[ABSTRAK
Pasak bumi (PB) (Eurycoma longifolia Jack), adalah tanaman herbal Indonesia yangdigunakan sebagai antimalaria. Penelitian terdahulu meliputi efek anti ageing dan antiinflamasi, namun belum pernah diteliti tentang efek terhadap aktivitas enzimantioksidan pada penggunaan ekstrak akar PB. Penelitian ini bertujuan untukmengetahui apakah pengaruh ekstrak akar PB sebagai antimalaria dapat menurunkanaktivitas spesifik antioksidan enzimatik. Penelitian ini menggunakan mencit yangdiinfeksi Plasmodium berghei, diterapi dengan ekstrak akar PB, klorokuin 10 mg/kgBB (kontrol positif, KP), kontrol negatif (akuades, KN), kontrol normal (K0), PB 30(TI), 60 (TII) dan 90 mg/kg BB (TIII). Parameter yang diukur adalah inhibisiparasitemia, kadar karbonil, aktivitas spesifik SOD, katalase (CAT). Inhibisiparasitemia hari ke 7 dari KP, TI, TII dan TIII adalah 69,81%, 39,37%, 41,72% dan12,92%. Aktivitas spesifik enzim SOD dan CAT plasma tidak ada perbedaanbermakna. Aktivitas spesifik SOD hati menunjukan perbedaan bermakna antara K0-KN (p=0,000), K0-KP (p= 0,025), KN-TI (p=0,001), KP-TI (p=0,042), KN-TII(p=0,002), KN-TIII (0,005). Aktivitas spesifik CAT hati menunjukkan perbedaanbermakna antara KP-TI (p=0,009), KP-TII (p=0,009), KP-TIII (p=0,014), KP-K0(p=0,009), TI-TIII (p=0,014), KN-TI (p=0,009), KN-TII (p=0,047), K0-KN(p=0,047). Kadar karbonil plasma dan hati tidak menunjukkan perbedaan bermaknaantar kelompok. Korelasi positif bermakna (r=0,690, p=0,000) terjadi antara aktivitasspesifik SOD dan CAT hati. Korelasi negatif bermakna terjadi antara aktivitasspesifik SOD, CAT hati dan parasitemia (r= -0,637, p=0,000) (r=-0,557, p=0,002).Kesimpulan: Potensi PB sebagai antimalaria diragukan karena herbal ini jugamemiliki efek antioksidan yang menguntungkan bagi parasit.
ABSTRACT
Pasak bumi (PB)(Eurycoma longifolia Jack), is an Indonesian herb used asantimalarial. Previous studies had been done on its anti-ageing and anti-inflammationproperties, but its effect on antioxidant enzyme had not been researched. This studyaim to investigate the antimalarial influence of PB extract on the reduction of specificantioxidant activity of the SOD and CAT enzyme. We used mice infected byPlasmodium berghei treated with: PB 30, 60, and 90 mg/kg BW as (TI, TII, andTIII), positive control (chloroquine 10 mg/kg BW) (KP), negative control (aquadest)(KN), normal mice control (K0). The parameters were: growth inhibition, carbonylconcentration, specific activity of SOD and CAT. Growth inhibition in 7 day groupsof KP, TI, TII, and TIII were 69,81%, 39,37%, 41,72%, and 12,92%. Specific activityof plasma SOD and CAT were insignificant between groups. Liver SOD specificactivity showed significant different between K0-KN (p=0,000), K0-KP (p= 0,025),KN-TI (p=0,001), KP-TI (p=0,042), KN-TII (p=0,002), KN-TIII (0,005). Specificactivity of liver CAT showed significant different between KP-TI (p=0,009), KP-TII(p=0,009), KP-TIII (p=0,014), KP-K0 (p=0,009), TI-TIII (p=0,014), KN-TI(p=0,009), KN-TII (p=0,047), K0-KN (p=0,047). Carbonyl concentrations showinsignificant between groups in plasma and liver. Positive correlation (r=0,690,p=0,000) showed between liver SOD and CAT specific activity, negative correlationshowed between liver SOD (r= -0,637, p=0,000), CAT (r= -0,557, p=0,002) specificactivity and paracytemia. Therefore, The potential use of PB as an antimalarial was ofdoubtful effectiveness due to its antioxidant effect which could be beneficial to theparasite, Pasak bumi (PB)(Eurycoma longifolia Jack), is an Indonesian herb used asantimalarial. Previous studies had been done on its anti-ageing and anti-inflammationproperties, but its effect on antioxidant enzyme had not been researched. This studyaim to investigate the antimalarial influence of PB extract on the reduction of specificantioxidant activity of the SOD and CAT enzyme. We used mice infected byPlasmodium berghei treated with: PB 30, 60, and 90 mg/kg BW as (TI, TII, andTIII), positive control (chloroquine 10 mg/kg BW) (KP), negative control (aquadest)(KN), normal mice control (K0). The parameters were: growth inhibition, carbonylconcentration, specific activity of SOD and CAT. Growth inhibition in 7 day groupsof KP, TI, TII, and TIII were 69,81%, 39,37%, 41,72%, and 12,92%. Specific activityof plasma SOD and CAT were insignificant between groups. Liver SOD specificactivity showed significant different between K0-KN (p=0,000), K0-KP (p= 0,025),KN-TI (p=0,001), KP-TI (p=0,042), KN-TII (p=0,002), KN-TIII (0,005). Specificactivity of liver CAT showed significant different between KP-TI (p=0,009), KP-TII(p=0,009), KP-TIII (p=0,014), KP-K0 (p=0,009), TI-TIII (p=0,014), KN-TI(p=0,009), KN-TII (p=0,047), K0-KN (p=0,047). Carbonyl concentrations showinsignificant between groups in plasma and liver. Positive correlation (r=0,690,p=0,000) showed between liver SOD and CAT specific activity, negative correlationshowed between liver SOD (r= -0,637, p=0,000), CAT (r= -0,557, p=0,002) specificactivity and paracytemia. Therefore, The potential use of PB as an antimalarial was ofdoubtful effectiveness due to its antioxidant effect which could be beneficial to theparasite] |
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