[ABSTRAK
Latar belakang: Pematangan sperma di epididimis terjadi melalui interaksiantara protein yang disekresikan oleh epitel dengan spermatozoa. Proses tersebutdiregulasi oleh androgen dan lingkungan spesifik di region epididimis. Androgendependentgene yang hanya terekspresi di region tertentu namun tidak terekspresidi region lain menimbulkan dugaan peran androgent reseptor (AR) koregulator.Gelsolin (Gsn) adalah AR koregulator ditemukan predominan di epididimisHolstein, namun perannya masih belum diketahui. Penelitian ini bertujuan untukmengkarakterisasi Gsn pada epididimis mencit.Metode: In silico untuk memprediksi struktur gen dan domain fungional.Quantitative Real Time RT-PCR untuk menganalisa sebaran jaringan,ketergantungan terhadap faktor endokrin dan faktor testikular, dan regulasipostnatal.Hasil: Gsn merupakan protein yang mengandung signal peptide. Ekspresi Gsntidak spesifik di epididimis. Gsn dipengaruhi oleh androgen dan faktor testikular.Pasca gonadektomi, ekspresi Gsn menurun setelah 3 hari dan injeksi T eksogenmeningkatkan ekspresi Gsn. Hasil ini diperkuat dengan pemberian flutamide yangmenurunkan ekspresi Gsn. Ekspresi Gsn pada perkembangan individu konstanpostnatal 5 hari.Kesimpulan: Gsn adalah protein yang disekresikan oleh epitel epididimis,diregulasi oleh androgen dan faktor testikular. Ekspresi Gsn yang tidak spesifikpada region tertentu di epididimis, diperlukan penelitian lanjut untuk mengetahuiperan Gsn dalam menentukan ekspresi gen-gen yang terlibat dalam pematangansperma.
ABSTRACT
Background: Sperm maturation in epididymis occurs through interactionbetween proteins secreted by epithels with spermatozoa. The process is regulatedby androgen and specific environment in epididimal region. The androgendependentgene that is only expressed in a particular region, but not expressed inother regions led to allegations of androgen receptor (AR) coregulator action.Gelsolin (Gsn) is AR coregulator found predominant in epididimal Holstein, butits role still unknown. The aim is to characterize Gsn in mouse epididymis.Methods: In silico analyses to predict gene strucure and functional domain.Quantitative Real Time RT-PCR to analyse tissue distribution, androgendependent, testicular factor and postnatal regulation.Results: Gsn is protein that contains signal peptide. Gsn is not spesific expressedin epididymis. It is regulated by androgen and testicular factor. Post gonadectomy,Gsn expression decrease in 3 days while injected by T exogen increasing Gsnexpression. This expression confirmed by flutamide that decreasing Gsnexpression. Gsn expression was constant at day 5 in postnatal development.Conclusions: Gsn is protein that secreted by epididymal epitels and regulated byandrogen and testicular factor. Gsn expression was not spesific in epididymalregion. It is needed to do future research to know the role of Gsn in determininggenes expression that related to sperm maturation, Background: Sperm maturation in epididymis occurs through interactionbetween proteins secreted by epithels with spermatozoa. The process is regulatedby androgen and specific environment in epididimal region. The androgendependentgene that is only expressed in a particular region, but not expressed inother regions led to allegations of androgen receptor (AR) coregulator action.Gelsolin (Gsn) is AR coregulator found predominant in epididimal Holstein, butits role still unknown. The aim is to characterize Gsn in mouse epididymis.Methods: In silico analyses to predict gene strucure and functional domain.Quantitative Real Time RT-PCR to analyse tissue distribution, androgendependent, testicular factor and postnatal regulation.Results: Gsn is protein that contains signal peptide. Gsn is not spesific expressedin epididymis. It is regulated by androgen and testicular factor. Post gonadectomy,Gsn expression decrease in 3 days while injected by T exogen increasing Gsnexpression. This expression confirmed by flutamide that decreasing Gsnexpression. Gsn expression was constant at day 5 in postnatal development.Conclusions: Gsn is protein that secreted by epididymal epitels and regulated byandrogen and testicular factor. Gsn expression was not spesific in epididymalregion. It is needed to do future research to know the role of Gsn in determininggenes expression that related to sperm maturation] |
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