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[Salah satu penyakit infeksi mulut dengan prevalensi tinggi di Indonesia adalah kariesgigi. Hasil SKRT tahun 2009 mencatat bahwa 73% penduduk Indonesia menderitakaries gigi. Streptococcus mutans merupakan agen utama penyebab karies gigi. Telahdiketahui bahwa umbi binahong mempunyai sifat antibakteri. Oleh karena itu,penelitian ini bertujuan untuk membuktikan efek antibakteri infusum umbi binahongterhadap Streptococcus mutans secara in vitro. Infusum dibuat dalam 4 konsentrasiberbeda: 100%, 50%, 25% dan 10%. Uji dilusi dilakukan dengan media BHI cairdanTYS20B untuk mengetahui KHM dan KBM. Uji difusi menggunakan media BHAdan BHA darah untuk mengetahui besar zona hambatan. Dari uji dilusi, didapatkanKHM 25% dan KBM diatas 25%. Dari uji difusi pada media BHA, didapatkan besarzona hambatan: 1,375 mm (10%), 1,125 mm (25%), 0,75 mm (50%) dan 1,25 mm(100%). Dari uji difusi pada media BHA darah, didapatkan besar zona hambatan: 1,5mm (10%), 1,625 mm (25%), 1,5 mm (50%) dan 0,75 mm (100%). Bakteri S. mutanssensitif terhadap infusum umbi binahong., As one of the most common infectious oral diseases in Indonesia, dental caries hasrelatively high prevalence. Streptococcus mutans appears to be a prominent causativeagent of caries. Evidence shows that binahong rhizomes have antibacterial properties.Thus, the aim of the research is to prove whether infusion of binahong rhizomes iseffective as an antibacterial agent against Streptococcus mutans, in vitro. Theinfusion was made into four different concentrations: 100%, 50%, 25, and 10%. Todetermine minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC), dilution test was performed on BHI broth and TYS20Bmediums. As to measure the zone of inhibition, diffusion test was performed onBrain-Heart Agar and Brain-Heart-Blood Agar mediums. MIC is achieved on 25%concentration and the MBC is above 25%. Inhibitory zone results on BHA diffusiontest are: 1.375 mm (10%), 1.125 mm (25%), 0.75 mm (50%) and 1.25 mm (100%).While on BHB Agar medium: 1.5 mm (10%), 1.625 mm (25%), 1.5 mm (50%) and0.75 mm (100%). It is concluded that S. mutans is sensitive to binahong rhizomesinfusion.] |
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