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Study on the structure and the mechanism of the formation of early caries is an important premise for evaluatingthe caries-preventive methods. Objective: To describe micrograph images of experimental carious lesionsat early stage in permanent teeth in Vietnamese. Methods: There were 60 permanent extracted premolars frompatients aged 18-25 used in this study. After cleaning and removing the remaining soft tissue, each tooth waspainted with acid-resistant coating leaving an enamel window of approximately 1 × 1mm2 on the buccal surface.The artificial carious lesion was performed by soaking the teeth for 96 hours in the demineralization solution andevaluated using the ICDAS criteria. The tooth specimen was observed under a scanning electronic microscope(SEM). Results: The experiment resulted 66.7% of ICDAS 1 and 33.3% ICDAS 2 carious lesions. The meandepth and area of the lesions were 107.6µm and 1.14 µm2, respectively. The difference in depth and area of lesionsbetween group ICDAS 1 and ICDAS 2 was significantly statistical with p=0.0001. The micrographs showed thatafter being soaked in demineralization solution, enamel surface became rough, enamel rods lost the minerals inthe body more than in the sheath, cross-sectional images showed widened gaps between the rods due to demineralization.There was a difference in demineralization degree between group ICDAS 1 and 2. Conclusion: Theimage of carious lesions was gained after the teeth were soaked in demineralization solution. It showed differentcharacteristics of the resulted lesions based on ICDAS criteria for early carious lesions using scanning electronmicroscope. |
