[ABSTRAK
Doksorubisin merupakan obat pilihan utama dalam terapi kanker, tetapi memilikiindeks terapi rendah. Sehubungan dengan alasan tersebut maka pada penelitian inidilakukan pembuatan dan karakterisasi nanopartikel emas (AuNP) - gom arabterfungsionalisasi doksorubisin untuk meningkatkan indeks terapinya. AuNPdibuat dengan mereduksi HAuCl4 dengan natrium sitrat kemudian ditambahkangom arab sebagai penstabil (GA-AuNP) dan setelah itu difungsionalisasikandengan doksorubisin (Dox-GA-AuNP). Dox-GA-AuNP dikarakterisasi denganspektrofotometri UV-Vis, spektrofotometri infra merah, dynamic light scatteringdan transmission electron microscopy. Doksorubisin memiliki spektrum serapanUv-Vis maksimal pada panjang gelombang 479 nm, sedangkan Dox-GA-AuNPmemiliki spektrum serapan Uv-Vis maksimal pada panjang gelombang 485 nm.Spektrum IR Dox-GA-AuNP menunjukan adanya pita serapan ikatan keton danamin yang berbeda dengan pita serapan ikatan keton dan amin pada doksorubisinbebas. Ukuran partikel Dox-GA-AuNP adalah 113,6 nm dengan karakteristikmonodispersi (PDI 0,423), dan memiliki morpologi berbentuk sferis. Pengujiansitotoksik dilakukan terhadap doksorubisin bebas dan Dox-GA-AuNP. Hasil yangdiperoleh menunjukkan bahwa pengujian sitotoksisitas Dox-GA-AuNP pada linisel MCF-7 memberikan IC50 0,28 μg/mL sedangkan doksorubisin bebas memilikiIC50 1,305 μg/mL. Doksorubisin yang telah difungsionalisasikan dengan GAAuNPdapat mengurangi ikatan protein dengan serum albumin manusia dari 62,51± 2,21 % (Dox Bebas) menjadi 22,91 ± 10,9 % (Dox-GA-AuNP). Denganmenurunnya ikatan protein dan meningkatnya efek sitotoksisitas pada Dox-GAAuNPdibandingkan dengan doksorubisin bebas dapat disimpulkan bahwa Dox-GA-AuNP dapat meningkatkan indeks terapi doksorubisin.ABSTRACT
Doxorubicin is a drug of choice for cancer therapy, but it has low index therapy.For that reason the reseach had been done to make and characterize goldnanoparticle - acacia gum functionalized doxorubicin to increase the therapyindex. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrateand gum arabic was added as a steric stabilizer, and then being functionalized bydoxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Visspectrophotometry, infrared spectrophotometry, dynamic light scattering andelectron microscopy transmission. The UV-Vis spectrometry showed thatdoxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNPis 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketoneand amine bonds absorption band which is different from absorption band ofdoxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with PolyDispersion Index of 0.423, and morphological shape is spheric. The cytotoxicassay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. Theresults showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing ofprotein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared todoxorubicin conclude that Dox-GA-AuNP can increase the therapy index ofdoxorubicin.;Doxorubicin is a drug of choice for cancer therapy, but it has low index therapy.For that reason the reseach had been done to make and characterize goldnanoparticle - acacia gum functionalized doxorubicin to increase the therapyindex. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrateand gum arabic was added as a steric stabilizer, and then being functionalized bydoxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Visspectrophotometry, infrared spectrophotometry, dynamic light scattering andelectron microscopy transmission. The UV-Vis spectrometry showed thatdoxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNPis 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketoneand amine bonds absorption band which is different from absorption band ofdoxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with PolyDispersion Index of 0.423, and morphological shape is spheric. The cytotoxicassay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. Theresults showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing ofprotein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared todoxorubicin conclude that Dox-GA-AuNP can increase the therapy index ofdoxorubicin., Doxorubicin is a drug of choice for cancer therapy, but it has low index therapy.For that reason the reseach had been done to make and characterize goldnanoparticle - acacia gum functionalized doxorubicin to increase the therapyindex. Gold nanoparticles was prepared by reducing HAuCl4 with sodium citrateand gum arabic was added as a steric stabilizer, and then being functionalized bydoxorubicin (Dox-GA-AuNP). Dox-GA-AuNP was characterized by UV - Visspectrophotometry, infrared spectrophotometry, dynamic light scattering andelectron microscopy transmission. The UV-Vis spectrometry showed thatdoxorubicin has a maximum spectrum absorbtion of 479 nm while Dox-GAAuNPis 485 nm, FTIR spectrofotometcy showed that Dox-GA-AuNP has ketoneand amine bonds absorption band which is different from absorption band ofdoxorubicin. The particle size of Dox-GA-AuNP is 113.6 nm with PolyDispersion Index of 0.423, and morphological shape is spheric. The cytotoxicassay was conducted on MCF-7 cell line for Dox-GA-AuNP and doxorubicin. Theresults showed that Dox-GA-AuNP provides IC50 of 0.28 mg / mL while the IC50of doxorubicin is 1.305 mg / mL. Protein bond of Dox-GA-AuNP is 22.91 ± 10.9% while protein bond of doxorubicin is 62.51 ± 2.21 %. The decreasing ofprotein bond and increasing of cytotoxicity effect of Dox-GA-AuNP compared todoxorubicin conclude that Dox-GA-AuNP can increase the therapy index ofdoxorubicin.] |
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