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Objective: There are many factors that govern growth and resistant of Salmonella typhi. A study had reported that theuse of sodium benzoate caused antibiotic resistant. However, no study has directly evaluated the effect of sodiumbenzoate exposure on S. typhi sensitivity to chloramphenicol. The aim of this study was to evaluate the resistance orsensitivity of S. typhi to chloramphenicol after sodium benzoate exposure. Methods: The study was conducted in sevengroups: three treatment groups (sodium benzoate insensitive S. typhi+8 μg/mL, 16 μg/mL, and 32 μg/mL ofchloramphenicol), three positive control groups (sodium benzoate sensitive S. typhi+8 μg/mL, 16 μg/mL, and 32 μg/mLof chloramphenicol), and one negative control groups (sodium benzoate sensitive S. typhi+0 μg/mL of chloramphenicol).The effect of sodium benzoate exposure to S. typhi sensitivity to chloramphenicol was measured after 24 hours.Spearman test was used to analyzed this association. Results: In this study, we found that the average S. typhi growth inthe treatment groups (A, B, C) was 445 CFU/mL, 385 CFU/mL, and 171 CFU/mL, respectively. While in the positivecontrol group (D, E, F) was not obtained any S. typhi growth. Average S. typhi growth in the negative control group was430 CFU/mL. We found that sodium benzoate exposure inhibited S. typhi growth and affected S. typhi sensitivity tochloramphenicol (p < 0.05). In addition, we found that 32 μg/mL chloramphenicol had the highest mean differencevalue, so this showed that the dose 32 μg/mL of chloramphenicol had the best effectiveness of various treatment groups(p < 0.05). Conclusions: Sodium benzoate exposure can inhibit S. typhi growth and cause S. typhi resistant tochloramphenicol. |
