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Gambaran status metilasi gen promoter methylguanine deoksiribonucleic acid methyltransferase pada astrositoma dan faktor-faktor yang mempengaruhinya = Methylation status of the methylguanine deoksiribonucleic acid methyltransferase gene promoter among patients with astrocytomas

Hadio Ali Khazatsin; Tiara Anindhita, supervisor; Esti D.S. Soetrisno B., supervisor; Joedo Prihartono, supervisor; Teguh Asaad Suhatno Ranakusuma, examiner; Santoso Cornain, examiner; Yetty Ramli, examiner (Fakultas Kedokteran Universitas Indonesia, 2014)

 Abstrak

[Latar belakang—Metilasi dari gen promoter O6-methylguanine-DNA methyltransferase (MGMT) adalah salah satu faktor yang berperan pada karsinogenesis dan berkembang menjadi marker dalam menilai progresivisitas dan respons terapi astrositoma. Tujuan—Untuk mendapatkan gambaran frekuensi status metilasi gen promoter MGMT pada pasien astrositoma menggunakan methylation specific polymerase chain reaction (MS-PCR) dan methylation specific high resolution melting (MS-HRM). Metode—Dilakukan pengumpulan data klinis, imajing dan blok parafin jaringan astrositoma di RSCM dalam kurun waktu 2008-2012. Status metilasi gen promoter MGMT dianalisis menggunakan MS-PCR dan MS-HRM serta dihubungkan dengan berbagai faktor prognostik klinis. Penelitian ini adalah penelitian potong-lintang. Hasil— Didapatkan 13 sampel yang terdiri dari 7 astrositoma derajat rendah dan 6 astrositoma derajat tinggi. Metilasi gen promoter MGMT didapatkan pada 1/13 sampel astrositoma dengan MS-PCR dan 4/13 sampel dengan MS-HRM yang seluruhnya adalah astrositoma derajat rendah. Terdapat perbedaan yang bermakna antara status metilasi gen promoter MGMT dengan derajat keganasan astrositoma yaitu astrositoma derajat rendah 4/7 sampel, tanpa ditemukan pada astrositoma derajat tinggi (p=0.049) sedangkan faktor lain seperti usia, jenis kelamin, karnofsky performance scale (KPS), lokasi astrositoma dan derajat WHO tidak terdapat perbedaan yang bermakna (p= 1,000; p= 0,657; p= 0,354; p= 0,538). Simpulan—Penelitian saat ini menunjukkan frekuensi status metilasi gen promoter MGMT pada astrositoma sedikit berbeda dengan berbagai penelitian lain sebelumnya yaitu hipermetilasi hanya terjadi pada astrositoma derajat rendah. Penelitian ini merupakan penelitian pertama di Indonesia yang melaporkan gambaran status metilasi gen promoter MGMT pada pasien astrositoma.

Background—Astrocytoma is the most common primary central nervous system tumor with difficult management as it requires a combination of surgery, chemotherapy and radiotherapy. This multimodal approach increases patients survival rate significantly, however chemotherapy resistance is now commonly seen. One of the potential causes of chemotherapy resistance is the epigenetic factors from O6 methylguanine-DNAmethyltransferase (MGMT) gene. MGMT gene has role in DNA repair and also have a protective effect against exyogen and endogeneous alkylating agent. The methylation of MGMT gene promoter leads to the decrease of MGMT protein, attenuating its function. Therefore, the methylation status of MGMT gene promoter can act as an indicator for astrocytomas progresivity and treatment aggressiveness. Objective—To determine the frequency of MGMT gene promoter methylation among patients with astrocytomas using methylation specific polymerase chain reaction (MS-PCR) and methylation sensitive high resolution melting (MS-HRM). Methods—Clinical data, imaging and parafin blocks from astrocytoma patients were collected in RSCM from 2008-2012. The methylation status of MGMT gene promoter was confirmed using MS-PCR and MS-HRM. This is cross-sectional study. Results—The total of 13 samples collected including 7 low-grade and 6 high-grade astrocytomas. The MGMT gene promoter was methylated in 1/13 cases using MS-PCR and 4/13 cases using MS-HRM. All methylated cases were low-grade astrocytoma. There was significant association between methylation status of MGMT gene promoter with degree of malignancy which is 4/7 samples hypermethylated in low-grade with no hypermethylation in high-grade astrocytomas (p=0.049). While other factors like age, sex, KPS and astrocytomas location have no significant association (p= 1,000; p= 0,657; p= 0,354; p= 0,538). Conclusions— The present study showed difference of methylation of MGMT gene promoter in astrocytomas with others studies which is hypermethylated MGMT only found in low grade astrocytomas. Our study was the first to report the frequency of MGMT promoter methylation among Indonesian astrocytoma patients., Background—Astrocytoma is the most common primary central nervous system tumor with
difficult management as it requires a combination of surgery, chemotherapy and
radiotherapy. This multimodal approach increases patients survival rate significantly,
however chemotherapy resistance is now commonly seen. One of the potential causes of
chemotherapy resistance is the epigenetic factors from O6 methylguanine-DNAmethyltransferase
(MGMT) gene. MGMT gene has role in DNA repair and also have a
protective effect against exyogen and endogeneous alkylating agent. The methylation of
MGMT gene promoter leads to the decrease of MGMT protein, attenuating its function.
Therefore, the methylation status of MGMT gene promoter can act as an indicator for
astrocytomas progresivity and treatment aggressiveness.
Objective—To determine the frequency of MGMT gene promoter methylation among
patients with astrocytomas using methylation specific polymerase chain reaction (MS-PCR)
and methylation sensitive high resolution melting (MS-HRM).
Methods—Clinical data, imaging and parafin blocks from astrocytoma patients were
collected in RSCM from 2008-2012. The methylation status of MGMT gene promoter was
confirmed using MS-PCR and MS-HRM. This is cross-sectional study.
Results—The total of 13 samples collected including 7 low-grade and 6 high-grade
astrocytomas. The MGMT gene promoter was methylated in 1/13 cases using MS-PCR and
4/13 cases using MS-HRM. All methylated cases were low-grade astrocytoma. There was
significant association between methylation status of MGMT gene promoter with degree of
malignancy which is 4/7 samples hypermethylated in low-grade with no hypermethylation in
high-grade astrocytomas (p=0.049). While other factors like age, sex, KPS and astrocytomas
location have no significant association (p= 1,000; p= 0,657; p= 0,354; p= 0,538).
Conclusions— The present study showed difference of methylation of MGMT gene
promoter in astrocytomas with others studies which is hypermethylated MGMT only found in
low grade astrocytomas. Our study was the first to report the frequency of MGMT promoter
methylation among Indonesian astrocytoma patients.]

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 Metadata

No. Panggil : T58021
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Subjek :
Penerbitan : Jakarta: Fakultas Kedokteran Universitas Indonesia, 2014
Program Studi :
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : unmediated ; computer
Tipe Carrier : volume ; online resource
Deskripsi Fisik : xvi, 37 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
  • Ketersediaan
  • Ulasan
No. Panggil No. Barkod Ketersediaan
T58021 15-24-11349454 TERSEDIA
Ulasan:
Tidak ada ulasan pada koleksi ini: 20391115