|
[Saat ini tengah banyak studi yang dilakukan untuk menemukan antivirus dengue(DENV) dari ekstrak bebagai tumbuhan sebagai alternatif pengobatan terhadapinfeksi DENV. Calophyllum flavoramulum (C. flavoramulum) merupakantanaman yang banyak hidup di daerah Asia Tenggara ini memiliki kandungan dariturunan xanthone dan flavonoid yang memiliki potensi sebagai antivirus. Padastudi ini dilakukan evaluasi efek inhibisi dari fraksi etanol ekstrak daun C.flavoramulum terhadap DENV-2 strain New Guinea C (NGC). Efek inhibisi(IC50) dievaluasi dengan menggunakan focus assay. Sedangkan efek toksisitas(CC50) terhadap sel dievaluasi pada sel Huh7it-1 menggunakan metode MTTassay. Hasil dari studi ini menunjukan CC50 = 473,50ug/ml, IC50 = 41,74ug/ml,dan SI=11,33. Hasil tersebut menunjukan bahwa C. flavoramulum tidak toksikterhadap sel hidup dan memiliki nilai SI yang cukup tinggi sehingga efektif untukdigunakan sebagai antivirus dengue. Akan tetapi, perlu diteliti lebih lanjutmengenai kandungan aktif dalam C. flavoramulum yang memiliki aktivitas untukmenghambat replikasi DENV.;In recent years, several studies have been conducted to find dengue (DENV)antiviral from natural extract as an alternative management for dengue infection.Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,contains derivates of flavonoid and xanthone where both have been known aspotential dengue antivirals. This study evaluated inhibitory potential of ethanolextract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) toHuh7it-1 cell also evaluated through MTT assay. The result of this study shownIC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extractof C. flavoramulum can be used selectively as a dengue antiviral, besides it is nottoxic for living cells. More studies are needed to find its active ingredients whichspecifically have the ability to inhibit DENV replication.;In recent years, several studies have been conducted to find dengue (DENV)antiviral from natural extract as an alternative management for dengue infection.Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,contains derivates of flavonoid and xanthone where both have been known aspotential dengue antivirals. This study evaluated inhibitory potential of ethanolextract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) toHuh7it-1 cell also evaluated through MTT assay. The result of this study shownIC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extractof C. flavoramulum can be used selectively as a dengue antiviral, besides it is nottoxic for living cells. More studies are needed to find its active ingredients whichspecifically have the ability to inhibit DENV replication., In recent years, several studies have been conducted to find dengue (DENV)antiviral from natural extract as an alternative management for dengue infection.Calophyllum flavoramulum(C. flavoramulum), one of South East Asia plants,contains derivates of flavonoid and xanthone where both have been known aspotential dengue antivirals. This study evaluated inhibitory potential of ethanolextract from C. flavoramulum leaf to DENV-2 strain New Guinea C (NGC)through focus assay. Along with inhibitory effect (IC50), toxicity effect (CC50) toHuh7it-1 cell also evaluated through MTT assay. The result of this study shownIC50 = 41.74 μg/mL, CC50 = 473.50 μg/mL, and SI=11.33. In conclusion, extractof C. flavoramulum can be used selectively as a dengue antiviral, besides it is nottoxic for living cells. More studies are needed to find its active ingredients whichspecifically have the ability to inhibit DENV replication.] |
Haryo Satrio Muhammad.pdf :: Unduh