[ABSTRAK
Latar belakang. Transfusi trombosit ditujukan untuk mencegah dan mengatasi perdarahanpada pasien trombositopenia. Trombosit dapat mengalami aktivasi walaupun tidak terjadiperdarahan sehingga dapat menimbulkan suatu keadaan yang disebut hiperagregasi sepertipada trombosit dari seseorang dengan hiperlipidemia. AABB menganjurkan untukmembuang semua produk darah yang berasal dari donor dengan plasma yang lipemia.Penelitian ini bertujuan untuk mengetahui pengaruh status lipid donor trombosit aferesisterhadap fungsi trombosit dan kadar malondialdehid selama penyimpanan.Metodologi. Penelitian ini menggunakan desain deskriptif analitik pada 31 sediaan trombositaferesis yang berasal dari donor trombosit aferesis yang memenuhi kriteria inklusi daneksklusi. Sediaan trombosit aferesis dibagi menjadi dua grup, yaitu grup hiperlipidemia dannormolipidemia. Dilakukan pengujian terhadap kandungan trombosit, fungsi agregasi dankadar MDA pada hari pertama, kedua dan keempat penyimpanan.Hasil. Terjadi peningkatan kandungan trombosit selama penyimpanan pada kedua grup, yangberhubungan dengan proses apoptosis. Pada hari keempat terjadi kenaikan kandungantrombosit yang lebih banyak pada grup hiperlipidemia. Pada hari kedua didapatkan perbedaanyang bermakna pada agregasi trombosit dengan agonis ADP 2 μM. Pada hari keempatdidapatkan perbedaan kadar MDA yang bermakna. Didapatkan korelasi yang positif danbermakna antara kolesterol total, LDL dan trigliserida terhadap kadar MDA. Tidakdidapatkan korelasi yang bermakna antara kolesterol total, trigliserida dan kadar MDAterhadap agregasi trombosit.Simpulan. Status lipid donor meningkatkan terjadinya apoptosis trombosit aferesis, lebihsensitif terhadap agonis ADP dan peningkatan kadar MDA. Perlunya mengingatkan donortrombosit aferesis untuk diet rendah lemak sebelum proses aferesis dilaksanakan. Perlunyapenelitian lebih lanjut untuk menentukan kadar lipid yang masih dapat ditoleransi.
ABSTRACT
Background. Platelet transfusions is intended to prevent and resolve bleeding in patientswith thrombocytopenia. Platelet activation may have occured although there were nobleeding that can lead to a condition called hyperaggregation as in someone withhyperlipidemia. AABB recommends to dispose of all products from donors with plasmalipemia. This study aimed to determine the effect of lipid status of the donor plateletapheresis to platelet function and levels of malondialdehyde with in storage.Methodology. This study used descriptive analytic design in 31 platelet apheresisconcentrates. Samples were divided into two groups, hyperlipidemia and normolipidemia.The assay for the content of platelets, aggregation functions and levels of MDA was tested onthe first day, second and fourth of platelet storage.Results. An increase in the content of platelets during storage in both groups, which areassociated with the process of apoptosis. On the fourth day there was higher of contentsplatelets in hyperlipidemic grup than normolipidemic grup. There were significant differencein platelet aggregation with ADP 2 μM at second day and levels of MDA at fourth day. Therewere positive and significant correlations between total cholesterol, LDL and triglyceride tothe levels of MDA. There were no significant correlation between total cholesterol,triglycerides and MDA levels to platelet aggregation.Conclusion. Improved of the lipid status of the donor platelet apheresis will increase plateletapoptosis, more sensitive to agonist ADP and increase MDA levels. The need to reminddonors platelet apheresis to a low fat dietary before apheresis process implemented. Need forfurther research to determine the lipid levels that can still be tolerated., Background. Platelet transfusions is intended to prevent and resolve bleeding in patientswith thrombocytopenia. Platelet activation may have occured although there were nobleeding that can lead to a condition called hyperaggregation as in someone withhyperlipidemia. AABB recommends to dispose of all products from donors with plasmalipemia. This study aimed to determine the effect of lipid status of the donor plateletapheresis to platelet function and levels of malondialdehyde with in storage.Methodology. This study used descriptive analytic design in 31 platelet apheresisconcentrates. Samples were divided into two groups, hyperlipidemia and normolipidemia.The assay for the content of platelets, aggregation functions and levels of MDA was tested onthe first day, second and fourth of platelet storage.Results. An increase in the content of platelets during storage in both groups, which areassociated with the process of apoptosis. On the fourth day there was higher of contentsplatelets in hyperlipidemic grup than normolipidemic grup. There were significant differencein platelet aggregation with ADP 2 μM at second day and levels of MDA at fourth day. Therewere positive and significant correlations between total cholesterol, LDL and triglyceride tothe levels of MDA. There were no significant correlation between total cholesterol,triglycerides and MDA levels to platelet aggregation.Conclusion. Improved of the lipid status of the donor platelet apheresis will increase plateletapoptosis, more sensitive to agonist ADP and increase MDA levels. The need to reminddonors platelet apheresis to a low fat dietary before apheresis process implemented. Need forfurther research to determine the lipid levels that can still be tolerated.] |
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