Pengaruh perlakuan variasi medan listrik terhadap tingkat letal cell line MCF-7 (human breast cancer) in vitro dan pengukuran nilai kapasitansi sel = Effect of electric field treatment variation against lethal level cell line MCF-7 human breast cancer in vitro and measurement of cell capacitance value

Qolby Sabrina, author

Pengaruh perlakuan variasi medan listrik terhadap tingkat letal cell line MCF-7 (human breast cancer) in vitro dan pengukuran nilai kapasitansi sel = Effect of electric field treatment variation against lethal level cell line MCF-7 human breast cancer in vitro and measurement of cell capacitance value

Qolby Sabrina; Djarwani Soeharso Soejoko, supervisor; Warsito Purwo Taruno, supervisor; Mussadiq Musbach, examiner; Supriyanto Ardjo Pawiro, examiner; Prawito, examiner (Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, 2014)

Abstrak

[ABSTRAK

Electro Capacitive Cancer Therapy (ECCT) telah dikembangkan untuk terapi

kanker payudara dengan medan elektrostatik dari gelombang sinyal yang berasal

dari elektroda kapasitif. Tujuan dari penelitian ini adalah untuk mengetahui

pengaruh perubahan nilai beda potensial dan output gelombang sinyal yang

dihsilkan ECCT terhadap pertumbuhan sel kanker dan cidera sel yang

menyebabkan kematian sel. Dilakukan eksperimen in vitro menggunakan cell line

MCF-7 (kanker payudara manusia) selama 24, 48 dan 72 jam perlakuan. Kemudian

dilakukan perhitungan jumlah sel dengan hemocytometer dan pengukuran

kapasitansi sel sebelum dan sesudah diberikan perlakuan. Hasil eksperimen

menunjukkan bahwa ECCT standar sinyal kotak 18 Volt dan ECCT non standar

sinyal kotak 31.2 Volt dapat menghambat pertumbuhan sel dan hasil morfologi sel

tampak cidera yang mengindikasi adanya kematian, sedangkan ECCT standar

sinyal sinusoidal 18 Volt mampu menginduksi pertumbuhan sel sehingga

jumlahnya semakin banyak jika dilihat dari koefisien pertumbuhan yang tinggi.

Pengukuran nilai kapasitansi sel menunjukkan korelasi antara banyaknya jumlah

sel dengan besarnya nilai kapasitansi yang terukur. Peningkatan nilai kapasitansi

dapat menunjukkan penambahan aktifitas kelistrikan sel dan tingkat keganasan

dari sel kanker.

ABSTRACT

Electro Capacitive Cancer Therapy (ECCT) has been developed for breast cancer

therapy that generated electrostatic field from electrical wave in capacitive

electrode. The purpose of this research is to find out the effect of potential

difference and signal wave output ECCT to cancer cell growth and cell injury that

leads to lethal cell. In this study, in vitro experiment use MCF-7 cell line (human

breast cancer) during 24, 48 and 72 hours treatment and than measured the number

of cell with hemocytometer and value of capacitance after and before treatment.

The results showed that potential ECCT square signal wave standard 18 Volt and

square signal non-standard 31.2 Volt can inhibit cancer cell growth and cell

morphology results seem to indicate the existence of injury deaths. While, from

growth coefficient, ECCT sinusoidal signal wave standard 18 Volt can increase of

cancer cell growth. Measurement of cell capacitance values showed correlation

between the number of cells with the value of the measured capacitance. Increase

of capacitance indicate of high activity of cancer cell and showed the malignancy

of cancer cell levels.;Electro Capacitive Cancer Therapy (ECCT) has been developed for breast cancer