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Perbandingan metode fenotipik terhadap metode genotipik untuk deteksi methicillin resistant staphylococcus aureus (MRSA) = Comparison between phenotypic method to genotypic method to detect methicillin resistant staphylococcus aureus (MRSA)

Arleen N. Suryatenggara; July Kumalawati, supervisor; Dodi Safari, supervisor; Rustadi Sosrosumihardjo, examiner; Ninik Sukartini, examiner; Yusra, co-promotor (Fakultas Kedokteran Universitas Indonesia, 2014)

 Abstrak

[ABSTRAK
Infeksi yang disebabkan oleh methicillin-resistant Staphylococcus aureus
(MRSA) telah menyebabkan beban mortalitas dan morbiditas yang bermakna.
Mengingat hal tersebut, sangat penting untuk dapat mendeteksi MRSA dengan
cepat dan akurat. Saat ini deteksi MRSA dapat dilakukan dengan dua cara, yaitu
metode fenotipik dan genotipik. Pada penelitian ini, metode fenotipik dilakukan
dengan uji kepekaan antibiotik menggunakan oksasilin dan sefoksitin, sementara
metode genotipik dilakukan dengan polymerase chain reaction (PCR) gen nuc
dan mecA.
Gen nuc merupakan penanda genetik S. aureus, sedangkan gen mecA
adalah gen yang mengkode penicillin-binding protein 2a (PBP2a). Protein ini
memiliki afinitas rendah terhadap antibiotik β-laktam, sehingga menyebabkan
resistensi terhadap antibiotik seperti metisilin, oksasilin, dan sefoksitin. Penelitian
ini bertujuan untuk membandingkan metode fenotipik terhadap metode genotipik
yang merupakan baku emas dalam mendeteksi MRSA.
Sebanyak 136 isolat S. aureus diikutsertakan dalam penelitian ini.
Dilakukan PCR untuk mengamplifikasi gen nuc dan mecA dengan hasil: 37
sampel terdeteksi sebagai MRSA (nuc+, mecA+), 96 sampel sebagai methicillinsensitive
Staphylococcus aureus atau MSSA (nuc+, mecA-), and 3 sampel sebagai
bukan S. aureus (nuc-). Persentase MRSA yang dideteksi dengan metode
genotipik adalah sebesar 27,8%.
Deteksi MRSA dengan metode fenotipik dilakukan dengan uji kepekaan
antibiotik menggunakan oksasilin dan sefoksitin. Tidak terdapat perbedaan hasil
uji kepekaan antara kedua antibiotik tersebut. Secara keseluruhan, hasil deteksi
MRSA dengan metode fenotipik konsisten dengan metode genotipik, dengan
dideteksinya MRSA sebesar 27,8%. Hal tersebut mengartikan bahwa sensitivitas
dan spesifisitas metode fenotipik terhadap metode genotipik adalah sebesar 100%.

