ABSTRAK
Pendahuluan: Sambiloto atau Andrographis panniculata merupakan sebuahtanaman herbal yang memiliki khasiat sebagai antimalaria dengan carameningkatkan kerja antioksidan dalam tubuh. Hati merupakan salah satu tempatterjadinya fase perkembangan Plasmodium pada penyakit malaria. Penelitian inibertujuan untuk menganalisis aktivitas antimalaria dari Ekstrak Etanol Sambiloto(EES) pada mencit yang diiinfeksi Plasmodium berghei secara in vivo melalupengukuran kadar MDA dan aktivitas spesifik katalase jaringan hati.Metode: Desain penelitian yang digunakan adalah eksperimental in vivomenggunakan hewan coba mencit Balb/c. Metode penelitian dilakukan denganmengelompokkan mencit ke dalam empat kelompok yaitu kelompok kontrol yangtidak diberi perlakuan, kelompok I yang diinduksi Plasmodium berghei tetapitidak diterapi, kelompok II yang diinduksi Plasmodium berghei dan diberi EES 2mg/kgBB serta kelompok III yang diinduksi Plasmodium berghei dan diberiklorokuin 10 mg/kgBB selama 3 hari. Analisis kadar MDA dilakukan denganmetode Wills dan aktivitas spesifik katalase dengan metode Mates et al.Hasil: Hasil penelitian menunjukkan terjadi penurunan kadar MDA yang tidaksignifikan pada mencit yang diinfeksi dengan Plasmodium berghei dan diberiekstrak etanol sambiloto (EES) 2 mg/kgBB dibandingkan dengan kontrol negatif(66.49 ± 22,92 vs 69.40 ± 11,69 nmol/g jaringan hati). Namun pada kelompokyang diberi perlakuan klorokuin juga terlihat penurunan kadar MDA yang tidaksignifikan dibandingkan dengan kontrol negatif (67.49 ± 7,04 vs 69.40 ± 11,69nmol/g jaringan hati). Sedangkan aktivitas spesifik katalase kelompok yang diberiEES menunjukkan peningkatan yang tidak berbeda bermakna dibandingkandengan kelompok kontrol (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg jaringan hati).Begitupula dengan klorokuin yang menunjukkan peningkatan aktivitas spesifikkatalase yang tidak berbeda bermakna dibandingkan dengan kelompok kontrol(2,97 ± 1,53 vs 3,73 ± 1.56).Kesimpulan: Pada kelompok dengan pemberian EES 2 mg/kgBB terjadipenurunan kadar MDA serta peningkatan aktivitas spesifik katalase jaringan hatimencit dibandingkan dengan kelompok negatif, tetapi secara statistik tidakbermakna demikian pula dengan kelompok yang diberi klorokuin.
ABSTRACT
Introduction: Andrographis panniculata or Sambiloto is a herbal plant that hasantimalarial efficacy by increasing antioxidant in body. Liver is one of the placesfor Plasmodium to develop themselves in malaria. This research aims to analyzethe activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice whichinfected by Plasmodium berghei in vivo through the measurement of MDA leveland the specific activity of catalase in liver tissue.Method: We used experimental in vivo as the reserach design, using balb/c. Theresearch design is done by grouping the mices into four groups which of theuntreated group, group I-induced by Plasmodium berghei but not treated, groupII-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,group III-induced Plasmodium berghei and treated with chloroquine with 10mg/kg Body weight in three days. The MDA level analyze is done by the Willsmethod and the specific activity of catalase with Mates et al method.Result: The research result showed the decrease of MDA level which notsignificant in mice that is infected by Plasmodium berghei and treated by SEE 2mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/gliver tissue). However, group that is infected by Plasmodium berghei and treatedby chloroquine also showed the decrease of MDA level which not significantcompared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).Instead, group which treated by SEE showed the increase in specific activity ofcatalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).Similarly with chloroquine group which showed an increase in specific activity ofcatalase were not significantly different compared with the control group (2.97 ±1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decreaseof MDA level and also the increase of catalase specific activity in mice liver tissuecompared negative control, but statistically not significant as well as the groupgiven chloroquine;Introduction: Andrographis panniculata or Sambiloto is a herbal plant that hasantimalarial efficacy by increasing antioxidant in body. Liver is one of the placesfor Plasmodium to develop themselves in malaria. This research aims to analyzethe activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice whichinfected by Plasmodium berghei in vivo through the measurement of MDA leveland the specific activity of catalase in liver tissue.Method: We used experimental in vivo as the reserach design, using balb/c. Theresearch design is done by grouping the mices into four groups which of theuntreated group, group I-induced by Plasmodium berghei but not treated, groupII-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,group III-induced Plasmodium berghei and treated with chloroquine with 10mg/kg Body weight in three days. The MDA level analyze is done by the Willsmethod and the specific activity of catalase with Mates et al method.Result: The research result showed the decrease of MDA level which notsignificant in mice that is infected by Plasmodium berghei and treated by SEE 2mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/gliver tissue). However, group that is infected by Plasmodium berghei and treatedby chloroquine also showed the decrease of MDA level which not significantcompared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).Instead, group which treated by SEE showed the increase in specific activity ofcatalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).Similarly with chloroquine group which showed an increase in specific activity ofcatalase were not significantly different compared with the control group (2.97 ±1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decreaseof MDA level and also the increase of catalase specific activity in mice liver tissuecompared negative control, but statistically not significant as well as the groupgiven chloroquine;Introduction: Andrographis panniculata or Sambiloto is a herbal plant that hasantimalarial efficacy by increasing antioxidant in body. Liver is one of the placesfor Plasmodium to develop themselves in malaria. This research aims to analyzethe activity of antimalarial from Sambiloto Ethanol Extract (SEE) in mice whichinfected by Plasmodium berghei in vivo through the measurement of MDA leveland the specific activity of catalase in liver tissue.Method: We used experimental in vivo as the reserach design, using balb/c. Theresearch design is done by grouping the mices into four groups which of theuntreated group, group I-induced by Plasmodium berghei but not treated, groupII-induced Plasmodium berghei and treated with SEE 2 mg/kg Body weight,group III-induced Plasmodium berghei and treated with chloroquine with 10mg/kg Body weight in three days. The MDA level analyze is done by the Willsmethod and the specific activity of catalase with Mates et al method.Result: The research result showed the decrease of MDA level which notsignificant in mice that is infected by Plasmodium berghei and treated by SEE 2mg/ kg BW compared to negative control (66.49 ± 22,92 vs 69.40 ± 11,69 nmol/gliver tissue). However, group that is infected by Plasmodium berghei and treatedby chloroquine also showed the decrease of MDA level which not significantcompared the negative control (67.49 ± 7,04 vs 69.40 ± 11,69 nmol/g liver tissue).Instead, group which treated by SEE showed the increase in specific activity ofcatalase compared with control (2,73 ± 0,59 vs 3,73 ± 1.56 Unit/mg liver tissue).Similarly with chloroquine group which showed an increase in specific activity ofcatalase were not significantly different compared with the control group (2.97 ±1.53 vs 3.73 ± 1.56 Unit/mg liver tissue).Conclusion: Group that treated with SEE 2 mg/kg Body weight showed decreaseof MDA level and also the increase of catalase specific activity in mice liver tissuecompared negative control, but statistically not significant as well as the groupgiven chloroquine |
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