Mutation of the first ATG ORF-3 of Chiken Anemia Virus into ACG completely abolish the apoptin production
Afiono Agung Prasetyo, Kamahora Toshio (Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS), [Date of publication not identified]
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| To test effect one point mutation one the first initiation codon of Chiken Anemia Virus (CAV) open reading frame-3 (ORF-3), an opoptin knocked out expressor plasmid pCLS-VP3(-) and a CAV opoptin knocket out plasmid pCAV/Ap(-) were constructed. In both plasmids, the first ATG in COS-1 cells tranfectedwith pCLS-VP3(-) using western blotting and immunofluorescence type. After released from pCAV/Ap(-), the complete genome of CAV/Ap(-) was ligated to form the replicative form. the oppotin prodective was completely abolished in MDCC-MSB1 cells transfected with replicative form of CAV/Ap(-). The opoptin production was fully regained after a reverse mutation into CAV/Ap(-)RM. These data shows the first evidence that mutation of the first ATG of ORF-3 into ACG could completely abolish the production of apoptin |
| No. Panggil : | JBB 2 (2011) (1) |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Subjek : | |
| Penerbitan : | [Place of publication not identified]: Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS), [Date of publication not identified] |
| Sumber Pengatalogan : | |
| ISSN : | 20870183 |
| Majalah/Jurnal : | Indonesian Center for Biotechnology and Biodiversity Research and Development (UNS) |
| Volume : | 1 (1) April 2010. Hal. : 3-7 |
| Tipe Konten : | journal |
| Tipe Media : | unmediated |
| Tipe Carrier : | volume (rdacarier) |
| Akses Elektronik : | |
| Institusi Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI, Lantai 4 |
| No. Panggil | No. Barkod | Ketersediaan |
|---|---|---|
| JBB 2 (2011) (1) | 03-19-997133513 | TERSEDIA |
| Ulasan: |
| Tidak ada ulasan pada koleksi ini: 20424726 |
