ABSTRAK
Latar belakang: Penuaan paru ditandai dengan perubahan struktur dan fisiologi paru. Secara struktural, terjadi perubahan ketebalan septum interalveolar dan komponen di dalamnya, salah satunya adalah serat kolagen interstisial, sehingga dapat memengaruhi fungsi paru sebagai organ respirasi. Tujuan dari penelitian ini adalah mengetahui korelasi antara jumlah serat kolagen interstitial paru dengan ketebalan septum interalveolar pada penuaan tikus Sprague-Dawley.Metode: Desain penelitian yang digunakan adalah cross sectional analytic correlative. Penelitian ini menggunakan 24 ekor tikus Sprague-Dawley sebagai subjek penelitian. Subjek penelitian terdiri atas empat kelompok usia, yaitu 2 hari, 16 hari, 3-4 bulan, dan >12 bulan yang ditentukan dengan m± 0.043 μm, dan 0,512 ± 0.020 μm. Uji korelasi non parametrik Spearman (p =0,03) antara jumlah serat kolagen interstisial dengan ketebalan septuminteralveolar menunjukkan nilai koefisien korelasi (r = 0,213).Kesimpulan: Pada penelitian ini disimpulkan bahwa terdapat korelasi yang lemahantara jumlah serat kolagen interstisial paru dengan ketebalan septuminteralveolar pada penuaan tikus Sprague-Dawley. Dengan demikian, dapatdipikirkan bahwa serat kolagen interstisial dapat mempengaruhi ketebalan septuminteralveolar paru tikus yang menua, meskipun bukan satu-satunya faktor yangmempengaruhi.ABSTRACT
Background: Lung aging is characterized by structure and physiologic changesof the lung. Structurally, the interalveolar septum thickness and all of itscomponents including collagen fiber are change, so that affect the lung function asa respiratory organ. This study is aimed to determine the correlation between theamount of pulmonary interstitial collagen fiber and interalveolar septum thicknesson Sprague-Dawley rat aging.Method: The design of this research is cross sectional analytic correlative. Thedata was taken from the lung tissue preparations of 24 rats in 4 groups based onage, 2 days, 16 days, 3-4 months, and >12 months using single blindrandomization technique. The methods of preparation making are based on theliteratures. Data that was assessed were the histology of pulmonary interstitialcollagen fiber and interalveolar septum. Then, they were analized using SPSS20.0.Result: Sequentially, the modes of interstitial collagen fiber are 1, 2, 2, and 3;while the interalveolar septum thickness means are 0,436 ± 0.059 μm, 0,399 ±0.022 μm, 0,474 ± 0.043 μm, and 0,512 ± 0.020 μm. By using non parametricSpearman correlation test (p = 0.03), it was obtained the correlation coefficient (r= 0.213) between the amount of pulmonary interstitial collagen fiber with theinteralveolar septum thickness.Conclusion: There is a weak correlation between the amount of pulmonaryinterstitial collagen fiber with the interalveolar septum thickness of Sprague-Dawley rat aging. Thus, it can be thought that pulmonary interstitial collagen fibermay affects interalveolar septum thickness of rat aging, although it’s not as theonly one factor. |
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