ABSTRAK
Tirosinase merupakan suatu enzim yang mengkatalisis proses melanogenesisdalam pembentukan pigmen melanin. Produksi melanin yang berlebihan(hiperpigmentasi) dapat menyebabkan terjadinya penggelapan warna kulit.Pengontrolan produksi melanin dapat dilakukan dengan cara menghambataktivitas enzim tirosinase. Minyak biji markisa kuning diduga memiliki efekterhadap pencerahan kulit. Tujuan dari penelitian ini adalah untuk mengetahuipotensi penghambatan aktivitas tirosinase serta menentukan parameter kualitasminyak biji markisa kuning. Pengujian penghambatan tirosinase oleh minyak bijimarkisa kuning dilakukan dengan mengukur serapan L-dopakrom pada panjanggelombang 490 nm. Minyak biji markisa kuning memiliki nilai IC50 195,342μg/mL. Pengujian kinetika enzim melalui plot Lineweaver-Burk menunjukkanbahwa minyak biji markisa memiliki penghambatan kompetitif. Hasil penentuanparameter minyak biji markisa menunjukkan hasil bobot jenis 0,8912 g/cm3,indeks bias 1,472, bilangan asam 53,962 mgNaOH/g, bilangan penyabunan186,64 mgKOH/g, bilangan iodium 375,5 g I2/ 100 g minyak, bilangan hidroksil239,38 mg/g dan zat tidak tersabunkan 1,169, dan mengandung saponin, tanin,serta glikosida
ABSTRACTTyrosinase is an enzyme that catalyzes the process of melanogenesis which incase of excessive melanin production (hyperpigmentation) may causes darkeningof skin color. Melanin production can be controlled by the inhibition of tyrosinaseenzyme. Yellow passion fruit seeds was investigated had an effect on the skinwhitening. This research used to determined the potential inhibition of tyrosinaseactivity from yellow passion fruit seed oil and determined parameters of theyellow passion fruit seed oil. The inhibition of tyrosinase activity test from yellowpassion fruit seed oil was done by measuring the wavelength of dopachrome at490 nm. The test showed that yellow passion fruit seed oil had IC50 values of195.342 μg/mL. The inhibition kinetics analyzed by a Lineweaver-Burk plotindicated that yellow passion fruit seed oil was a noncompetitive inhibitor.Passion fruit seed oil had density of 0.8912 of g/cm3, refractive index of 1.472,acid value of 53.962 mgNaOH/g, saponification value 186.64 of mg/g, iodinevalue of 375.5 g I2/ 100 g oil, hydroxyl value of 239.38 mg/g, unsaponifiablematter of 1.169, and contained saponins, tannins, and glycosides |
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