Abstract
Belum ada prosedur baku sentrifugasi untuk pemisahan protein. Dilaporkan bahwa sentrifugasi 10.000 g dapat memisahkan protein saliva ?30 kDa.
Tujuan: Mengetahui pengaruh kecepatan sentrifugasi 7.000 g, 8.000 g, dan 9.000 g terhadap frekuensi kemunculan dan profil protein saliva ?30 kDa.
Metode: Profil protein supernatan saliva hasil sentrifugasi diuji dengan SDS-PAGE
Hasil: Frekuensi kemunculan protein ?30 kDa mengalami penurunan sesuai peningkatan kecepatan sentrifugasi. Terdapat perbedaan profil protein antara hasil sentrifugasi 7.000 g, 8.000 g, dan 9.000 g.
Kesimpulan: Kecepatan sentrifugasi 7.000 g, 8.000 g, dan 9.000 g berpengaruh terhadap frekuensi kemunculan dan profil protein ?30 kDa.
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There are no established standard operational procedure of centrifugation for protein separation. Centrifugation at 10.000 g separates salivary protein ?30 kDa.
Objective: To determine the effect of centrifugation at 7.000 g, 8.000 g, and 9.000 g on the frequency of salivary protein emergence and protein profile ?30 kDa.
Method: Salivary supernatant were analyzed with SDS-PAGE.
Results: Increased centrifugation speed resulted in decreased frequency of protein ?30 kDa. There are differences in the protein profiles between the results of each centrifugation.
Conclusion: Centrifugation at 7.000 g, 8.000 g,and 9.000 g influence frequency of salivary protein emergence and protein profiles ?30 kDa.
