ABSTRAK
Telah dilakukan penelitian yang bertujuan memperoleh identitas dua isolat bakteritermofilik dari geiser. Isolat LC2-23 diperoleh dari serasah pada geiser diCisolok, Jawa Barat, Indonesia, dan isolat RKB-2 diperoleh dari serasah padageiser di Onikobe, Miyagi, Jepang.!!Identifikasi dilakukan berdasarkan gabungandata fenotipik dan genotipik. Berdasarkan karakterisasi fenotipik, isolat LC2-23memiliki sel berbentuk batang; menghasilkan endospora; motil; gram positif;bersifat aerob dan fakultatif aerob; mampu tumbuh pada suhu 60 oC, sedangkansuhu optimum pertumbuhan 50 oC. Berdasarkan karakterisasi genotipik, data fullsequence gen 16S rRNA isolat LC2-23 memiliki homologi 99,1% terhadapBrevibacillus agri. Berdasarkan data fenotipik dan genotipik, isolat LC2-23diidentifikasi sebagai Brevibacillus agri (Family Paenibacillaceae, OrderBacillales, Class Bacilli, Phylum Firmicutes). Berdasarkan karakterisasifenotipik, isolat RKB-2 membentuk miselium vegetatif dan aerial yangbercabang; menghasilkan spora aerial; gram positif; bersifat aerob; mamputumbuh pada suhu 60 oC, sedangkan suhu optimum pertumbuhan 50 oC.Berdasarkan karakterisasi genotipik, data full sequence gen 16S rRNA isolatRKB-2 memiliki homologi yang rendah, yaitu 98,4% terhadap spesiesterdekatnya, Thermosporothrix hazakensis (Family Thermosporotrichaceae,Order Ktedonobacteriales, Class Ktedonobacteria, Phylum Chloroflexi). Hasilanalisis filogenetik menunjukkan posisi isolat RKB-2 terpisah dari T. hazakensis.Data kemotaksonomi (komposisi asam lemak) dan hasil analisis proteomikmenggunakan MALDI-TOF MS mendukung perbedaan antara isolat RKB-2 danT. hazakensis. Berdasarkan perbedaan tersebut isolat RKB-2 diidentifikasisebagai spesies baru dari Thermosporothrix. Untuk pengajuan nama spesies barudiperlukan data hibridisasi DNA-DNA antara isolat RKB-2 dengan T. hazakensis.
ABSTRACT
This research was aimed to identify two bacterial isolates obtained from geysers.Strain LC2-23 was isolated from litters on a geyser in Cisolok, West Java,Indonesia, and isolate RKB-2 was obtained from litters on a geyser in Onikobe,Miyagi Prefecture, Japan. Identification of bacteria was based on integrated dataof phenotypic and genotypic characterizations. Based on phenotypiccharacterizations of isolate LC2-23: it has a rod (bacilli)-shaped cells, formsendospores; gram positive; motile; aerobic, and able to grow up to a temperatureof 60 oC. Based on genotypic characterizations of isolate LC2-23: the fullsequence of genes 16S rRNA shows 99.1% sequence homology to Brevibacillusagri. Based on phenotypic and genotypic data, isolate LC2-23 can be identifiedas Brevibacillus agri (Family Paenibacillaceae, Order Bacillales, Class Bacilli,Phylum Firmicutes). Based on phenotypic characterizations of isolate RKB-2:vegetative and branching aerial mycelia forms, gram positive, aerobic, and able togrow up to a temperature of 60 oC. Based on genotypic characterizations ofisolate RKB-2: the full sequence of 16S rRNA gene of isolate RKB-2 showed lowhomology (98.4%) to Thermosporothrix hazakensis (FamilyThermosporotrichaceae, Order Ktedonobacteriales, Class Ktedonobacteria,Phylum Chloroflexi). Phylogenetic analysis showed the isolate RKB-2 wasdistinct from cluster of Thermosporothrix hazakensis and Ktedonobacteriabacterium. The genotypic and phylogenetic data, plus chemotaxonomic andproteomic analysis using MALDI-TOF MS, suggest that isolate RKB-2 representnovel species of the genus Thermosporothrix. The DNA-DNA hibridization datais needed for proposal of new species. |
S60542-Abinubli Tariswafi Mawarid.pdf :: Unduh