[Latar belakang : Toluena merupakan zat pelarut sering digunakan di berbagaiindustri seperti dalam pembuatan cat, lem dan lainnya. Toluena mempunyai sifatlipofilik dan memberikan efek toksik ke beberapa organ seperti sistem saraf pusat.Pada tahap biomolekuler, toluena merubah struktur lipid pada membran sel,sehingga terjadi peningkatan kadar MDA plasma dan jaringan. Pada Sistem SarafPusat, toluena bisa melewati sawar otak dan menyebabkan gangguan padaserebelum otak sehingga dapat meningkatkan kadar MDA serta terjadi perubahanstruktur pada dinding sel astrosit.Metode : Untuk mengetahui efek pajanan toluena selama 14 hari dengan dosisdibawah nilai ambang pada organ serebelum otak, dan dilakukan pemeriksaankadar MDA serebelum otak, serta kerusakan dari sel Astrosit, menggunakan limakelompok tikus jenis Wistar jantan dengan pajanan sebesar 1,6 ml; 3,2 ml; 6,4ml; 12,8 ml; dan kelompok kontrol tanpa pajanan.Hasil: Analisis uji nilai kadar MDA serebelum otak menggunakan One WayAnova dengan hasil tidak ada perbedaan rerata (p=0.133) antar kelompok pajanandengan kelompok kontrol, dan analisis jumlah sel Astrosit dengan menggunakanOne Way Anova didapatkan (p=0,310) dengan hasil tidak ada perbedaan antarkelompok pajanan.Kesimpulan : Tidak ada perbedaan rerata pada kelompok pajanan pada nilai MDAserebelum Otak maupun jumlah Sel Astrosit yang terpajan toluena dengan dosisdibawah nilai ambang.
Backgrounds : Toluene is a solvent commonly used in various industries such asin the manufacture of paint, glue and others. Toluene has lipophilic properties andtoxic effects to some organs such as the central nervous system. At this stage ofbiomolecular, toluene alters the structure of the lipids in cell membranes, resultingin an increased of plasma and tissue levels of MDA. In the Central NervousSystem, toluene can cross the blood brain barrier and cause a disruption in thecerebellum of the brain, thereby increasing the levels of MDA and structuralchanges in the structure of astrocytes’ cells.Methods : To determine the effect of toluene exposure for 14 days at doses belowthe threshold value on the organ brain cerebellum and cerebellar MDAexamination of the brain, as well as causing damage to Astrocytes cells, using fivegroups of male Wistar rats with four types of exposure of 1.6 ml; 3.2 ml; 6.4 ml;12.8 ml; and a control group without exposure.Results : MDA value analysis test brain cerebellum using One Way Anovashowed no significance mean difference (p = 0.133) between the exposed groupand the control group. From the analysis of the number of cells Astrocytes usingOne Way Anova that obtained (p = 0.310) with no difference in outcomes amongexposed groups.Conclusion : There was no significance difference in the group mean exposure toMDA values and the number of cells of the cerebellum Brain Astrocytes exposedto toluene at a dose below the threshold value, Backgrounds : Toluene is a solvent commonly used in various industries such asin the manufacture of paint, glue and others. Toluene has lipophilic properties andtoxic effects to some organs such as the central nervous system. At this stage ofbiomolecular, toluene alters the structure of the lipids in cell membranes, resultingin an increased of plasma and tissue levels of MDA. In the Central NervousSystem, toluene can cross the blood brain barrier and cause a disruption in thecerebellum of the brain, thereby increasing the levels of MDA and structuralchanges in the structure of astrocytes’ cells.Methods : To determine the effect of toluene exposure for 14 days at doses belowthe threshold value on the organ brain cerebellum and cerebellar MDAexamination of the brain, as well as causing damage to Astrocytes cells, using fivegroups of male Wistar rats with four types of exposure of 1.6 ml; 3.2 ml; 6.4 ml;12.8 ml; and a control group without exposure.Results : MDA value analysis test brain cerebellum using One Way Anovashowed no significance mean difference (p = 0.133) between the exposed groupand the control group. From the analysis of the number of cells Astrocytes usingOne Way Anova that obtained (p = 0.310) with no difference in outcomes amongexposed groups.Conclusion : There was no significance difference in the group mean exposure toMDA values and the number of cells of the cerebellum Brain Astrocytes exposedto toluene at a dose below the threshold value] |
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