Protein Non Struktural-1 (NS-1) dari virus dengue terbukti menjadi penandauntuk diagnosis awal infeksi dengue. Bio-Rad NS1 Ag adalah salah satu alatdiagnostikdi Indonesia yang menggunakan NS1.Tujuan dari penelitian ini adalahuntuk mengukur sensitivitas dan spesifisitas kit pada virus dengue serotype-2(DENV-2), virus dengue serotype-4 (DENV-4) dan infeksi campuran Ujidiagnostik dilakukan selama36 bulan (Maret2010 ?Februari 2013). Sebanyak 102pasien dengan demam kurang dari 48 jam memenuhi kriteria inklusi dalam studiini. Reverse Transcripion-Polymerase Chain Reaction (RT-PCR) atau isolasivirus di cell line C6/36 atau kenaikan titer antibody dijadikan sebagai standarbaku penentu infeksi dengue. RT-PCR juga digunakan untuk menentukan serotipevirus. Analisis statistik dilakukan dengan menggunakan SPSS versi 17.0. Variabeldata binomial disajikan dalam interval kepercayaan 95%. Sensitivitas danspesifisitas kit diagnostik disajikan dalam tabel 2x2 dan area di bawah kurva(AUC) dari Receiver Operating Characteristics Curve. Dari 102 pasien secaraberurutan didapatkan DENV-1, DENV-2, DENV-3 dan DENV-4, adalah 17(16.7%), 21 (20.5%), 16 (15.7%) dan 4 (3.9%). Pada penelitian ini jugaditemukan infeksi campuran yaitu campuran (i)DENV-1 dan DENV-2, (ii)DENV-1 dan DENV-3, (iii)DENV-1 dan DENV-4, (iv)DENV-1, DENV-3, dan DENV-4,(v)DENV-2 dan DENV-4 dan (vi)serotipe yang tidak diketahui secara berurutanadalah 2 (2.0%), 3 (2.9%), 1 (1.0%), 1 (1.0%), 1 (1.0%), dan 2 (2.0%).Sensitivitas dan spesifisitas masing Bio-Rad NS1 Ag Strip untuk mendeteksiinfeksi DENV-2 adalah 76,2% dan 100% (95% CI, 76.8% to 99.3%). Sementaraitu, sensitivitas dan spesifisitas strip untuk mendeteksi infeksi DENV-4 adalah50% dan 100% (95% CI, 50% to 100%). Sensitivitas dan spesifisitas Bio-RadNS1 Ag Strip pada infeksi campuran adalah 100% (95% CI, 95% to 100%). Bio-Rad NS1 Ag memiliki sensitivitas tinggi dan spesifisitas untuk menentukaninfeksi akut DENV-2 dan infeksi campuran. Namun, Bio-Rad NS1 Ag memilikisensitivitas yang terbatas, namun spesifisitas tinggi untuk diagnosis infeksi akutDENV-4.;Non Structural-1 (NS1) protein of dengue virus is proven to be a marker for earlydiagnosis of dengue infection. Bio-Rad NS1 Ag Strip is one of the availablediagnostic kit in Indonesia that comprised of NS1. The aim of this study was tomeasure the sensitivity and specificity of the kit within DENV-2, DENV-4andmixed dengue virus serotypes. This study was done in 36 months (March 2010-February 2013). There were 102 dengue suspected patients with fever less than 48hours was fulfilling inclusion criteria. Reverse Transcription- Polymerase ChainReaction (RT-PCR) or virus isolation in C6/36 cell line or increase titer antibodyby ELISA was used as gold standard. RT-PCR was also used to determineserotype of the dengue virus. SPSS version 17.0 was the main statistical tool thatwe used. Data binominal variables was presented as incidence rates with 95%confidence intervals. Sensitivity and specificity of diagnostic kit was presented in2x2 tablesand area under the curve (AUC) of the Receiver OperatingCharacteristics Curve. From 102 patients, 68 (68.3%) patients were confirmedpositive dengue infection. Within confirmed dengue infection patients by RT-PCRwe found DENV-1, DENV-2, DENV-3,DENV-4 17 (16.7%), 21 (20.5%), 16(15.7%), 4 (3.9%), respectively. It was also found some mixed infection cases,which were (i)DENV-1 and DENV-2, (ii)DENV-1 and DENV-3, (iii)DENV-1and DENV-4, (iv)DENV-1, DENV-3 and DENV-4, (v)DENV-2 and DENV-4 and(vi)unknown were of 2 (2.0%), 3 (2.9%), 1 (1.0%), 1 (1.0%), 1 (1.0%) and 2(2.0%), respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip forDENV-2 sera collected were 76.2% and 100% (95% CI, 76.8% to 99.3%),respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip for DENV-4were 50% and 100% (95% CI, 50% to 100%), respectively. The sensitivity andspecificity of Bio-Rad NS1 Ag Strip for mixed infection were both 100% (95%CI, 95% to 100%). Bio-Rad NS1 Ag Strip has high sensitivity and specificity todetermine DENV-2 and mixed infection. On the other hand, Bio-Rad NS1 AgStrip has limited sensitivity, but high specificity for diagnosis of DENV-4 acuteinfection;Non Structural-1 (NS1) protein of dengue virus is proven to be a marker for earlydiagnosis of dengue infection. Bio-Rad NS1 Ag Strip is one of the availablediagnostic kit in Indonesia that comprised of NS1. The aim of this study was tomeasure the sensitivity and specificity of the kit within DENV-2, DENV-4andmixed dengue virus serotypes. This study was done in 36 months (March 2010-February 2013). There were 102 dengue suspected patients with fever less than 48hours was fulfilling inclusion criteria. Reverse Transcription- Polymerase ChainReaction (RT-PCR) or virus isolation in C6/36 cell line or increase titer antibodyby ELISA was used as gold standard. RT-PCR was also used to determineserotype of the dengue virus. SPSS version 17.0 was the main statistical tool thatwe used. Data binominal variables was presented as incidence rates with 95%confidence intervals. Sensitivity and specificity of diagnostic kit was presented in2x2 tablesand area under the curve (AUC) of the Receiver OperatingCharacteristics Curve. From 102 patients, 68 (68.3%) patients were confirmedpositive dengue infection. Within confirmed dengue infection patients by RT-PCRwe found DENV-1, DENV-2, DENV-3,DENV-4 17 (16.7%), 21 (20.5%), 16(15.7%), 4 (3.9%), respectively. It was also found some mixed infection cases,which were (i)DENV-1 and DENV-2, (ii)DENV-1 and DENV-3, (iii)DENV-1and DENV-4, (iv)DENV-1, DENV-3 and DENV-4, (v)DENV-2 and DENV-4 and(vi)unknown were of 2 (2.0%), 3 (2.9%), 1 (1.0%), 1 (1.0%), 1 (1.0%) and 2(2.0%), respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip forDENV-2 sera collected were 76.2% and 100% (95% CI, 76.8% to 99.3%),respectively. The sensitivity and specificity of Bio-Rad NS1 Ag Strip for DENV-4were 50% and 100% (95% CI, 50% to 100%), respectively. The sensitivity andspecificity of Bio-Rad NS1 Ag Strip for mixed infection were both 100% (95%CI, 95% to 100%). Bio-Rad NS1 Ag Strip has high sensitivity and specificity todetermine DENV-2 and mixed infection. On the other hand, Bio-Rad NS1 AgStrip has limited sensitivity, but high specificity for diagnosis of DENV-4 acuteinfection |