ABSTRAK
Bakteri pendegradasi senyawa hidrokarbon dapat diisolasi dari daerah yangterkontaminasi polutan, seperti habitat mangrove. Isolat bakteri SM1_7 telahdiisolasi dari habitat mangrove Suaka Margasatwa Muara Angke, Jakarta Utara.Penelitian bertujuan untuk menganalisis kemampuan degradasi senyawahidrokarbon dan mengarakterisasi isolat bakteri SM1_7. Pengukuran pertumbuhandilakukan dengan metode viable plate count dan analisis senyawa hidrokarbondengan GC/MS. Karakterisasi bakteri dilakukan dengan pengecatan Gram,pengamatan morfologi, dan karakterisasi biokimia. Hasil pengukuranpertumbuhan menunjukkan bahwa isolat bakteri SM1_7 mampu tumbuh dimedium Bushnell Haas + 1% (v/v) minyak diesel yang menunjukkan peningkatan2,3x109 CFU/mL menjadi 3,31x1011 CFU/mL setelah inkubasi 12 jam. Isolatbakteri SM1_7 mampu mendegradasi senyawa hexadecane (C16H35) sebanyak13,95%, heptadecane (C17H36) sebanyak 17,66%, dan eicosane (C20H42) sebanyak19,14%. Hasil karakterisasi fenotipik bakteri menunjukkan bahwa isolat bakteriSM1_7 diduga termasuk ke dalam genus Pseudomonas.
ABSTRACT
Hydrocarbon degrading bacteria can be isolated from contaminated areas, such asmangrove. Bacteria isolate SM1_7 has been isolated from mangrove habitat SuakaMargasatwa Muara Angke, North Jakarta. The objectives of the research is toanalyze the capability of isolate SM1_7 for degrading hydrocarbons andcharacterize bacterial isolate SM1_7. Growth measurements was performed usingviable plate count method and analysis of hydrocarbon degradation was carriedout using GC/MS. Bacterial characterization was done using Gram stains,observing morphological characteristics, and analysis of biochemicalcharacteristics. The results show that bacteria isolate SM1_7 is able to grow inBushnell Haas medium + 1% (v/v) diesel oil displaying an increase from 2,3x109CFU/mL to 3,31x1011 CFU/mL after 12 hours incubation. Bacteria isolate SM1_7is able to degrade hexadecane (C16H35) 13,95%, heptadecane (C17H36) 17,66%,and eicosane (C20H42) 19,14%. The result of phenotypic characterization showedthat bacteria isolate SM1_7 is assumed to be from genus Pseudomonas. |
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