[ABSTRAK
Platelet Rich Plasma (PRP) diketahui dapat digunakan untukmenggantikan serum hewan sebagai suplemen media kultur sel meskipun sudahkadaluwarsa sampai tiga minggu. Berbagai metode pemrosesan PRP sebelumdigunakan menyebabkan hasil yang tidak konsisten pada kultur sel. Hal tersebutdiduga karena kadar faktor pertumbuhan dan jumlah trombosit yang bervariasipada PRP. Penelitian ini bertujuan untuk mengetahui cara pemprosesan PRP yangmemberikan hasil kadar faktor pertumbuhan tertinggi dan mengetahui hubungankadar faktor pertumbuhan dan jumlah trombosit sebelum dan sesudahkadaluwarsa pada PRP dari Unit Transfusi Darah (UTD) PMI. Enam kantong PRPsebelum dan sesudah kadaluwarsa UTD PMI diberi perlakuan dengan aktivasitrombin dan cara lisis satu, dua dan tiga kali simpan beku (dibekukan pada -20οCselama 30 menit dan dicairkan pada suhu ruang selama 15 menit). Kadar faktorpertumbuhan TGF-β1, PDGF AB, EGF, IGF-1, VEGF diukur menggunakanSigma ELISA Kit serta Hematology Anlyzer Sysmex XN-1000 untuk menghitungjumlah trombosit. Hasil penelitian menunjukkan jumlah trombosit semakinmenurun secara perlahan dengan semakin banyak jumlah simpan beku. KadarTGFβ-1 dan EGF tertinggi didapatkan pada perlakuan aktivasi trombin, PDGFAB dan IGF-1 pada tiga kali simpan beku dan VEGF pada satu kali simpan beku.Tidak terdapat pengaruh perlakuan simpan beku terhadap kadar faktorpertumbuhan kecuali perlakuan aktivasi trombin pada TGFβ-1 dan perlakuansimpan beku pada VEGF. Tidak ada pengaruh yang signifikan jumlah trombositdan kadar faktor pertumbuhan dari PRP sebelum dan sesudah Kadaluwarsa dariPMI.
ABSTRACT
Platelet Rich Plasma (PRP) known to be used to replace animal serum as a cellculture supplement media even though out dated up to three weeks. Variousprocessing methods PRP before use, causing inconsistent results in cell culture.This is presumably because the levels of the growth factor and platelet counts inPRP varied. This study aims to determine how the PRP processing that yields thehighest levels of growth factors and growth factor levels determine relationshipsand platelet counts before and after out dated in PRP from Unit of BloodTransfusion PMI. Six bags of PRP before and after out dated from Unit of BloodTransfusion PMI were treated with thrombin activation and lysis method withone, two and three times freeze thaw (freeze 30 minutes in -20οC and thaw 15minutes in room temperature). Levels of the growth factor TGF- β1, PDGF AB,EGF, IGF-1, VEGF were measured using ELISA Kit and Sigma Anlyzer SysmexHematology XN -1000 to calculate the number of platelets. The results show theplatelets number decreases slowly with increasing numbers freeze thaw cycle.Levels of TGFβ-1 and EGF highest activation was found in the treatment ofthrombin, PDGF AB and IGF-1 at the store three times cycle freeze thaw andVEGF in one cycle freeze thaw. There was no effect of treatment on the freezethaw cycle and growth factor levels unless treatment thrombin activation onTGFβ-1 and VEGF treatment on the freeze thaw cycle. No significant effect of theplatelets number and growth factors levels before and after out dated PRP fromUnit of Blood Transfusion PMI;Platelet Rich Plasma (PRP) known to be used to replace animal serum as a cellculture supplement media even though out dated up to three weeks. Variousprocessing methods PRP before use, causing inconsistent results in cell culture.This is presumably because the levels of the growth factor and platelet counts inPRP varied. This study aims to determine how the PRP processing that yields thehighest levels of growth factors and growth factor levels determine relationshipsand platelet counts before and after out dated in PRP from Unit of BloodTransfusion PMI. Six bags of PRP before and after out dated from Unit of BloodTransfusion PMI were treated with thrombin activation and lysis method withone, two and three times freeze thaw (freeze 30 minutes in -20οC and thaw 15minutes in room temperature). Levels of the growth factor TGF- β1, PDGF AB,EGF, IGF-1, VEGF were measured using ELISA Kit and Sigma Anlyzer SysmexHematology XN -1000 to calculate the number of platelets. The results show theplatelets number decreases slowly with increasing numbers freeze thaw cycle.Levels of TGFβ-1 and EGF highest activation was found in the treatment ofthrombin, PDGF AB and IGF-1 at the store three times cycle freeze thaw andVEGF in one cycle freeze thaw. There was no effect of treatment on the freezethaw cycle and growth factor levels unless treatment thrombin activation onTGFβ-1 and VEGF treatment on the freeze thaw cycle. No significant effect of theplatelets number and growth factors levels before and after out dated PRP fromUnit of Blood Transfusion PMI, Platelet Rich Plasma (PRP) known to be used to replace animal serum as a cellculture supplement media even though out dated up to three weeks. Variousprocessing methods PRP before use, causing inconsistent results in cell culture.This is presumably because the levels of the growth factor and platelet counts inPRP varied. This study aims to determine how the PRP processing that yields thehighest levels of growth factors and growth factor levels determine relationshipsand platelet counts before and after out dated in PRP from Unit of BloodTransfusion PMI. Six bags of PRP before and after out dated from Unit of BloodTransfusion PMI were treated with thrombin activation and lysis method withone, two and three times freeze thaw (freeze 30 minutes in -20οC and thaw 15minutes in room temperature). Levels of the growth factor TGF- β1, PDGF AB,EGF, IGF-1, VEGF were measured using ELISA Kit and Sigma Anlyzer SysmexHematology XN -1000 to calculate the number of platelets. The results show theplatelets number decreases slowly with increasing numbers freeze thaw cycle.Levels of TGFβ-1 and EGF highest activation was found in the treatment ofthrombin, PDGF AB and IGF-1 at the store three times cycle freeze thaw andVEGF in one cycle freeze thaw. There was no effect of treatment on the freezethaw cycle and growth factor levels unless treatment thrombin activation onTGFβ-1 and VEGF treatment on the freeze thaw cycle. No significant effect of theplatelets number and growth factors levels before and after out dated PRP fromUnit of Blood Transfusion PMI] |
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