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[Penicillium marneffei merupakan fungi patogen yang ditemukan di AsiaTenggara, khususnya Thailand. Penisiliosis dapat menyebabkan mikosis sistemiksehingga membahayakan nyawa penderita immunocompromised, khususnyapenderita HIV/AIDS. Antifungi seperti Fluconazole dan Ketoconazole, digunakanuntuk mengatasi infeksi P. marneffei. Akan tetapi, penggunaan antifungi secarajangka panjang dapat memicu kemungkinan munculnya mutan resisten P.marneffei. Resistensi pada fungi dapat dipengaruhi beberapa faktor, salah satunya,overekspresi transporter pengeluaran obat (drug efflux transporter). Mekanismepompa pengeluaran obat diatur oleh berbagai transporter. Transporter yang palingumum diketahui ialah transporter ABC (ATP-binding-cassette) dan MFS (MajorFacilitator Superfamily). Transporter ABC multidrug (MDR) pada P. marneffeitelah dipelajari dengan baik, sedangkan transporter MFS MDR pada fungitersebut, belum mendapatkan perhatian yang sama. Penelitian ini fokus pada satutransporter MFS MDR P. marneffei, yakni PMAA 067100, yang diekspresikanpada Saccharomyces cerevisiae ADΔ; sistem ekspresi yang sangat rentan terhadapberbagai macam antifungi. Pengamatan melalui mikroskop konfokal dan uji DiskDiffusion menunjukkan bahwa transporter PMAA 067100 terlokalisasi padamembran sel S. cerevisiae ADΔ dan resisten terhadap Fluconazole danTerbinafine.;Penicillium marneffei has been known as a pathogenic fungi which isfound in Southeast Asia, especially Thailand. The infection by this fungirecognized as Penicilliosis, that caused systemic mycosis, might be lethal inimmunocompromised patient, specifically HIV/AIDS patient. Antifungal such asFluconazole and Ketoconazole, had been used against P. marneffei infection.However, the long-term-use of antifungal might cause an emerging resistant strainof P. marneffei. The resistance phenomenon in fungi is caused by several factors,one of it is the overexpression of drug efflux transporter. Mechanism of this effluxpump is regulated by some of transporters such as ABC (ATP-binding-cassette)and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)transporter of P. marneffei has been studied well, yet the underrated MFS MDRtransporter of the same fungi has not received the same attention. This study focuson one of P. marneffei MFS MDR transporter, known as PMAA 067100, whichwas expressed in Saccharomyces cerevisiae ADΔ; an expression system which isvery susceptible to many kind of antifungal. Observation through confocalmicroscope and Disk Diffusion test showed that PMAA 067100 transporter waslocalized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazoleand Terbinafine., Penicillium marneffei has been known as a pathogenic fungi which isfound in Southeast Asia, especially Thailand. The infection by this fungirecognized as Penicilliosis, that caused systemic mycosis, might be lethal inimmunocompromised patient, specifically HIV/AIDS patient. Antifungal such asFluconazole and Ketoconazole, had been used against P. marneffei infection.However, the long-term-use of antifungal might cause an emerging resistant strainof P. marneffei. The resistance phenomenon in fungi is caused by several factors,one of it is the overexpression of drug efflux transporter. Mechanism of this effluxpump is regulated by some of transporters such as ABC (ATP-binding-cassette)and MFS (Major Facilitator Superfamily) transporter. The ABC multidrug (MDR)transporter of P. marneffei has been studied well, yet the underrated MFS MDRtransporter of the same fungi has not received the same attention. This study focuson one of P. marneffei MFS MDR transporter, known as PMAA 067100, whichwas expressed in Saccharomyces cerevisiae ADΔ; an expression system which isvery susceptible to many kind of antifungal. Observation through confocalmicroscope and Disk Diffusion test showed that PMAA 067100 transporter waslocalized in S. cerevisiae ADΔ cell membrane and resistant against Fluconazoleand Terbinafine.] |
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