ABSTRACT
Methicillin-resistant Staphylococcus aureus (MRSA) infection has caused
significant morbidity and mortality burden. Therefore, detecting MRSA
accurately as early as possible is very important. There are two methods used in
detecting MRSA, which are phenotypic and genotypic methods. In this study,
phenotypic method was done by antibiotic susceptibility test using oxacillin and
cefoxitin, while genytopic method was carried out by amplifying nuc and mecA
gene with polymerase chain reaction (PCR).
Nuc gene is a genetic marker for S. aureus, and mecA gene is responsible
in the coding of penicillin-binding protein 2a (PBP2a). This protein has a low
affinity to β-lactam antibiotics, thus causing antibiotic resistance to the antibiotics,
such as methicillin, oxacillin, and cefoxitin. This study was aimed to compare
phenotycipic method to genotypic method as the gold standard, to detect MRSA.
There were 136 S. aureus isolates included in this study. PCR to amplify
nuc and mecA gene was conducted with the results of the following: 37 samples
detected as MRSA (nuc+, mecA+), 96 samples as methicillin-sensitive
Staphylococcus aureus or MSSA (nuc+, mecA-), and 3 samples as non-S. aureus
(nuc-). The percentage of MRSA detected by genotypic method was 27,8%.
The detection of MRSA through the phenotypic method was done by
antibiotic susceptibility test using oxacillin and cefoxitin. Susceptibility test
between these antibiotics showed no difference in result. In general, the result of
phenotypic method was consistent to the results from the genotypic method, by
detecting 27,8% MRSA. Therefore, the sensitivity and specificity of phenotypic
method compared to the genotypic method were 100%.;Methicillin-resistant Staphylococcus aureus (MRSA) infection has caused
significant morbidity and mortality burden. Therefore, detecting MRSA
accurately as early as possible is very important. There are two methods used in
detecting MRSA, which are phenotypic and genotypic methods. In this study,
phenotypic method was done by antibiotic susceptibility test using oxacillin and
cefoxitin, while genytopic method was carried out by amplifying nuc and mecA
gene with polymerase chain reaction (PCR).
Nuc gene is a genetic marker for S. aureus, and mecA gene is responsible
in the coding of penicillin-binding protein 2a (PBP2a). This protein has a low
affinity to β-lactam antibiotics, thus causing antibiotic resistance to the antibiotics,
such as methicillin, oxacillin, and cefoxitin. This study was aimed to compare
phenotycipic method to genotypic method as the gold standard, to detect MRSA.
There were 136 S. aureus isolates included in this study. PCR to amplify
nuc and mecA gene was conducted with the results of the following: 37 samples
detected as MRSA (nuc+, mecA+), 96 samples as methicillin-sensitive
Staphylococcus aureus or MSSA (nuc+, mecA-), and 3 samples as non-S. aureus
(nuc-). The percentage of MRSA detected by genotypic method was 27,8%.
The detection of MRSA through the phenotypic method was done by
antibiotic susceptibility test using oxacillin and cefoxitin. Susceptibility test
between these antibiotics showed no difference in result. In general, the result of
phenotypic method was consistent to the results from the genotypic method, by
detecting 27,8% MRSA. Therefore, the sensitivity and specificity of phenotypic
method compared to the genotypic method were 100%., Methicillin-resistant Staphylococcus aureus (MRSA) infection has caused
significant morbidity and mortality burden. Therefore, detecting MRSA
accurately as early as possible is very important. There are two methods used in
detecting MRSA, which are phenotypic and genotypic methods. In this study,
phenotypic method was done by antibiotic susceptibility test using oxacillin and
cefoxitin, while genytopic method was carried out by amplifying nuc and mecA
gene with polymerase chain reaction (PCR).
Nuc gene is a genetic marker for S. aureus, and mecA gene is responsible
in the coding of penicillin-binding protein 2a (PBP2a). This protein has a low
affinity to β-lactam antibiotics, thus causing antibiotic resistance to the antibiotics,
such as methicillin, oxacillin, and cefoxitin. This study was aimed to compare
phenotycipic method to genotypic method as the gold standard, to detect MRSA.
There were 136 S. aureus isolates included in this study. PCR to amplify
nuc and mecA gene was conducted with the results of the following: 37 samples
detected as MRSA (nuc+, mecA+), 96 samples as methicillin-sensitive
Staphylococcus aureus or MSSA (nuc+, mecA-), and 3 samples as non-S. aureus
(nuc-). The percentage of MRSA detected by genotypic method was 27,8%.
The detection of MRSA through the phenotypic method was done by
antibiotic susceptibility test using oxacillin and cefoxitin. Susceptibility test
between these antibiotics showed no difference in result. In general, the result of
phenotypic method was consistent to the results from the genotypic method, by
detecting 27,8% MRSA. Therefore, the sensitivity and specificity of phenotypic
method compared to the genotypic method were 100%.]

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No. Panggil : T-Pdf
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Subjek :
Penerbitan : Jakarta: Fakultas Kedokteran Universitas Indonesia, 2014
Program Studi :
Bahasa : ind
Sumber Pengatalogan :
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xiii, 43 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI
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T-Pdf 15-17-390760246 TERSEDIA
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