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Artikel Jurnal :: Kembali

Mutation of the first ATG ORF-3 of Chiken Anemia Virus into ACG completely abolish the apoptin production

Afiono Agung Prasetyo, Kamahora Toshio (Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS), [Date of publication not identified] )

 Abstrak

To test effect one point mutation one the first initiation codon of Chiken Anemia Virus (CAV) open reading frame-3 (ORF-3), an opoptin knocked out expressor plasmid pCLS-VP3(-) and a CAV opoptin knocket out plasmid pCAV/Ap(-) were constructed. In both plasmids, the first ATG in COS-1 cells tranfectedwith pCLS-VP3(-) using western blotting and immunofluorescence type. After released from pCAV/Ap(-), the complete genome of CAV/Ap(-) was ligated to form the replicative form. the oppotin prodective was completely abolished in MDCC-MSB1 cells transfected with replicative form of CAV/Ap(-). The opoptin production was fully regained after a reverse mutation into CAV/Ap(-)RM. These data shows the first evidence that mutation of the first ATG of ORF-3 into ACG could completely abolish the production of apoptin

 Metadata

No. Panggil : JBB 2 (2011) (1)
Entri utama-Nama orang :
Entri tambahan-Nama orang :
Subjek :
Penerbitan : [Place of publication not identified]: Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS), [Date of publication not identified]
Sumber Pengatalogan :
ISSN : 20870183
Majalah/Jurnal : Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS)
Volume : 1 (1) April 2010. Hal. : 3-7
Tipe Konten : journal
Tipe Media : unmediated
Tipe Carrier : volume (rdacarier)
Akses Elektronik :
Institusi Pemilik : Universitas Indonesia
Lokasi : Perpustakaan UI, Lantai 4
  • Ketersediaan
  • Ulasan
No. Panggil No. Barkod Ketersediaan
JBB 2 (2011) (1) 03-19-997133513 TERSEDIA
Ulasan:
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