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Background: The purpose of this study was to provide a reference of chronic diabetes complications by investigatingthe prolonged hyperglycemia effects on hematological, biochemical and histopathological changes (liver, kidney,spleen, cardiac muscle, adrenal gland, and endocrine pancreas) in diabetic rats induced by streptozotocin. Methods:Ten adult female Sprague-Dawley of uniform age were divided into two Groups. Group 1 was made diabetic by singleintraperitoneal injection of streptozotocin (60 mg/kg/bw) whereas Group 2 served as control. After six months, the ratswere anesthetized using pentobarbital. Cardiac puncture was performed to get 3 ml of the blood sample; following 12hours of an overnight fast. Serum chemistry test and complete blood analysis for lipid profile and blood glucose test;liver and renal functions were performed. Tissue specimens of liver, kidney, spleen, cardiac muscle, adrenal gland, andendocrine pancreas were fixed in 10% formal saline and processed for histological study. Results: There were severehistopathological changes in the affected organs; and the presence of a significant abnormality of lipid profile, liver, andrenal functions. Conclusions: The presence of histopathological changes with abnormal biochemical changes is relatedto the chronic absence of insulin production in the destroyed β –cells which reflect the diabetic complications in ahuman being. |
