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In Vitro Propagation of Artemisia annua. Rossa Yunita and Endang G. Lestari. Artemisinin, an anti-malarial medi- cine isolated from the annual wormwood Artemisia annua, has a marked activity against chloroquine-resistant and chloroquine-sensitive strains of Plasmodium falciparum. This compound is useful for treatment of cerebral malaria. An in vitro propagation system for A. annua has been de- veloped. Shoots were induced by culturing seeds of A. annua on a MS medium containing BAP (0, 0.1, 0.3, 0.5 mg/l). Shoots were also formed on each seedling cultured on the same medium. Root formations were obtained from shoots that were subcultured on a MS medium containing IBA (0, 1.0, 1.5, 2 mg/l). The results showed that MS medium supplemented with BAP 0.3 mg/l was the best medium for induction and multiplication of the shoots, while the MS medium supplemented with IBA (1 mg/l) was good for root formations. |
