ABSTRAKTujuan penelitian ini adalah untuk mengetahui suplernentasi vitamin B12 pada penderita HIV terhadap jumlah CD4, sehingga diharapkan dapat mencegah progresivitas penyakit HIV. Penelitiam ini merupakan uji klinis tanpa pembanding, terhadap 15 orang pasien HIV di poliklinik UPT HIV RSUPNCM Jakarta mulai satu Februari sampai dengan 20 April 2010. Subyek mendapat suplementasi vitamin B12 (metilkobalamin) 1000 pg/had, peroral, sclama enam minggu. Data dikumpulkan meliputi data demografi (usia dan jenis kelamin), adanya hepatitis gastroenteritis dan infeksi akut selama penelitian, status gizi (indeks massa tubuh), analisis asupan zat gizi dengan metode had record 3 x24 jam dan FFQ semikuantitatit2 lcadar vitamin B12 serum dan jumlah CD4. Analisis data menggunakan uji t berpasangan atau Wiicoxon dengan batas kemaknaan p < 0,05. Sebanyak 15 subyek mengikuti penelitian sampaj sclesai. Setelah enam minggu perlakuan, didapatkan adanya peningkatan yang bennakna terhadap kadar vitamin Bn serum awal 270,71 i 71,04 pmol/L, pada akhir perlakuan 419,11 =4= 122,95 pmol/L meningkat signiiikan (p > 0,001). Terdapat 11 dari 15 subyek mengalami peningicatan jumlah CD4 pada akhir penelitian. Median jumlah CD4 subyek pada awal penelitian 143 (23 - 372) sei/pL dibandingkan dengan median pada akhir pcrlakuan 166 (18 - 428) /pL, didapatkan perubahan signifikan (p = 0,03l). Uji korelasi Spearman, tidak menunjukan korelasi bermakna antara perbedaan jumlah CD4 dengan perbedaan kadar vitamin B12 serum (r= -0,375, p= 0,l68). Dcngan demikian dapat disimpulkan bahwa, walaupun tidak terdapat korelasi pada perbedaan jumlah CD4 dan kadar vitamin B12, namun suplementasi vitamin B12 menggunakan metilkobalamin 1000 pg/hari, peroral, selama enam minggu pada penderita HIV dapat meningkatkan secara bcrmakna kadar vitamin Bn serum dan terdapat perubahan bermakna jumlah CD4.
ABSTRACTThe aim of this study is to find the effect of Vitamin B12 supplementation in HIV patients on the counts of CD4 so it would prevent the HIV progressiveness in RSUPNCM Jakarta. It is an one-armed clinical trial in 15 HIV patients in UPT HIV RSUPNCM Jakarta. The subjects received vitamin B12 (methylcobalamin) supplementation 1000 ug/day, per oral, for six weeks. The data was collected included demographic data (age and sex), the presence of hepatitis co-infection and gastroente1itis,and acute infection during nutritional status (body mass index), nutrition intake analysis with 3 x24 hours food record method and semi-quantitative FFQ, the level of serum vitamin B12 and CD4 counts. The study used paired t-test or Wilcoxon with significant value p < 0,05. There were I5 subjects who completely participated. After six weeks of intervention, there as a significant increment of early serum Vitamin Bl; level which was 270,71 1 71,04 pmol/L, and at the end ofthe intervention was 419,11 :h 122,95 pmol/L; increased significantly (p > 0,00l). There were ll of 15 subjects who had an increment at the end of the study. Early CD4 counts at the beginning of the study was 143 (23 - 372) cells/pL then changed significantly at the end of the study which was 166 (18 - 428) cells/pl., p = 0,03l. Though there was no significant correlation in CD4 counts difference to serum vitamin B12 level (r= -0,375, p= 0,l68)- It can be concluded that after six week intervention with vitamin B12 supplementation in methycobalamin form 1000 ug/day, per oral, in HIV patients would significantly increase serum vitamin B12 level and would significantly change CD4 counts, even-though there was no correlation on CD4 difference and vitamin Bl; level difference. |
