaan ini tidak berbeda bermakna. Walaupun demikian, kadar HSP sangat tinggi pada preeklampsia ringan menunjukkan respons homeostasis relatif tinggi. Hal ini tidak ditunjukkan pada preeklampsia berat. Kadar rata2 MDA dan HSP70 pada preeklampsia berat, ringan maupun hamil normal tidak berbeda bermakna. Kadar HSP yang sangat tinggi pada preeklampsia ringan menunjukkan respons homeostasis masih tinggi.
Objective: Preeclampsia is a disease in pregnancy and characterized by hypertension and proteinuria. Preeclampsia and eclampsia are the most causes of maternal and fetal mortality and morbidity in Indonesia. Placental and systemic oxidative stress caused endothelial cell dysfunction and injury. Placental oxidative stress also linked to fetal growth restriction. HSP70 is essential for cellular recovery, survival and maintenance of homeostasis. The purpose of this study was to compare the MDA, a marker for oxidative stress and HSP70 production in placental of severe preeclampsia, mild preeclampsia and normotensive pregnant women. Placenta were collected after delivery from normotensive pregnancies (N=10), severe preeclampsia (N=10) and mild preeclampsia (N=10). Placenta was cultured in RPMI and 20% FBS, and supernatant were collected in day 3. MDA was measured using spectrophotometer and absorbance read in 530nm. HSP70 was measured using enzyme-linked immunosorbent assay. The mean MDA concentration did not differ significantly between patients with severe preeclampsia (7.13+5.36 nmol/ml) and mild preeclampsia (4.82+2.47 nmol/ml) when compared with normotensive pregnancies (4.57+2.4 nmol/ml). The mean HSP70 concentration in mild preeclampsia is highest (10.15+12.39 nmo/ml) when compared with severe preeclampsia (3.78 +3.07 nmol/ml) and normotensive pregnant women (3.76+4.65nmol/ml), but the difference was not significant. Although the difference was not significant, is indicates homeostasis response in mild preclampsia women is relative good. This response was abated in severe preeclampstic women. Although MDA and HSP70 concentration did not differ significantly between groups, however the high HSP70 concentration is indicates homeostasis response relatively good in mild preeclamptic women. |
