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Deteksi high risk human papilloma virus pada jaringan adenoma dan adenokarsinoma kolorektal di FKUI RSCM = Detection of high risk human papilloma virus on colorectal adenoma and adenocarcinoma tissue in FKUI RSCM / Wiwiek Ernajanti

Wiwiek Ernajanti; Diah Rini Handjari, supervisor; Kusmardi, supervisor; Siregar, Nuryati Chairani, examiner; Ening Krisnuhoni, examiner ([Publisher not identified] , 2015)

 Abstrak

[ABSTRAK
Latar Belakang: Kanker kolorektal merupakan tumor ganas ketiga di dunia.
Sembilan puluh lima persen kanker kolorektal merupakan adenokarsinoma yang
berasal dari lesi prekursor adenoma. Dilaporkan 15%-20% kanker terkait dengan
infeksi virus. Virus yang diduga berhubungan dengan kanker kolorektal adalah
human papilloma virus (HPV) dan tipe tersering adalah 16 dan 18. Hubungan
antara HPV dan kanker kolorektal masih menjadi perdebatan. Penelitian ini
bertujuan untuk mengetahui perbedaan prevalensi infeksi HPV pada adenoma dan
adenokarsinoma kolorektal di Departemen Patologi Anatomik FKUI/RSCM
Jakarta dengan menggunakan teknik polymerase chain reaction (PCR). Bahan
dan Metode: Pemeriksaaan DNA HPV pada 33 kasus adenoma dan 33 kasus
adenokarsinoma kolorektal dengan teknik nested PCR MY/GP dan elektroforesis.
Pada kasus dengan hasil HPV positif, dilanjutkan PCR menggunakan primer
spesifik HPV 16 dan HPV 18. Subjek penelitian berasal dari Departemen Patologi
Anatomik FKUI/RSCM. Hasil: Satu dari 33 kasus (3,0%) adenoma dan 3 dari 33
kasus (9,1%) adenokarsinoma positif infeksi HPV. Satu kasus adenoma positif
HPV bukan merupakan tipe 16 dan 18. Satu kasus adenokarsinoma dengan
positif, HPV merupakan tipe 16, 2 kasus merupakan gabungan tipe 16 dan 18.
Kesimpulan: Prevalensi infeksi HPV pada adenokarsinoma lebih tinggi
dibandingkan adenoma kolorektal. Tipe HPV pada kasus adenokarsinoma
kolorektal merupakan tipe 16 dan 18.

ABSTRACT
Background : Colorectal cancer is the third malignant tumor in the world.
Ninety-five percent of colorectal cancers are adenocarcinomas derived from
precursor lesions adenoma. There are 15% -20% of cancers associated with viral
infections. Virus are suspected associated with colorectal cancer is the human
papilloma virus (HPV) and the most common types are 16 and 18. The
relationship between HPV and colorectal cancer is still being debated. This study
purpose to determine the prevalence differences of HPV infection in colorectal
adenomas and adenocarcinomas in the Department of Anatomic Pathology,
FKUI/RSCM Jakarta by using the polymerase chain reaction (PCR). Materials
and Methods : HPV DNA examination on 33 cases of adenoma and 33 cases of
colorectal adenocarcinoma by nested MY/GP PCR technique and electrophoresis.
In the cases with positive HPV results, continue by specific primers HPV 16 and
HPV 18 PCR. The subject of the study came from the Department of Anatomic
Pathology, FKUI/RSCM. Result : One (3.0%) adenomas and 3 (9.1%)
adenocarcinoma from 33 cases adenoma and adenocarcinoma are HPV positive.
One case of HPV positive adenomas are not types 16 and 18. HPV positive
adenocarcinoma, 1 case was type 16, two cases are combination of types 16 and
18. Conclusion : The HPV prevalence in adenocarcinoma was higher than
colorectal adenoma. HPV types on positive colorectal adenocarcinoma cases are
types 16 and 18., Background : Colorectal cancer is the third malignant tumor in the world.
Ninety-five percent of colorectal cancers are adenocarcinomas derived from
precursor lesions adenoma. There are 15% -20% of cancers associated with viral
infections. Virus are suspected associated with colorectal cancer is the human
papilloma virus (HPV) and the most common types are 16 and 18. The
relationship between HPV and colorectal cancer is still being debated. This study
purpose to determine the prevalence differences of HPV infection in colorectal
adenomas and adenocarcinomas in the Department of Anatomic Pathology,
FKUI/RSCM Jakarta by using the polymerase chain reaction (PCR). Materials
and Methods : HPV DNA examination on 33 cases of adenoma and 33 cases of
colorectal adenocarcinoma by nested MY/GP PCR technique and electrophoresis.
In the cases with positive HPV results, continue by specific primers HPV 16 and
HPV 18 PCR. The subject of the study came from the Department of Anatomic
Pathology, FKUI/RSCM. Result : One (3.0%) adenomas and 3 (9.1%)
adenocarcinoma from 33 cases adenoma and adenocarcinoma are HPV positive.
One case of HPV positive adenomas are not types 16 and 18. HPV positive
adenocarcinoma, 1 case was type 16, two cases are combination of types 16 and
18. Conclusion : The HPV prevalence in adenocarcinoma was higher than
colorectal adenoma. HPV types on positive colorectal adenocarcinoma cases are
types 16 and 18.]

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No. Panggil : T-Pdf
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Entri tambahan-Nama badan :
Subjek :
Penerbitan : [Place of publication not identified]: [Publisher not identified], 2015
Program Studi :
Bahasa : ind
Sumber Pengatalogan : LibUI ind rda
Tipe Konten : text
Tipe Media : computer
Tipe Carrier : online resource
Deskripsi Fisik : xiii, 75 pages : illustration ; 28 cm + appendix
Naskah Ringkas :
Lembaga Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 3
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T-Pdf 15-17-618019831 TERSEDIA
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