ABSTRAK
Pendahuluan. Osteoarthritis (OA) adalah sebuah penyakit sendi degeneratif yangmenyebabkan disabilitas dengan prevalensi yang terus meningkat. Hormonpertumbuhan memiliki efek regenerasi tulang rawan secara langsung melaluistimulasi sel kondroblas dan proses morphoangiogenesis juga melalui faktorpertumbuhan secara sistemik. Penelitian ini bertujuan untuk mengetahui manfaatsuntikan sendi dengan hormon pertumbuhan pada kasus Osteoarthritis. Metode Penelitian. Penelitian dilakukan di Rumah Sakit Hewan InstitutPertanian Bogor pada bulan Mei hingga September 2015. Desain penelitian adalahrandomized post test only control group. Sejumlah 21 ekor kelinci Selandia Baruputih, berat 1.9-2.6kg, usia 7-8 bulan. Kelinci dibagi secara acak menjadikelompok kontrol (NaCl 0.9%), suntikan hormon pertumbuhan (4iu), dan suntikanasam hyaluronat (6mg) . Dengan metode acak tersamar dilakukan suntikankolagenase tipe II C. Histolyticum pada hari 1 dan ke 4 pada lutut kiri, kemudiantindakan penyuntikan dilakukan sebanyak tiga kali dengan selang waktu 1minggu. Evaluasi dengan periode kepincangan, skoring makroskopis, histologisdilakukan pada minggu ke-8 pasca penyuntikan pertama. Temuan Penelitian. Berdasarkan hasil penelitian ditemukan periode kepincanganpada grup yang diberikan hormon pertumbuhan lebih singkat, dan bermaknasecara statistik dibandingkan dengan grup kontrol (p<0.001), grup asamhyaluronat (p<0.03), dan grup hormon pertumbuhan (p<0.001). Evaluasi skormakroskopik dengan skor yoshimi menunjukan bahwa kelompok hormonpertumbuhan memiliki kerusakan tulang rawan yang lebih ringan jikadibandingkan dengan grup kontrol (p=0.001) dan grup asam hyaluronat (p=0.04).Skoring histopatologis menggunakan skor modifikasi Mankin menunjukan padakelompok dengan hormon pertumbuhan memiliki angka terendah dibandingkangrup lainnya (p=0.001), grup kontrol (p=0.001), grup asam hyaluronat (p=0.015). Kesimpulan. Suntikan hormon pertumbuhan ke dalam sendi memiliki efektifitasyang lebih baik dibandingkan dengan Asam hyaluronat pada model osteoarthritis.Hormon pertumbuhan memberikan harapan baru sebagai alternatif dalam terapiosteoarthritis.ABSTRACT
Introduction. Osteoarthritis is a degenerative joint disorder that cause disabilityfor patients all over the globe, with an increasing number of patients. Growthhormone (GH) works trough direct and indirect effect on cartilage regeneration bychondroblast stimulation, stimulation of growth factors and morphoangiogenesisprocess. Further research is needed to know the effects of intra articular jointinjection of growth hormone using validated animal model and reliable outcomemeasurement. Methods. This study was conducted in Animal Hospital of Agricultural InstituteBogor west Java, from May to September 2015. The design of the study wasrandomized posttest only control group. Male white New Zealand rabbit (n=21)weighted 1.9-2.6kg, age 6-7months were used in this study. The sample wasrandomized and divided into three groups. All groups recieved intra articularinjection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline(0.6ml) were done at 2 weeks after collagenase injection once a week forconsecutive 3 weeks. Evaluation of weight and lameness periode is doneperiodically, histopathological and macroscopic score were done at 8 weeks sincethe first injection. Result. The lameness priode for control group is significantly longer than both ofthe experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than theHA group (P=0.04) and placebo (P=0.01). Histopathological score was also foundlowest at the GH group (p=0.001), with significant difference in control(p=0.001), and HA group (p=0.015).).Conclusion. Intraarticular injection of growth hormone is found to be moreeffective compared to hyaluronic acid on rabbit osteoarthritis model. This resultsshowed promising result for intra articular injection of GH as an alternativetreatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disabilityfor patients all over the globe, with an increasing number of patients. Growthhormone (GH) works trough direct and indirect effect on cartilage regeneration bychondroblast stimulation, stimulation of growth factors and morphoangiogenesisprocess. Further research is needed to know the effects of intra articular jointinjection of growth hormone using validated animal model and reliable outcomemeasurement. Methods. This study was conducted in Animal Hospital of Agricultural InstituteBogor west Java, from May to September 2015. The design of the study wasrandomized posttest only control group. Male white New Zealand rabbit (n=21)weighted 1.9-2.6kg, age 6-7months were used in this study. The sample wasrandomized and divided into three groups. All groups recieved intra articularinjection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline(0.6ml) were done at 2 weeks after collagenase injection once a week forconsecutive 3 weeks. Evaluation of weight and lameness periode is doneperiodically, histopathological and macroscopic score were done at 8 weeks sincethe first injection. Result. The lameness priode for control group is significantly longer than both ofthe experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than theHA group (P=0.04) and placebo (P=0.01). Histopathological score was also foundlowest at the GH group (p=0.001), with significant difference in control(p=0.001), and HA group (p=0.015).).Conclusion. Intraarticular injection of growth hormone is found to be moreeffective compared to hyaluronic acid on rabbit osteoarthritis model. This resultsshowed promising result for intra articular injection of GH as an alternativetreatment for osteoarthritis.;Introduction. Osteoarthritis is a degenerative joint disorder that cause disabilityfor patients all over the globe, with an increasing number of patients. Growthhormone (GH) works trough direct and indirect effect on cartilage regeneration bychondroblast stimulation, stimulation of growth factors and morphoangiogenesisprocess. Further research is needed to know the effects of intra articular jointinjection of growth hormone using validated animal model and reliable outcomemeasurement. Methods. This study was conducted in Animal Hospital of Agricultural InstituteBogor west Java, from May to September 2015. The design of the study wasrandomized posttest only control group. Male white New Zealand rabbit (n=21)weighted 1.9-2.6kg, age 6-7months were used in this study. The sample wasrandomized and divided into three groups. All groups recieved intra articularinjection of type 2 collagenase (Sigma® Missouri) 2mg at the left knee on day 1and 4. Injections of growth hormone (4iu), hyaluronic acid (HA) (6mg) and saline(0.6ml) were done at 2 weeks after collagenase injection once a week forconsecutive 3 weeks. Evaluation of weight and lameness periode is doneperiodically, histopathological and macroscopic score were done at 8 weeks sincethe first injection. Result. The lameness priode for control group is significantly longer than both ofthe experimental groups (p<0.001), HA (p<0.03), and GH (p<0.001).Macroscopic score evaluation taken from the lateral condyle of the left femur showed that the GH group received significantly less cartilage damage than theHA group (P=0.04) and placebo (P=0.01). Histopathological score was also foundlowest at the GH group (p=0.001), with significant difference in control(p=0.001), and HA group (p=0.015).).Conclusion. Intraarticular injection of growth hormone is found to be moreeffective compared to hyaluronic acid on rabbit osteoarthritis model. This resultsshowed promising result for intra articular injection of GH as an alternativetreatment for osteoarthritis. |
SP-Erick Wonggokusuma.pdf :: Unduh