[ABSTRAK
Garcinia merupakan salah satu tumbuhan di Indonesia yang mempunyai potensisebagai antioksidan sehingga dapat dimanfaatkan untuk mengobati berbagaimacam penyakit. Beberapa senyawa aktif pada marga Garcinia yang memilikiaktivitas sebagai antioksidan diantaranya xanton, flavonoid dan alkaloid.Penelitian ini dilakukan untuk mengetahui aktivitas antioksidan dan golongansenyawa kimia daun Garcinia daedalanthera Pierre yang merupakan salah satuspesies dari marga Garcinia. Pengukuran aktivitas antioksidan dilakukanmenggunakan metode DPPH. Hasil uji aktivitas antioksidan menunjukkan bahwaekstrak n-heksan, etil asetat, dan metanol memiliki aktivitas sebagai antioksidan,dengan nilai IC50 berturut-turut yaitu 56, 780; 9,040; dan 12,838 μg/mL. Padaekstrak etil asetat dilakukan fraksinasi menggunakan kromatografi cair vakum(KCV) dan diperoleh delapan fraksi gabungan berdasarkan hasil KLT yaituA,B,C,D,E,F,G, dan H. Fraksi G merupakan fraksi paling aktif dengan nilai IC50sebesar 4,673 μg/mL. Identifikasi golongan senyawa kimia pada fraksi Gmenunjukkan adanya flavonoid, alkaloid dan saponin;
ABSTRACT
Garcinia is one of the plants in Indonesia that have potential as antioxidants which can be used totreat various diseases. Some of the active compounds in Garcinia genus which have antioxidantactivity are xanthones, flavonoids and alkaloids. The study was conducted to determine theantioxidant activity and screening chemical compounds from leaves of Garcinia daedalantheraPierre, which is one species of the genus Garcinia. Measurement of antioxidant activity carriedout using the DPPH method. The results showed that the extracts of n-hexane, ethyl acetate, andmethanol have antioxidant activity, with IC50 values were 56,780; 9,040 and 12,838 μg/mL,respectively. In the ethyl acetate extract fractionation performed using vacuum liquidchromatography and obtained eight fractions combined based on TLC results of the A, B, C, D,E, F, G, and H. The fraction G was the most active fraction with IC50 value of 4,673 μg/mL. Thescreening of chemical compounds in the fraction of G showed flavonoids, alkaloids andsaponins, Garcinia is one of the plants in Indonesia that have potential as antioxidants which can be used totreat various diseases. Some of the active compounds in Garcinia genus which have antioxidantactivity are xanthones, flavonoids and alkaloids. The study was conducted to determine theantioxidant activity and screening chemical compounds from leaves of Garcinia daedalantheraPierre, which is one species of the genus Garcinia. Measurement of antioxidant activity carriedout using the DPPH method. The results showed that the extracts of n-hexane, ethyl acetate, andmethanol have antioxidant activity, with IC50 values were 56,780; 9,040 and 12,838 μg/mL,respectively. In the ethyl acetate extract fractionation performed using vacuum liquidchromatography and obtained eight fractions combined based on TLC results of the A, B, C, D,E, F, G, and H. The fraction G was the most active fraction with IC50 value of 4,673 μg/mL. Thescreening of chemical compounds in the fraction of G showed flavonoids, alkaloids andsaponins] |
S45665-Uji aktivitas.pdf :: Unduh