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Pengaruh medan listrik terhadap kanker darah uji sel in vitro sel raji dan sel limfosit = Electric field effect on blood cancer in vitro test on raji cell and lymphocyte cell

Ikrimah; Warsito Purwo Taruno, supervisor; Djarwani Soeharso Soejoko, supervisor; Mussadiq Musbach, examiner; Supriyanto Ardjo Pawiro, examiner; Prawito, examiner ([Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, Universitas Indonesia], 2014)

 Abstrak

[ABSTRAK
Terapi kanker mennggunakan medan listrik telah dikembangkan sebelumnya. Terapi ini bekerja berdasarkan prinsip polarisasi sel kanker yang sedang membelah ketika diberi medan listrik dalam waktu tertentu. Dalam penelitian ini telah dilakukan uji in-vitro pada sel kanker raji dan sel normal limfosit. Sel ditanam pada 96 microplate kemudian diberi perlakuan input medan listrik dengan variasi frekuensi (250 Hz & 100 kHz), tegangan (20V, 30V, 50V), jenis sinyal (kotak dan sinusoidal) dana lama perlakuan (0 jam, 48 jam, 96 jam dan 144 jam).
Setelah sel diberi perlakuan, jumlah sel dihitung menggunakan Hemocytometer untuk morfologi sel dan MTT untuk pewarnaan sel. Hasil penelitian menunjukkan
bahwa medan listrik berpengaruh pada pertumbuhan sel (menginduksi). Pada sel limfosit pengaruh medan listrik dapat terlihat secara signifikan pada jam ke-48.
Akan tetapi, pada sel raji pengaruh medan listrik baru dapat terlihat setelah perlakuan 96 jam. Input medan listrik optimal untuk dapat memberi efek letal
(cidera) pada sel raji tanpa berpangaruh toxic pada sel normal yaitu medan listrik sinyal kotak 20 volt dengan frekuensi 100 kHz.

ABSTRACT
Cancer therapy using electric fields have been developed previously. It works based on polarized division cancer cells when exposed by electric field in a certain
time. In this study, raji and lymphocyte cells in-vitro exposed to an external electrical field has been done. Cultured cells on 96 microplate then given electric
field treatment with variety input of frequency (250 Hz and 100 kHz), voltage (20 V, 30 V, and 50 V), the signal type (square and sine), and time treatment (0 hours,
48 hours, 96 hours, and 144 hours). Calculation of number cells method using Hemocytometer and MTT for cell morphology and cell staining absorption, respectively. The results show that electric field can induce cells. The influence of an electric field in lymphocyte cells show significant effect after 48-hours treatment, yet raji cells can only be seen after 96-hours treatment. The optimal treatment of the electric field to be able provide lethal effects in raji cells without toxic effect in normal cells is input square signal 20 Volt and 100 kHz.;Cancer therapy using electric fields have been developed previously. It works based on polarized division cancer cells when exposed by electric field in a certain
time. In this study, raji and lymphocyte cells in-vitro exposed to an external electrical field has been done. Cultured cells on 96 microplate then given electric
field treatment with variety input of frequency (250 Hz and 100 kHz), voltage (20 V, 30 V, and 50 V), the signal type (square and sine), and time treatment (0 hours,
48 hours, 96 hours, and 144 hours). Calculation of number cells method using Hemocytometer and MTT for cell morphology and cell staining absorption, respectively. The results show that electric field can induce cells. The influence of an electric field in lymphocyte cells show significant effect after 48-hours treatment, yet raji cells can only be seen after 96-hours treatment. The optimal treatment of the electric field to be able provide lethal effects in raji cells without toxic effect in normal cells is input square signal 20 Volt and 100 kHz., Cancer therapy using electric fields have been developed previously. It works based on polarized division cancer cells when exposed by electric field in a certain
time. In this study, raji and lymphocyte cells in-vitro exposed to an external electrical field has been done. Cultured cells on 96 microplate then given electric
field treatment with variety input of frequency (250 Hz and 100 kHz), voltage (20 V, 30 V, and 50 V), the signal type (square and sine), and time treatment (0 hours,
48 hours, 96 hours, and 144 hours). Calculation of number cells method using Hemocytometer and MTT for cell morphology and cell staining absorption, respectively. The results show that electric field can induce cells. The influence of an electric field in lymphocyte cells show significant effect after 48-hours treatment, yet raji cells can only be seen after 96-hours treatment. The optimal treatment of the electric field to be able provide lethal effects in raji cells without toxic effect in normal cells is input square signal 20 Volt and 100 kHz.]

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 Metadata

No. Panggil : T41410
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Subjek :
Penerbitan : Depok: [Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Indonesia, Universitas Indonesia], 2014
Program Studi :
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : xiii, 45 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, lantai 3
  • Ketersediaan
  • Ulasan
No. Panggil No. Barkod Ketersediaan
T41410 15-17-683210710 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20389321