Deskripsi Lengkap

Tujuan: Menyelidiki perbedaan respons metabolik terhadap asupan diet mengandung orotat dan diet mengandung adenin pada profi l lipid dalam serum dan jaringan hati. Metode: Tikus-tikus percobaan diberi diet yang mengandung orotat 1.0% (kelompok orotat) dan adenin 0.25% (kelompok adenin) atau diet yang tak disuplementasi (kelompok kontrol) selama 10 hari. Kadar lipid dalam serum diukur menggunakan enzyme assay kits. Lipid jaringan hati diekstraksi dan konsentrasinya ditentukan. Hasil: Kadar serum lipid kelompok adenin cendrung meningkat, sebaliknya kelompok orotat cendrung menurun dibandingkan dengan kelompok kontrol. Kadar serum trigliserida (mg/dL) pada kelompok kontrol, orotat, dan adenin masing-masing (78,1±14,9), (69,0±23,6), dan (136,1±21,6); Kadar fosfolipid (PL): (109,2±11,5), (93,3±10,5), dan (131,3±11,0); Total kolesterol: (53,7±4,6), (42,9±6,5), dan (68,1±5,8); dan high-density lipoprotein (HDL)-kolesterol: (35,4±2,7), (33,0±3,0), dan (44,7±2,7). Kandungan TG hati pada kelompok orotat meningkat tajam mendekati 10-kali lipat dibandingkan dengan kelompok kontrol (P<0,05), sebaliknya kandungan TG kedua kelompok lainnya hampir sama. Kandungan lipid jaringan hati (mg/g jaringan) pada ketiga kelompok dimaksud masing-masing untuk TG adalah: (11,4±1,3), (123,5±15,2), dan (11,9±1,2); PL: (27,1±0,8), (25,4±1,3), dan (30,7±0,6); dan total kolesterol: (2,73±0,09), (2,34±0,12), dan (2,91±0,08). Kandungan PL dan kolesterol hati pada kelompok adenin masing-masing meningkat sebesar 21% dan 25% dibandingkan dengan kelompok orotat, tetapi kandungan kedua jenis lipid tersebut pada kelompok terakhir ini menurun masing-masing sebesar 7% dan 15% dibandingkan dengan kelompok kontrol. Kesimpulan: Dietary adenin menginduksi sekresi lipid ke dalam aliran darah dan transport balik kolesterol menuju sel-sel hati. Sebaliknya dietary orotat cendrung mempertahankan lipid-lipid yang telah disintesis di sel-sel hati, terutama TG, dan mengurangi sekresi.

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Abstract
Aim: Objectives To evalate the differences in metabolic responses between dietary orotic acid and adenine in lipid profi les of serum and liver tissues. Methods: Rats were paired-fed 1.0 % orotic acid (orotic acid group) and 0.25 % adenine (adenine group) diets or a non-supplemented diet (control group) for 10 days. Serum lipid concentrations were measured using enzyme assay kits. Lipids of liver tissues were extracted and the lipid contents were determined. Results: Serum lipid concentrations (in mg/dL) of adenine group tended to increase whereas those levels decreased in orotic acid group compared to control group. The serum triglyceride (TG) concentrations of control, orotic acid, and adenine groups were (78.1±14.9), (69.0±23.6), and (136.1±21.6); phospholipids (PL): (109.2±11.5), (93.3±10.5), and (131.3±11.0); total cholesterol: (53.7±4.6), (42.9±6.5), and (68.1±5.8); and high-density lipoprotein (HDL)-cholesterol: (35.4±2.7), (33.0±3.0), and (44.7±2.7), respectively. Furthermore, liver TG content of orotic acid group markedly increased. The increase was approximately by 10-fold in comparison to other groups (P<0.05). The lipid contents of liver tissues (in mg/g tissue) in ordinarily of those three groups for TG were (11.4±1.3), (123.5±15.2), and (11.9±1.2); PL: (27.1±0.8), (25.4±1.3), and (30.7±0.6); and the total cholesterol: (2.73±0.09), (2.34±0.12), and (2.91±0.08), respectively. The liver PL and cholesterol content of adenine group increased by 21% and 25% than that of orotic acid group, but both lipid levels of the latter group increased by 7% and 15%, respectively, than that of the control group. Conclusion: Dietary adenine enhances the serum TG, PL, cholesterol, and HDL-cholesterol and the liver PL and cholesterol but without alters the liver TG levels. Dietary orotic acid, however, attenuates these serum lipid levels but retains those lipids synthesized in liver cells, mainly TG.