[Penyakit infeksi merupakan salah satu masalah utama di negara berkembang saat ini.Keadaan ini memicu peningkatan frekuensi pemakaian anti mikroba sehingga resikopanresisten semakin besar. Tujuan penelitian ini adalah untuk melihat gambaran MICmikroba panresisten terhadap anti mikroba yang paling sering digunakan di lingkunganRSUPNCM. Mikroba yang diuji merupakan mikroba panresisten yang terbanyak berdasarkanpeta kuman yang disusun oleh Loho dkk pada bulan juli - desember 2012.Penelitian potong lintang ini terdiri dari 90 mikroba panresisten yaitu Acinetobacterspp, Pseudomonas spp dan Klebsiella spp. Data karakteristik sampel pemeriksaan dicatat dandilakukan uji resistensi. Uji resistensi mengunakan metode difusi cakram dan metode MICstrip test kemudian dicari 'interpretative error' kedua metode.Pada ketiga mikroba yang panresisten dengan metode difusi cakram diketahui bahwamasih terdapat nilai intermediate dan sensitif dengan metode MIC strip test. PadaAcinetobacter spp nilai sensitif paling tinggi didapat pada anti mikroba CS sebesar 55 isolat(100%) dan diikuti dengan anti mikroba AK sebanyak 7 isolat (12,7%) dan oleh CAS 1 isolat(1.8%). Nilai intermediate tertinggi terdapa pada AK yaitu 6 isolat (10,9%) yang diikuti olehCAS 1 isolat (1.8%). Pada Pseudomonas spp yang diperoleh nilai sensitif terbesar pada antimikroba CS 17 isolat (89.5%) diikuti dengan AK yaitu sebesar 3 isolat (15.7%), Nilaiintermediate tertinggi terdapat pada AK yaitu 6 isolat (31,5%) yang diikuti oleh CAS 4 isolat(21.1%) dan CS 2 isolat (10.5%). Klebsiella spp dengan jumlah 16 isolat didapatkan nilaisensitif tertinggi pada CS 16 isolat (100%) diikuti DOR dengan 2 isolat (12.5%) dankemudian oleh AK dan CAS dengan masing masing 1 isolat (6.25%). Nilai intermediateterdapat pada DOR dan AK dengan masing masing 1 isolat (6.25%).Hasil nilai ''interpretative error'' menunjukan tidak ditemukan VME. Nilai MJE danMNE Acinetobacter spp yang melebihi kriteria FDA adalah AK yaitu 12.7% dan 10.9%.Pada Pseudomonas spp nilai MJE yang melebihi kriteria FDA adalah AK, CAS dan DORyaitu 15.7%, 10,5% dan 5,2%. Nilai MNE yang melebihi kriteria FDA adalah AK, CAS danCS yaitu 31,5%, 21,1% dan 10,5%. Pada Klebsiella spp nilai MJE pada DOR, AK dan CASyaitu 12.5%, 6.25% dan 6.25%. Nilai MNE pada AK dan DOR yaitu 6.25% dan 6.25%.Kami menyimpulkan bahwa untuk mikroba yang panresisten dengan metode difusicakram dapat digunakan MIC test.;Infectious diseases are one of the major problems in developing countries today. Thiscondition triggers an increase in frequency of anti-microbial used that leads increasepanresistance. The purpose of this study was to descriptive of the MIC microbes resistant toanti-microbial is most often used in RSUPNCM. Microbes tested is the most resistantmicrobes germs based map compiled by Loho et al in July - December 2012.This cross-sectional who study consisted of 90 microbes resistant ie. Acinetobacterspp, Pseudomonas spp and Klebsiella spp. The characteristics of the data sample are recordedand performed resistance test. Resistance test its done using the disc diffusion and MIC teststrip method and then analized 'interpretative error' for both methods.On the third microbial panresisten with disc diffusion method is known that there areintermediate values and sensitive with MIC method. In Acinetobacter spp sensitive highestvalue whose obtained on the anti- microbial CS by 55 isolates (100 %) followed by antimicrobialand AK were 7 isolates (12.7 %) and by CAS 1 isolates ( 1.8 % ). The highestintermediate value ie there exist at AK 6 isolates (10.9 %) , followed