[Toluena dijumpai tersebar di lingkungan kita. Manusia terpapar logam ini dari berbagai sumber seperti udara, air, tanah dan makanan yang terkontaminasi.Terdapat beberapa penelitian yang menunjukkan bahwa toluena menyebabkan stres oksidatif dengan meningkatkan pembentukan reactive oxygen species dan menurunkan sistem anti-oksidan. Peroksidasi lipid meningkat karena terganggunya keseimbangan oksidan dan anti-oksidan, yang diukur dengan kadarmalondialdehyde. Penelitian ini bertujuan mengetahui pengaruh pemberian berbagai pajanan toluena terhadap peroksidasi lipid. Tiga puluh ekor tikus wistar jantan dengan berat badan 200-250 gram dibagi dalam lima kelompok. Kelompok I sebagai kontrol, kelompok II sampai V berturut-turut mendapat toluena secara inhalasi, dosis 12.5 ppm, 25 ppm, 50 ppm,dan 100 ppm, yang dipajan selama 4 jam/hari. Setelah 14 hari berturut-turut, dilakukan pengukuran kadar malondialdehyde plasma dan darah tepi. Peningkatan kadar malondialdehyde pada kelompok IV, secara statistik tidak bermakna (p=0,118). Peningkatan tersebut sejalan dengan peningkatan pajanantoluena yang diberikan, kecuali kelompok V. Beberapa hasil darah tepi, yang tidak bermakna secara statistik yaitu HB, Hematokrit, leukosit, neutrofil, limfosit dan monosit. Sedangkan eritrosit, VER, HER, KHER, trombosit, dan eosinofilsecara statistic menunjukkan adanya perbedaan bermakna.
Toulene can be found in our environment. Humans are exposed to these metalfrom various sources such as air, water, soil, and contaminated food. There areseveral studies that show that toluene causes oxidative stress by increasing theformation of reactive oxygen species and decreasing the anti-oxidant system.Increased lipid peroxidation are due to the disruption of the balance of oxidantsand antioxidants, as measured by levels of malondialdehyde. This study aims todetermine the effect of various exposure of toluene towards lipid peroxidation.Thirty male wistar rats weighing 200-250 grams are divided into five groups.Group I as a control variable, group II to group V each gets toluene throughinhalaton, with 12.5 ppm, 25 ppm, 50 ppm, dan 100 ppm doses, which areexposed for 4 hours/day. After 14 consecutive days, a measurement of the levelsof malondialdehyde plasma and peripheral blood is done.Increase in levels of malondialdehyde in group IV is not statistically significant(p=0,118). This increase is in line with the increase of given toluene exposure,except for group V. Some of the results of peripheral blood, which was notstatistically significant HB, hematocrit, leukocytes, neutrophil, lymphocytes andmonocytes. While erythrocytes, MCV, MCH, MCHC, platelets and eosinophilsstatically shows significant difference., Toulene can be found in our environment. Humans are exposed to these metalfrom various sources such as air, water, soil, and contaminated food. There areseveral studies that show that toluene causes oxidative stress by increasing theformation of reactive oxygen species and decreasing the anti-oxidant system.Increased lipid peroxidation are due to the disruption of the balance of oxidantsand antioxidants, as measured by levels of malondialdehyde. This study aims todetermine the effect of various exposure of toluene towards lipid peroxidation.Thirty male wistar rats weighing 200-250 grams are divided into five groups.Group I as a control variable, group II to group V each gets toluene throughinhalaton, with 12.5 ppm, 25 ppm, 50 ppm, dan 100 ppm doses, which areexposed for 4 hours/day. After 14 consecutive days, a measurement of the levelsof malondialdehyde plasma and peripheral blood is done.Increase in levels of malondialdehyde in group IV is not statistically significant(p=0,118). This increase is in line with the increase of given toluene exposure,except for group V. Some of the results of peripheral blood, which was notstatistically significant HB, hematocrit, leukocytes, neutrophil, lymphocytes andmonocytes. While erythrocytes, MCV, MCH, MCHC, platelets and eosinophilsstatically shows significant difference.] |
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