[ABSTRAK
Aktivitas bakteri merupakan salah satu penyebab jerawat. Rimpang kencur(Kaempferia galanga L.) memiliki aktivitas antibakteri yang berasal dari senyawaetil p-metoksi sinamat (EPMS). Tujuan penelitian ini adalah untuk mengetahuiaktivitas antibakteri dari EPMS ekstrak rimpang kencur terhadap P.acne, S.aureusdan S.epidermidis serta stabilitas fisik krim dan keamanan krim anti jerawat.Kristal EPMS diperoleh melalui proses maserasi dengan pelarut heksan, lalupemurnian dan identifikasi karakteristik kristal EPMS. Uji aktivitas antibakteridan konsentrasi hambat minimum (KHM) pada konsentrasi EPMS 0,3; 0,6; 1,2dan 2,4% dengan metode difusi cakram dan dilusi cair. Hasil menunjukkan semuakonsentrasi EPMS memiliki aktivitas antibakteri secara signifikan (p<0,01)dengan zona jernih secara berturut-turut terhadap P.acne (9,00; 11,50; 14,50;16,00 mm), S.aureus (9,00; 11,50; 16,50; 22,00 mm) dan S.epidermidis (10,50;12,50; 20,50; 27,00 mm). Senyawa EPMS dengan konsentrasi 0,6; 1,2 dan 2,4 %terbukti memiliki konsentrasi hambat minimum (KHM) terhadap bakteri P.acne,sedangkan pada S.aureus dan S.epidermidis pada konsentrasi 1,2 dan 2,4%.Berdasarkan hasil evaluasi stabilitas fisik, krim EPMS 1,2% memiliki stabilitasfisik baik hingga akhir penelitian. Dari hasil uji keamanan (pacth test) pada 12subjek tidak terjadi iritasi alergi, sehingga krim EPMS 1,2% aman digunakandalam sediaan topikal.
ABSTRACT
An activity of certain bacterias is one of the causes of acne. Rimpang kencur(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxycinnamate (EPMC). The purpose of this research is to find out theactivities of antibacterias using EPMC rimpang kencur extracts against P.acne,S.aureus and S.epidermidis with the physical stability of the cream and its safetyuse as an anti acne cream. The EPMC crystals are obtained through the process ofmaceration using hexane solvent, purification and identification of thecharacteristic of EPMC crystals. The activity test of the antibacterial and theminimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% wasdone using disk diffusion method and broth dilution test. The result shows that allEPMC concentration has significant antibanterial activity (p<0,01) respectivelygaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to haveminimum inhibitory concentration (MIC) against P.acne bacterias, while on theS.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based onthe results of the evaluation on the physical stability, EPMC 1,2% cream has agood physical stability until the final research. From the results of safety use(pacth test) on 12 subjects there were no evidence of allergic irritation, thereforecream EPMC 1,2% is safe to be used in topical preparation;An activity of certain bacterias is one of the causes of acne. Rimpang kencur(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxycinnamate (EPMC). The purpose of this research is to find out theactivities of antibacterias using EPMC rimpang kencur extracts against P.acne,S.aureus and S.epidermidis with the physical stability of the cream and its safetyuse as an anti acne cream. The EPMC crystals are obtained through the process ofmaceration using hexane solvent, purification and identification of thecharacteristic of EPMC crystals. The activity test of the antibacterial and theminimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% wasdone using disk diffusion method and broth dilution test. The result shows that allEPMC concentration has significant antibanterial activity (p<0,01) respectivelygaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to haveminimum inhibitory concentration (MIC) against P.acne bacterias, while on theS.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based onthe results of the evaluation on the physical stability, EPMC 1,2% cream has agood physical stability until the final research. From the results of safety use(pacth test) on 12 subjects there were no evidence of allergic irritation, thereforecream EPMC 1,2% is safe to be used in topical preparation, An activity of certain bacterias is one of the causes of acne. Rimpang kencur(Kaempferiagalanga L.) has an antibacterial agent from compound ethyl pmethoxycinnamate (EPMC). The purpose of this research is to find out theactivities of antibacterias using EPMC rimpang kencur extracts against P.acne,S.aureus and S.epidermidis with the physical stability of the cream and its safetyuse as an anti acne cream. The EPMC crystals are obtained through the process ofmaceration using hexane solvent, purification and identification of thecharacteristic of EPMC crystals. The activity test of the antibacterial and theminimum inhibitory concentration (MIC) of EPMC are 0,3; 0,6; 1,2 and 2,4% wasdone using disk diffusion method and broth dilution test. The result shows that allEPMC concentration has significant antibanterial activity (p<0,01) respectivelygaining clear zone against P.acne (9,00; 11,50; 14,50; 16,00 mm), S.aureus (9,00;11,50; 16,50; 22,00 mm) and S.epidermidis (10,50; 12,50; 20,50; 27,00 mm).EPMC compound with the 0,6; 1,2 dan 2,4 % concentration is proven to haveminimum inhibitory concentration (MIC) against P.acne bacterias, while on theS.aureus dan S.epidermidis reaches up to 1,2 dan 2,4% concentration. Based onthe results of the evaluation on the physical stability, EPMC 1,2% cream has agood physical stability until the final research. From the results of safety use(pacth test) on 12 subjects there were no evidence of allergic irritation, thereforecream EPMC 1,2% is safe to be used in topical preparation] |
T41554-Ika Maruya Kusuma.pdf :: Unduh