Pengaruh serotipe c streptococcus mutans terhadap tingkat keasaman plak dan saliva pada penderita resesi gingiva dengan hipersensitif dentin (analisis menggunakan real time PCR) = Effect of serotype c streptococcus mutans for plaque and saliva acidity levels in gingival recession with dentin hypersensitivity (analysis using real time PCR)

Albert, author

Pengaruh serotipe c streptococcus mutans terhadap tingkat keasaman plak dan saliva pada penderita resesi gingiva dengan hipersensitif dentin (analisis menggunakan real time PCR) = Effect of serotype c streptococcus mutans for plaque and saliva acidity levels in gingival recession with dentin hypersensitivity (analysis using real time PCR)

Albert; Sri Lelyati; Lessang, Robert, supervisor; Yulianti Kemal, examiner; Hari Sunarto, examiner (Fakultas Kedokteran Gigi Universitas Indonesia, 2014)

Abstrak

[ABSTRAK

Latar Belakang: Hipersensitif dentin dipengaruhi oleh akumulasi plak pada

permukaan gigi dan penetrasi bakteri pada tubulus dentin. Tujuan: Menganalisis

proporsi serotipe c Streptococcus mutans dan tingkat keasaman di dalam plak

dan saliva penderita resesi gingiva yang hipersensitif dentin dengan penderita

resesi gingiva yang non hipersensitif. Metode: Tiga puluh enam sampel plak dan

saliva dibagi dalam dua kelompok yaitu kelompok hipersensitif dan non

hipersensitif. Dilakukan ekstrak DNA sampel, pengukuran pH sampel dan

evaluasi amplifikasi serotipe c Streptococcus mutans dengan alat Real Time

PCR. Hasil: Proporsi serotipe c Streptococcus mutans di dalam plak dan

saliva tidak berbeda pada penderita resesi gingiva dengan hipersensitif

dentin maupun non hipersensitif, Kesimpulan: Proporsi serotipe c

Streptococcus mutans plak dan saliva tidak mempengaruhi hipersensitif

dentin.

ABSTRACT

Background: dentin hypersensitivity is affected by the accumulation of plaque

on the tooth surface and penetration of bacteria in the dentinal tubules.

Objective: To analyze the proportion of serotype c Streptococcus mutans and the

level of acidity in plaque and saliva of patients with hypersensitive dentin and

non hypersensitive. Methods: Thirty-six plaque and saliva samples were divided

into two groups: the hypersensitive and non-hypersensitive. Extract the sample

DNA, measure the acidity levels and evaluate serotype c Streptococcus mutans

amplification with Real Time PCR. Results: The proportion of serotype c

Streptococcus mutans in plaque and saliva is not significantly different in the

patients with gingival recession both hypersensitive and non-hypersensitive,

Conclusions: The proportion of serotype c Streptococcus mutans in plaque and

saliva are equally well both in hypersensitive and non hypersensitive cases.;Background: dentin hypersensitivity is affected by the accumulation of plaque