Telah dilakukan penelitian untuk mengetahui daya antibakteri ekstrak n-heksana dan ekstrak etil asetat kulit batang manggis hutan (Garcinia rigida Miq.) terhadap kuman Salmonella typhosa ATCC 14028,Staphylococcus aureus ATCC 29213 dan Bacillus subtilis ATCC 6633. Penelitian dilakukan melalui penentuan zona hambatan pertumbuhan dengan metode difusi silinder dan kadar hambat minimal (KHM) denganmetode dilusi penapisan lempeng. Hasil Penelitian menunjukkan bahwa ekstrak n-heksana kulit batang Garcinia rigida Miq. Tidak memberikan zona hambatan terhadap pertumbuhan kuman Salmonella typhosa ATCC 14028,Staphylococcus aureus ATCC 29213 dan Bacillus subtilis ATCC 6633, tetapi memberikan nilai kadar hambat minimal pada konsentrasi 500 mg/ml untuk Salmonella typhosa ATCC 14028, 250 mg/ml untukStaphylococcus aureus ATCC 29213 dan 125 mg/ml untuk Bacillus subtilisATCC 6633, sedangkan ekstrak etil asetat kulit batang Garcinia rigida Miq. memberikan zona hambatan terhadap pertumbuhan pada konsentrasi 500, 250 dan 125 mg/ml berturut-turut untuk Salmonella typhosaATCC 14028 adalah 11,15; 9,05; 7,55 mm sedangkan untuk Staphylococcus aureus ATCC 29213 adalah 14,25; 11,10; 8,95 mm dan untuk Bacillus subtilis ATCC 6633 adalah 20,97; 15,00; 10,07 mm. Kadar hambat minimal untuk kadar ekstrak etil asetat berturut-turut untuk kumanSalmonella typhosa ATCC 14028, Staphylococcus aureus ATCC 29213 danBacillus subtilis ATCC 6633 adalah 250, 62,5 dan 31,25 mg/ml. Disimpulkan bahwa ekstrak etil asetat kulit batang manggis hutan (Garcinia rigida Miq.) memiliki daya antibakteri lebih baik dibandingkan dengan ekstrak n-heksana kulit batang manggis hutan (Garcinia rigida Miq.)
Abstract
A research on the antibacterial activity of n-hexane extract and the ethyl acetate Garcinia rigida Miq. Bark against Salmonella typhosaATCC 14028, Staphylococcus aureus ATCC 29213 dan Bacillus subtilisATCC 6633 has been carried out. The research was included the determination of the growth inhibition zona with the cylinder diffusion method and the minimum inhibitory concentration with the petri dish dilution method. The result of this study showed that the n-hexane extract of Garcinia rigida Miq.bark did not give the growth inhibition zona to Salmonella typhosa ATCC 14028, Staphylococcus aureus ATCC 29213 and Bacillus subtilis ATCC 6633, but gave the minimum inhibitory concentration at 500 mg/ml for Salmonella typhosaATCC 14028, 250 mg/ml for Staphylococcus aureus ATCC 29213 and 125 mg/ml for Bacillus subtilis ATCC 6633. Whereas the ethyl acetateextract of Garcinia rigida Miq. bark gave the growth inhibition zona of concentration 500, 250 and 125 mg/ml with average diameter toSalmonella typhosa ATCC 14028 were about 11.15, 9.05, 7.55 mm, toStaphylococcus aureus ATCC 29213 were about 14.25, 11.10, 8.95 mm and to Bacillus subtilis ATCC 6633 were about 20.97, 15.00, 10.07 mm. The minimum inhibitory concentration to ethyl acetate extract toSalmonella typhosa ATCC 14028, Staphylococcus aureus ATCC 29213and Bacillus subtilis ATCC 6633 were about 250, 62,5 and 31,25 mg/ml respectively. As a conclusion, the ethyl acetate extract ofGarcinia rigida Miq. bark hadmore better antibacterial activity than the n-hexane extract of Garcinia rigida Miq. Bark |
