Efek ekstrak etil asetat daun lamun [enhalus acoroides l.f. royle] terhadap analisis semen, morfologi sel leydig, dan stres oksidatif pada mencit jantan tua = Effect of ethyl acetate extract of lamun leaves enhalus acoroides l f royle on semen analysis leydig cell morphology and oxidative stress in old male mice / Novita Rina Antarsih
Novita Rina Antarsih;
Lutfi Hardiyanto, supervisor; Sri Widia Jusman, supervisor; Arleni, examiner; Ani Retno Prijanti, examiner; Wawaimuli Arozal, examiner
([Publisher not identified]
, 2016)
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ABSTRAK LATAR BELAKANG: Laki-laki dengan penuaan pada umumnya mengalamipenurunan kualitas hidup dan infertilitas, penyebabnya berkaitan dengan degenerasisel Leydig akibat akumulasi stres oksidatif sehingga terjadi penurunan kadartestosteron. Daun Enhalus acoroides dilaporkan mengandung antioksidan yang dapatmenangkal radikal bebas dan fitosterol yang dapat diubah menjadi testosteron.Penelitian ini dilakukan untuk mengetahui efek ekstrak etil asetat daun lamun[Enhalus acoroides (L.f.) Royle] terhadap analisis semen, morfologi sel Leydig, danstres oksidatif mencit jantan tuaBAHAN DAN CARA KERJA: Sampel berjumlah 32 ekor mencit jantan galur DDYyang diberikan ekstrak daun Enhalus acoroides selama 14 hari. Mencit dibagi dalam8 kelompok perlakuan masing masing terdiri dari 4 kali ulangan yang terdiri dariDK1 = dewasa kontrol 1 (mencit dewasa tanpa perlakuan); DK2=dewasa kontrol 2(mencit dewasa minyak zaitun); DP1=dewasa perlakuan 1 (mencit dewasa ekstrak 25mg/KgBB); DP2=dewasa perlakuan 2 (mencit dewasa ekstrak 50 mg/KgBB);TK1=tua kontrol 1 (mencit tua tanpa perlakuan); TK2=tua kontrol 2 (mencit tuaminyak zaitun); TP1=tua perlakuan 1 (mencit tua ekstrak 25 mg/KgBB); TP2=tuaperlakuan 2 (mencit tua ekstrak 50 mg/KgBB).HASIL: Peningkatan motilitas dan viabilitas spermatozoa mencit tua pada dosis 50mg/KgBB, peningkatan morfologi spermatozoa normal pada mencit tua dengan dosis25mg/KgBB dan penurunan konsentrasi spermatozoa mencit tua pada dosis 25mg/KgBB. Adanya peningkatan sel Leydig tua pada dosis 25 mg/KgBBKESIMPULAN: Pemberian ekstrak daun Enhalus acoroides meningkatkan kualitasspermatozoa. Namun tidak ada perbedaan dalam penundaan degenerasi sel Leydigdewasa serta tidak ada perbedaan pada tingkat stres oksidatif ABSTRACT Background: The men aging process occurs generally due to decreased quality oflife and infertility, the cause associated with Leydig cell degeneration due toaccumulation of oxidative stress resulting in a decrease in testosterone levels. At thistime the administration of synthetic antioxidants is limited because of the risk ofcancer, on the other hand administration of synthetic testosterone cause the cessationof endogenous testosterone production and long-term risk. Lamun leaves Enhalusacoroides reported to contain antioxidants which can counteract free radicals andphytosterols that can be converted into testosterone. Therefore, this study wasconducted to determine effect of ethyl acetate extract of lamun leaves Enhalusacoroides on semen analysis, Leydig cell morphology, and oxidative stress in oldmale miceMethods: The sample amounted to 32 mice (mus Musculus) strain DDY male givenextract of the leaves of seagrass for 14 days. Mice were divided into eight treatmentgroups each consisting of four replications. Four groups are composed of adult micewhich consist of DK1=adult control 1, DK2= adult control 2 (adult mice with oliveoil), DP1= adult treatment 1 (extract 25 mg/KgBW), DP2=adult treatment 2 (extract50 mg/KgBW). 4 groups male older mice consist of TK1 = old control 1, TK2= oldcontrol 2 (old mice with olive oil), TP1= old treatment 1 (extract 25 mg/KgBW),TP2= old treatment 2 (extract 50 mg/KgBW).Results: An increased motility and viability of sperm at doses of 50 mg/KgBW,increased morphology of sperm normally at doses of 25 mg/KgBW, and decreasedthe concentration of sperm at doses of 25 mg/KgBW. An increased in aged Leydigcells at doses 25 mg/KgBWConclusion: The extract of lamun leaves can qualitatively improve sperm, howevernot significant in delays degeneration of adult Leydig cells and not significantdecreased levels of oxidative stress in old male mice |
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| No. Panggil : | T-Pdf |
| Entri utama-Nama orang : | |
| Entri tambahan-Nama orang : | |
| Entri tambahan-Nama badan : | |
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| Penerbitan : | [Place of publication not identified]: [Publisher not identified], 2016 |
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| Bahasa : | ind |
| Sumber Pengatalogan : | |
| Tipe Konten : | text |
| Tipe Media : | computer |
| Tipe Carrier : | online resource |
| Deskripsi Fisik : | xvii, 139 pages : illustration ; 28 cm + appendix |
| Naskah Ringkas : | |
| Lembaga Pemilik : | Universitas Indonesia |
| Lokasi : | Perpustakaan UI, Lantai 3 |
| No. Panggil | No. Barkod | Ketersediaan |
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| T-Pdf | 15-17-818072609 | TERSEDIA |
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| Tidak ada ulasan pada koleksi ini: 20432421 |