by CAS 1 isolates (1.8%). In Pseudomonas spp were most sensitive to the values whose obtained anti- microbial CS17 isolates (89.5 %) followed by the AK is equal to 3 isolates (15.7 %) , the highestintermediate value that is contained in AK 6 isolates ( 31.5 % ) , followed by CAS 4 isolates(21.1 %) and CS 2 isolates (10.5 %) . Klebsiella spp isolates whose obtained with the number16, the highest score on the CS 16 sensitive isolates (100 %) followed DOR with 2 isolates(12.5 %) and then by AK and CAS with each 1 isolates (6,25 %). Intermediate valuescontained in DOR and AK with each 1 isolates ( 6,25 %) .Results of 'interpretative error'' is not found in Very major error. Minor error andMajor error Acinetobacter spp that exceeds the FDA criteria are AK is 12.7% and 10.9%. InPseudomonas spp Major error value that exceeds the FDA criteria are AK, CAS and DORwith 5.7%, 10.5% and 5.2%. The Minor error value that exceeds the FDA criteria are AK,CAS and CS are 31.5%, 21.1% and 10.5%. In Klebsiella spp, Major error on DOR, AK andCAS with 12.5%, 6:25% and 6:25%. The Minor error value on AK and DOR is 6:25% and6:25%.We conclude that MIC test can be used for microbial panresisten with the discdiffusion., Infectious diseases are one of the major problems in developing countries today. Thiscondition triggers an increase in frequency of anti-microbial used that leads increasepanresistance. The purpose of this study was to descriptive of the MIC microbes resistant toanti-microbial is most often used in RSUPNCM. Microbes tested is the most resistantmicrobes germs based map compiled by Loho et al in July - December 2012.This cross-sectional who study consisted of 90 microbes resistant ie. Acinetobacterspp, Pseudomonas spp and Klebsiella spp. The characteristics of the data sample are recordedand performed resistance test. Resistance test its done using the disc diffusion and MIC teststrip method and then analized 'interpretative error' for both methods.On the third microbial panresisten with disc diffusion method is known that there areintermediate values and sensitive with MIC method. In Acinetobacter spp sensitive highestvalue whose obtained on the anti- microbial CS by 55 isolates (100 %) followed by antimicrobialand AK were 7 isolates (12.7 %) and by CAS 1 isolates ( 1.8 % ). The highestintermediate value ie there exist at AK 6 isolates (10.9 %) , followed by CAS 1 isolates (1.8%). In Pseudomonas spp were most sensitive to the values whose obtained anti- microbial CS17 isolates (89.5 %) followed by the AK is equal to 3 isolates (15.7 %) , the highestintermediate value that is contained in AK 6 isolates ( 31.5 % ) , followed by CAS 4 isolates(21.1 %) and CS 2 isolates (10.5 %) . Klebsiella spp isolates whose obtained with the number16, the highest score on the CS 16 sensitive isolates (100 %) followed DOR with 2 isolates(12.5 %) and then by AK and CAS with each 1 isolates (6,25 %). Intermediate valuescontained in DOR and AK with each 1 isolates ( 6,25 %) .Results of 'interpretative error'' is not found in Very major error. Minor error andMajor error Acinetobacter spp that exceeds the FDA criteria are AK is 12.7% and 10.9%. InPseudomonas spp Major error value that exceeds the FDA criteria are AK, CAS and DORwith 5.7%, 10.5% and 5.2%. The Minor error value that exceeds the FDA criteria are AK,CAS and CS are 31.5%, 21.1% and 10.5%. In Klebsiella spp, Major error on DOR, AK andCAS with 12.5%, 6:25% and 6:25%. The Minor error value on AK and DOR is 6:25% and6:25%.We conclude that MIC test can be used for microbial panresisten with the discdiffusion.] |